Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

Kim, Yemi; Lee, Dong-Hee; Song, Dani; Kim, Hye Min; Kim, Sin Young

doi:10.3390/ma13183925

Cited by 27 publications

(28 citation statements)

References 35 publications

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“…This is due to the formation of CH following the hydration reaction, which created the premise of a high pH with antibacterial activity and the formation of calcium ions that induced morpho-differentiation and proliferation of odontoblast-like cells at the level of pulp exposure site [ 148 ]. Even though TheraCal LC contains resin in its composition, and there is a risk of remaining unpolymerized resin monomers, it has good biocompatibility compared to CH [ 149 ]. However, one study indicated that after 72 h, TheraCal LC showed a decrease in the percentage of cell viability, similar to CH [ 150 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

et al. 2021

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Dental pulp vitality is a desideratum for preserving the health and functionality of the tooth. In certain clinical situations that lead to pulp exposure, bioactive agents are used in direct pulp-capping procedures to stimulate the dentin-pulp complex and activate reparative dentinogenesis. Hydraulic calcium-silicate cements, derived from Portland cement, can induce the formation of a new dentin bridge at the interface between the biomaterial and the dental pulp. Odontoblasts are molecularly activated, and, if necessary, undifferentiated stem cells in the dental pulp can differentiate into odontoblasts. An extensive review of literature was conducted on MedLine/PubMed database to evaluate the histological outcomes of direct pulp capping with hydraulic calcium-silicate cements performed on animal models. Overall, irrespective of their physico-chemical properties and the molecular mechanisms involved in pulp healing, the effects of cements on tertiary dentin formation and pulp vitality preservation were positive. Histological examinations showed different degrees of dental pulp inflammatory response and complete/incomplete dentin bridge formation during the pulp healing process at different follow-up periods. Calcium silicate materials have the ability to induce reparative dentinogenesis when applied over exposed pulps, with different behaviors, as related to the animal model used, pulpal inflammatory responses, and quality of dentin bridges.

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Section: Discussionmentioning

confidence: 99%

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

et al. 2021

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“…Lipski et al, reported that Biodentine had an 82% success rate at 1–1.5 years after direct pulp capping, and the success rate was 90.0% among patients under 40 years of age [ 26 ]. Biodentine was associated with higher cell proliferation and migration rates than the rates observed with other pulp-capping materials [ 18 , 27 , 28 ]. The effects of Biodentine on dentin bridge formation were similar to those observed with ProRoot MTA [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…The hDPSC line was established at Top Cell Bio, Inc (Seoul, Korea). The experiments were performed with hDPSCs that had been passaged 4 times, as described previously [ 18 ]. Cells were grown in HyClone Minimum Essential Medium (α-MEM; GE Healthcare Life Sciences, Pittsburgh, PA, USA) supplemented with HyClone 10% fetal bovine serum (GE Healthcare Life Sciences), 100 U/mL penicillin, and 100 μg/mL streptomycin.…”

Section: Methodsmentioning

confidence: 99%

“…These eluates were used in subsequent experiments to test whether the different materials affected odontogenic differentiation. On days 3 and 6, we evaluated intracellular alkaline phosphatase (ALP) activity in hDPSCs with the Senso-Lyte ® p-nitrophenylphosphate (pNPP) alkaline phosphatase assay kit (AnaSpec, Fremont, CA, USA), as described previously [ 18 ]. Briefly, cells were fixed in 4% paraformaldehyde (Biosesang) for 1–2 min, then rinsed with TBST buffer (1X Tris-Buffered Saline, 0.05% Tween 20 Detergent).…”

Section: Methodsmentioning

confidence: 99%

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Biological Characteristics and Odontogenic Differentiation Effects of Calcium Silicate-Based Pulp Capping Materials

et al. 2021

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We compared calcium silicate-based pulp capping materials to conventional calcium hydroxide in terms of their biological properties and potential effects on odontogenic differentiation in human dental pulp stem cells (hDPSCs). We cultured hDPSCs on disks (7-mm diameter, 4-mm high) of ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), or Dycal (Dentsply Tulsa Dental Specialties). Cell viability was assessed with cell counting (CCK) and scanning electron microscopy (SEM). Odontogenic activity was assessed by measuring alkaline phosphatase (ALP) activity and gene expression (quantitative real-time PCR). CCK assays showed that Dycal reduced cell viability compared to the other materials (p < 0.05). SEM showed low and absent cell attachment on TheraCal LC and Dycal disks, respectively. hDPSCs exposed to TheraCal LC and Dycal showed higher ALP activity on day 6 than the control group (p < 0.05). The day-9 Runx2 expression was higher in the ProRoot MTA and TheraCal LC groups than in the control group (p < 0.05). On day 14, the ProRoot MTA group showed the highest dentin sialophosphoprotein levels (not significant; p > 0.05). In conclusion, various pulp capping materials, except Dycal, exhibited biological properties favorable to hDPSC viability. ProRoot MTA and TheraCal LC promoted higher Runx2 expression than Biodentine. Future studies should explore the odontogenic potential of pulp capping materials.

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“…Similar results were obtained when hDPSCs were cultured in medium conditioned with Biodentine or ProRoot MTA 47 . When transwell membrane was used to avoid direct contact between the biomaterials and hDPSCs, there was no significant difference in cell viability and cell migration between ProRoot MTA and Biodentine 48 .…”

Section: Discussionmentioning

confidence: 99%

The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

Rathinam

Govindarajan

Rajasekharan

et al. 2021

Sci Rep

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Calcium (Ca2+) signalling plays an indispensable role in dental pulp and dentin regeneration, but the Ca2+ responses of human dental pulp stem cells (hDPSCs) stimulated with tricalcium silicate-based (TCS-based) dental biomaterials remains largely unexplored. The objective of the present study was to identify and correlate extracellular Ca2+ concentration, intracellular Ca2+ dynamics, pH, cytotoxicity, gene expression and mineralization ability of human dental pulp stem cells (hDPSCs) stimulated with two different TCS-based biomaterials: Biodentine and ProRoot white MTA. The hDPSCs were exposed to the biomaterials, brought in contact with the overlaying medium, with subsequent measurements of extracellular Ca2+ and pH, and intracellular Ca2+ changes. Messenger RNA expression (BGLAP, TGF-β, MMP1 and BMP2), cytotoxicity (MTT and TUNEL) and mineralization potential (Alizarin red and Von Kossa staining) were then evaluated. Biodentine released significantly more Ca2+ in the α-MEM medium than ProRoot WMTA but this had no cytotoxic impact on hDPSCs. The larger Biodentine-linked Ca2+ release resulted in altered intracellular Ca2+ dynamics, which attained a higher maximum amplitude, faster rise time and increased area under the curve of the Ca2+ changes compared to ProRoot WMTA. Experiments with intracellular Ca2+ chelation, demonstrated that the biomaterial-triggered Ca2+ dynamics affected stem cell-related gene expression, cellular differentiation and mineralization potential. In conclusion, biomaterial-specific Ca2+ dynamics in hDPSCs determine differentiation and mineralization outcomes, with increased Ca2+ dynamics enhancing mineralization.

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Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

Cited by 27 publications

References 35 publications

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

Biological Characteristics and Odontogenic Differentiation Effects of Calcium Silicate-Based Pulp Capping Materials

The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

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