Induction of micronuclei in tadpoles of<b><i>Odontophrynus americanus</i></b>(Amphibia: Leptodactylidae) by the pyrethroid insecticide cypermethrin

Cabagna, Mariana; Peltzer, Paola M.; Attademo, Andrés M.; Ale, Ezequiel

doi:10.1080/02772240600903805

Cited by 38 publications

(11 citation statements)

References 38 publications

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“…If we consider the reported LC50 values for fish and tadpoles, H. pulchellus is a species with low sensitivity to this insecticide. The reported sublethal effects on amphibians include cellular apoptosis (Cabagna et al 2006), micronuclei formation (Campana et al 2003), inhibition of cholinesterase activity (Khan et al 2003), decrease in hatching success, delays in metamorphosis, reduction in body growth, and abnormalities (Greulich and Pflugmacher 2003) within a concentration range of 0.1 and 10 lg CY/L. These results are in agreement with behavioral effects seen in this study at concentrations of 3 lg CY/L, the inhibition of body size at 3.44 lg CY/L, and the presence of abnormalities at 0.34 lg CY/L.…”

Section: Discussionmentioning

confidence: 99%

“…Salibián (1992) reports neurotoxicity caused by deltamethrin in Rhinella arenarum, and Campana et al (2003) the induction of micronuclei caused by k-cyhalothrin exposure in Lithobates catesbeianus. Also, induction of micronuclei was observed in Odontophrynus americanus exposed to CY (Cabagna et al 2006). Particularly, data on the lethal effects of CY on anuran species are scarce.…”

Section: Introductionmentioning

confidence: 95%

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Lethal and sublethal effects of cypermethrin to Hypsiboas pulchellus tadpoles

2010

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The study of the effects of the insecticide cypermethrin (CY) technical grade and its Sherpa® commercial formulation on Hypsiboas pulchellus tadpoles assessing lethality, behavior, growth, and abnormalities under standardized laboratory conditions is reported. Observed behaviors were identified and categorized by means of a ranking system according to the loss of mobility. Results of acute lethal effects indicate higher potency for Sherpa® at this level of assessment. All effects on behavior showed an increasing degree of injury as insecticide concentration increased. Organisms exposed to technical grade CY showed lower body length with respect to controls from 3.44 µg CY/L to higher concentrations, whereas those exposed to Sherpa® exhibited lower growth from 0.83 µg CY/L. Both forms of the tested insecticide caused abnormalities between 0.34 and 4.18 µg CY/L, but 100% of malformed individuals was detected from 34.4 µg CY/L for those exposed to the technical grade CY, and from 8.36 µg CY/L for those exposed to Sherpa®. This study proposes the use of easily identifiable and distinguishable sublethal end-points. The high input loads of CY in natural environments, the detected concentrations in the field, in addition to the low levels of this insecticide required to induce sublethal effects (which could eventually lead to death), allow for the conclusion that the insecticide is a risk factor for amphibians inhabiting agroecosystems.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Lethal and sublethal effects of cypermethrin to Hypsiboas pulchellus tadpoles

2010

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“…Anuran erythrocytes have a high incidence of micronucleus and nuclear abnormalities after the exposure to various contaminants (Campana et al, 2003;Lajmanovich et al, 2005b;Cabagna et al, 2006). For this, are commonly useful for knowing the relation between the organisms and their medium, and with that, the health of the environment (Hoffman et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Health status of tadpoles and metamorphs of Rhinella arenarum (Anura, Bufonidae) that inhabit agroecosystems and its implications for land use

Babini

Bionda

Salas

et al. 2015

Ecotoxicology and Environmental Safety

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“…Slides were coded, randomized, and blind-scored by a single researcher. The criteria employed for MNi identification were as follows: a diameter smaller than 1/3 of the main nuclei diameter, nonrefractability, staining intensity similar to or lighter than that of the main nuclei, no connection or link with the main nuclei, and no overlapping with the main nuclei (Fenech 2000;Ferreira et al 2004;Cabagna et al 2006).…”

Section: Micronucleus Analysismentioning

confidence: 99%

“…The slides were stained 12 h after alcohol fixation, with Giemsa 10 %, for 10 min. The frequency of micronuclei (MNi) was determined by analyzing 1,000 erythrocytes from each tadpole, using ×1000 magnification as suggested elsewhere (Cabagna et al 2006) and expressed as the total number of MNi/1000 cells. Slides were coded, randomized, and blind-scored by a single researcher.…”

Section: Micronucleus Analysismentioning

confidence: 99%

Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

Gonçalves

Vieira

Maciel

et al. 2014

Environ Sci Pollut Res

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The aim of the study was to use the comet assay (single-cell gel electrophoresis) and micronucleus test to assess the extent of genomic damage in the whole blood of Dendropsophus minutus from agroecosystems with great use of agrochemicals and to compare the results to those obtained from animals living in unpolluted areas. Our results indicated that specimens of D. minutus collected in perturbed areas exhibited higher amounts of DNA damage in blood cells in comparison to animals from areas free of agricultural activities. The average and standard deviation of all comet assay parameters (tail length, percentage of DNA in the tail, and olive tail moment) and micronuclei frequency were significantly higher in specimens collected in perturbed areas than in the animals from preserved areas. Our study showed that animals from perturbed areas, such as agroecosystems, tend to have higher amounts of DNA damage than animals from reference areas. Moreover, we can conclude that D. minutus tadpoles could be included as a model organism in biomonitoring studies.

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Induction of micronuclei in tadpoles ofOdontophrynus americanus(Amphibia: Leptodactylidae) by the pyrethroid insecticide cypermethrin

Cited by 38 publications

References 38 publications

Lethal and sublethal effects of cypermethrin to Hypsiboas pulchellus tadpoles

Lethal and sublethal effects of cypermethrin to Hypsiboas pulchellus tadpoles

Health status of tadpoles and metamorphs of Rhinella arenarum (Anura, Bufonidae) that inhabit agroecosystems and its implications for land use

Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

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