Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

Gonçalves, Macks Wendhell; Vieira, Thiago Bernardi; Maciel, Natan Medeiros; Carvalho, Wanessa Fernandes; Lima, Lorena S.; Gambale, Priscilla Guedes; Cruz, Aparecido Divino da; Nomura, Fausto; Silva, Daniela de Melo e

doi:10.1007/s11356-014-3682-1

Cited by 21 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pesticides can negatively affect amphibian individuals and populations, specifically their survival. They alter the larval growth and development rates; change morphology, physiology, and behavior; and induce genetic damage to the DNA, thus reducing the reproductive output (Carey and Bryant 1995;Jha 2008;Sparling et al 2010;Gonçalves et al 2014). Moreover, environmental pollutants or stressors are known to affect the immune system of amphibians (Christin et al 2004;Jantawongsri et al 2013).…”

Section: Responsible Editor: Thomas Hutchinsonmentioning

confidence: 99%

Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

Iglesias

Franco‐Belussi

Moreno

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

Glyphosate (GLY) is the most used herbicide worldwide and its effects on anurans are well known. Pollutants can cause physiological and morphological effects. Therefore, this study evaluated the effects of GLY on hepatic melanomacrophages as a response to environmental stressors. Three treatments were exposed to different concentrations of pure GLY (100, 1000, and 10,000 μg g(-1), respectively), and there was also a control group. After the experimental time, liver and blood were analyzed. Melanomacrophages (MMCs) were located between the hepatocyte cordons, close to sinusoids. GLY increased the melanin area in MMCs of Leptodactylus latinasus exposed since lowest concentration until highest concentration. GLY also changed the occurrence of hepatic catabolism pigments into melanomacrophages and erythrocyte nuclear abnormalities; therefore, it can interfere with the hepatic metabolism. In conclusion, GLY promotes alterations in the hepatic tissue and erythrocyte nuclear abnormalities. Furthermore, MMCs may be useful as morphological responses of GLY effects.

show abstract

Section: Responsible Editor: Thomas Hutchinsonmentioning

confidence: 99%

Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

Iglesias

Franco‐Belussi

Moreno

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…The conducted studies mainly focused on the evaluation of environmental stressors such as agrochemicals and heavy metals in vivo. [223,[227][228][229][230][231][232][233][234][235][236][237], fungicides (captan) [238], pesticides [239,240], antiparasitic agents [239], persistent organic pollutants (POPs) [219] as well as other agrochemicals [241] and poultry litter [242]. These studies indicated the DNA damaging effects of selected chemicals as well as using amphibians as sentinel organisms for the evaluation of environmental pollution.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

View full text Add to dashboard Cite

The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

show abstract

“…One other widely used procedure is the micronucleus test (Benvindo‐Souza et al, 2020), which assesses the damage caused during cell division through the erroneous segregation or breakage of the chromosomes (Benvindo‐Souza et al, 2019; Bolognesi et al, 2011). Anuran erythrocytes typically present a high frequency of both nuclear abnormalities and micronuclei following exposure to contaminants (Amaral et al, 2018; Ascoli‑Morrete et al, 2022; Gonçalves et al, 2015), and some organs, in particular the liver, may be affected directly by these substances.…”

Section: Introductionmentioning

confidence: 99%

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

show abstract

Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

Cited by 21 publications

References 43 publications

Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Contact Info

Product

Resources

About