Induction of liver cytochrome P450s by Danshen–Gegen formula is the leading cause for its pharmacokinetic interactions with warfarin

Zhang, Zhen; Ge, Beikang; Zhou, Limin; Lam, Tai Ning; Zuo, Zhong

doi:10.1016/j.jep.2014.04.047

Cited by 21 publications

(15 citation statements)

References 55 publications

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“…Rat livers were perfused with saline before being cut into pieces and kept on ice for storage at −80 °C. Liver microsomes were prepared by sequential centrifugation as described previously . Protein concentrations of the prepared liver microsomes were measured using the Bio‐Rad protein assay kit and analysed by the micro‐plate reader .…”

Section: Methodsmentioning

confidence: 99%

“…The activities of rCyps for metabolizing warfarin were evaluated using rat liver microsome incubation described in our previous study . The activities of rCyps were reflected by the amount of mono‐hydroxywarfarin metabolites formed during microsome incubation.…”

Section: Methodsmentioning

confidence: 99%

“…The sequence of the sense and antisense primers for rat VKOR gene (VKORC1) , TM gene , rCyp1a1 , rCyp2b1 , rCyp2c6 , rCyp2c11 and GAPDH gene were designed as described previously. The fold change of target gene expression level from different treatment groups was determined using the equation as follow: Fold change =

2^{∆ ((),, ∆ ∁_{t})},

where

∆ ∁_{t} = ∁_{normalt ((),, target)} yellow- ∁_{normalt ((),, GAPDH)}

and

∆ ((),, ∆ ∁_{t}) = ∆ ∁_{normalt ((),, treated)} yellow- ∆ ∁_{normalt ((),, control)}

.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous findings identified the herb–drug interaction between Pueraria lobata (Willd), Ohwi (Gegen) and warfarin in rats . It seems reasonable to hypothesize that puerarin, the most abundant component in Gegen, may contribute to such interaction.…”

Section: Introductionmentioning

confidence: 92%

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Puerarin offsets the anticoagulation effect of warfarin in rats by inducing rCyps, upregulating vitamin K epoxide reductase and inhibiting thrombomodulin

Zhang

Lam

et al. 2017

Biopharm & Drug Disp

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

2^{∆ ((),, ∆ ∁_{t})},

where

∆ ∁_{t} = ∁_{normalt ((),, target)} yellow- ∁_{normalt ((),, GAPDH)}

and

∆ ((),, ∆ ∁_{t}) = ∆ ∁_{normalt ((),, treated)} yellow- ∆ ∁_{normalt ((),, control)}

.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

See 2 more Smart Citations

Puerarin offsets the anticoagulation effect of warfarin in rats by inducing rCyps, upregulating vitamin K epoxide reductase and inhibiting thrombomodulin

Zhang

Lam

et al. 2017

Biopharm & Drug Disp

Self Cite

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“…By adding warfarin to the freshly obtained plasma, a 12–22% increase of unbound warfarin concentrations was observed. Due to the highly bound fraction of warfarin in rat plasma (92%), the authors recommended that this change of unbound warfarin levels cannot be overlooked and concomitant use of Danshen-Gegen and warfarin for treating cardiovascular disease should be avoided [29]. …”

Section: Pharmacokinetic Interaction Caused By Salvia Miltiorrhizamentioning

confidence: 99%

Salvia miltiorrhizaRoots against Cardiovascular Disease: Consideration of Herb-Drug Interactions

Chen¹,

Tian

2017

BioMed Research International

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Salvia miltiorrhiza root (Danshen) is widely used in Asia for its cardiovascular benefits and contains both hydrophilic phenolic acids and lipophilic tanshinones, which are believed to be responsible for its therapeutic efficacy. This review summarized the effects of these bioactive components from S. miltiorrhiza roots on pharmacokinetics of comedicated drugs with mechanic insights regarding alterations of protein binding, enzyme activity, and transporter activity based on the published data stemming from both in vitro and in vivo human studies. In vitro studies indicated that cytochrome P450 (CYP450), carboxylesterase enzyme, catechol-O-methyltransferase, organic anion transporter 1 (OAT1) and OAT3, and P-glycoprotein were the major targets involved in S. miltiorrhiza-drug interactions. Lipophilic tanshinones had much more potent inhibitory effects towards CYPs activities compared to hydrophilic phenolic acids, evidenced by much lower Ki values of the former. Clinical S. miltiorrhiza-drug interaction studies were mainly conducted using CYP1A2 and CYP3A4 probe substrates. In addition, the effects of coexisting components on the pharmacokinetic behaviors of those noted bioactive compounds were also included herein.

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From Traditional Herbal Medicine to Rational Drug Discovery: Strategies, Challenges, and Future Perspectives

Singh

Pathak

Rai

2022

Rev. Bras. Farmacogn.

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Induction of liver cytochrome P450s by Danshen–Gegen formula is the leading cause for its pharmacokinetic interactions with warfarin

Cited by 21 publications

References 55 publications

Puerarin offsets the anticoagulation effect of warfarin in rats by inducing rCyps, upregulating vitamin K epoxide reductase and inhibiting thrombomodulin

Puerarin offsets the anticoagulation effect of warfarin in rats by inducing rCyps, upregulating vitamin K epoxide reductase and inhibiting thrombomodulin

Salvia miltiorrhizaRoots against Cardiovascular Disease: Consideration of Herb-Drug Interactions

From Traditional Herbal Medicine to Rational Drug Discovery: Strategies, Challenges, and Future Perspectives

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