Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

Abstract

This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.

Electrical therapies act on the Ca2+/CaM signaling pathway to enhance bone regeneration with bioactive glass [S53P4] and allogeneic grafts

“…Zhang et al51 demonstrated in ADSC (adipose-derived mesenchymal stem cells) that ES activates the flow of calcium through voltage-dependent channels, stimulating osteogenesis.Both TNAP and BSP-II are involved in the mineralization, organization, and calcification of the bone matrix. A time-dependent increase in the activity of these proteins was observed in the repair of bone fractures [52][53][54]. The analyses for TNAP and BSP-II expression in our study highlighted that grafting with BB was efficient in with ES and EM in the last experimental period.…”

Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

“…Mineral trioxide aggregate (MTA) was developed due to its tight sealing potential and favorable biological properties; it also has odontoblastic and cementoblastic properties [ 5 , 6 , 7 ]. Previous studies have demonstrated favorable clinical outcomes following direct pulp capping with MTA compared to calcium hydroxide [ 8 , 9 , 10 , 11 ].…”

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

“…A positive effect of MTA on viability of rat BMSCs is observed up to day 7, which is sooner than in human BMSCs. Simultaneously, MTA stimulates rat BMSC differentiation into osteoblast-like cells over 7 days, which is confirmed by enhanced ALP staining and upregulation of gene expression levels of bone morphogenetic protein 2 ( BMP-2), ALP , bone sialoprotein ( BSP ) and osteocalcin (OC ) ( 109 ). Low concentrations of MTA-conditioned media (2.000, 0.200, 0.020 and 0.002 mg/ml) are not toxic to BMSCs and 0.02 mg/ml MTA is the optimal concentration to upregulate odonto/osteogenic capacity of BMSCs originating from rat mandible; this finding was supported by enhanced Alp activity, calcified nodule formation and expression levels of odonto/osteoblastic genes in BMSCs, such as ALP, RUNX2, OSX, OCN and DSPP .…”