Induction of DNA damage in Ehrlich ascites tumour cells by exposure to eupatoriopicrin

Woerdenbag, Herman J.; Vanderlinde, Jcc; Kampinga, Harm H.; Malingre, Tm; Konings, Awt

doi:10.1016/0006-2952(89)90466-8

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Severe decrease of tumour cell survival in vitro was associated with eupatoriopicrin concentrations ranging from 1–10 μg/ml [12, 13] which was correlated with induction of DNA damage [12]. Also, anti-proliferative effects on distinct cancer cell lines have been described for centaureidin concentrations below 1 μg/ml [18] as well as for jaceosidin in the concentration range of 20–50 μg/ml [19] and hispidulin for 4–30 μg/ml [21].…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity of Eupatorium cannabinum L. ethanolic extract against colon cancer cells and interactions with Bisphenol A and Doxorubicin

Ribeiro

Ressurreição

Viegas

et al. 2014

BMC Complement Altern Med

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BackgroundEupatorium cannabinum L. has long been utilized in traditional medicine, however no information is available regarding cellular effects of full extracts. Here we assessed the effects of E. cannabinum ethanolic extract (EcEE) on the colon cancer line HT29. Potential interactions with bisphenol A (BPA) a synthetic phenolic compound to which humans are generally exposed and a commonly used chemotherapeutic agent, doxorubicin (DOX) were also evaluated.MethodsHT29 cells were exposed to different concentrations (0.5 to 50 μg/ml) of EcEE alone or in combination with BPA or DOX. Cell viability was analyzed through resazurin assay. Gene transcription levels for NCL, FOS, p21, AURKA and bcl-xl were determined through qRT-PCR. Cytological analysis included evaluation of nuclear and mitotic anomalies after DAPI staining, immunodetection of histone H3 lysine 9 acetylation (H3K9ac) and assessment of DNA damage by TUNEL assay.ResultsSevere loss of HT29 cell viability was detected for 50 μg/ml EcEE immediately after 24 h exposure whereas the lower concentrations assayed (0.5, 5 and 25 μg/ml) resulted in significant viability decreases after 96 h. Exposure to 25 μg/ml EcEE for 48 h resulted in irreversible cell damage leading to a drastic decrease in cell viability after 72 h recovery in EcEE-free medium. 48 h 25 μg/ml EcEE treatment also induced alteration of colony morphology, H3K9 hyperacetylation, transcriptional up regulation of p21 and down regulation of NCL, FOS and AURKA, indicating reduced proliferation capacity. This treatment also resulted in drastic mitotic and nuclear disruption accompanied by up-regulation of bcl-xl, limited TUNEL labeling and nuclear size increase, suggestive of a non-apoptocic cell death pathway. EcEE/BPA co-exposure increased mitotic anomalies particularly for the lowest EcEE concentration, although without major effects on viability. Conversely, EcEE/DOX co-exposure decreased cell viability in relation to DOX for all EcEE concentrations, without affecting the DOX-induced cell cycle arrest.ConclusionsEcEE has cytotoxic activity on HT29 cancer cells leading to mitotic disruption and non-apoptotic cell death without severe induction of DNA damage. Interaction experiments showed that EcEE can increase BPA aneugenic effects and EcEE synergistic effects with DOX supporting a potential use as adjuvant in chemotherapeutic approaches.

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Section: Discussionmentioning

confidence: 99%

“…Eupatoriopicrin has been associated with induction DNA damage in Ehrlich ascites tumour [12] as well as with cytostatic activity and both in vitro and in vivo tumour growth inhibition properties in Lewis lung carcinoma and FIG 26 fibrosarcoma [13]. …”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Eupatorium cannabinum L. ethanolic extract against colon cancer cells and interactions with Bisphenol A and Doxorubicin

Ribeiro

Ressurreição

Viegas

et al. 2014

BMC Complement Altern Med

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“…Eucalyptus species possessed anticancer effects against wide range of cell lines including human colon cancer cell lines HCT116, RKO, human ECV-304 cell lines, WEHI-3, HT-29, HL-60 cell lines, MCF-7, Hep-2, HepG-2, HeLa, HCT-116 , Caco-2 cell lines, liver , lung, prostate and breastcell lines (349)(350)(351)(352)(353)(354)(355)(356)(357)(358) . Eupatorium cannabinum showed antiproliferative effect against colon cancer cellline HT29, leukaemia, ZNS tumor cells (V 251), DLD-1, CCRF-CEM, HL-60 cell lines, Jurkat cellline and Ehrlich ascites tumour cells (358)(359)(360)(361)(362)(363)(364)(365)(366) . Euphorbia hirta showed cytotoxicity in brine shrimp test and possessed anticancer effect against human epidermoid carcinoma KB 3-1 cells (367)(368)(369) .…”

Section: Plants With Anticancer Effectsmentioning

confidence: 99%

Natural sources as promising future anticancer therapies - A review

Thuwaini¹

2022

GSC Biol. and Pharm. Sci.

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“…Les molécules actives sont des sesquiterpènes lactoniques de l'extrait aqueux : eupatoriopicrine, eupatolide et 5'-déhydroxy-eupatriopicrine. Ceux-ci agissent sur différentes cellules tumorales : cellules KB (nasopharynx), cellules HeLa (col utérin), cellules carcinomateuses GLC4 pulmonaires, cellules de la lignée EAT d'Ehrlich, cellules leucémiques P388, cellules FIO 26 (fibrocarcinome), cellules L5178Y(s) lymphomateuses, cellules de la lignée normale humaine RK13 [15].…”

Section: Les Plantes Immunostimulantes Et La Thérapeutique Antitumoraleunclassified

Les plantes immunostimulantes adjuvantes de la th�rapeutique antitumorale*

Goetz¹

2004

Phytother

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Dans cet article, l'auteur examine si les plantes immunostimulantes peuvent jouer un rôle dans la thérapie des états cancéreux. Les plantes immunostimulantes à effet global sont des plantes agissant par leurs polysaccharides immuno-actifs. Celles-ci ont aussi des activités anticanreuses spécifiques selon des expérimentations sur l'animal liées à des constituants différents. Par ailleurs ces mêmes plantes sont souvent aussi adaptogènes. Il est possible de conclure que l'utilisation en paralèlle d'un traitement anticancéreux conventionnel semble indiquée. Abstract:In this paper the author tries to examine the immuno-stimulating plants which may have a role in cancer therapy. Polysaccharides containing immuno-stimulating plants also contain active principles with specific effects in various cancers. Most of them are adaptogenic plants too. It is possible to conclude that they can be used in parallel with a conventional anti-cancer treatment.

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Induction of DNA damage in Ehrlich ascites tumour cells by exposure to eupatoriopicrin

Cited by 9 publications

References 15 publications

Cytotoxicity of Eupatorium cannabinum L. ethanolic extract against colon cancer cells and interactions with Bisphenol A and Doxorubicin

Cytotoxicity of Eupatorium cannabinum L. ethanolic extract against colon cancer cells and interactions with Bisphenol A and Doxorubicin

Natural sources as promising future anticancer therapies - A review

Les plantes immunostimulantes adjuvantes de la th�rapeutique antitumorale*

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