Induction of diabetes by Streptozotocin in rats

Akbarzadeh, Azim; Norouzian, Dariush; Mehrabi, Mohammad Reza; Jamshidi, Shirin; Farhangi, Ali Akbar; Verdi, A. Allah; Mofidian, Seyed Mohammad Ali; Rad, Behzad

doi:10.1007/bf02913315

Cited by 435 publications

(346 citation statements)

References 8 publications

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“…Moreover, some nuclei showed fragmented chromatin and dissolution. These findings were in accordance with Akbarzadeh et al (17) who reported that STZ injection caused β cell damage in rat pancreas. CD34 antibody was used to immunostain hematopoietic stem cells.…”

Section: Discussionsupporting

confidence: 93%

The Effect of In Vivo Mobilization of Bone Marrow Stem Cells on the Pancreas of Diabetic Albino Rats (A Histological & Immunohistochemical Study)

Ismail¹,

Kamel²,

Yacoub³

et al. 2013

Int J Stem Cells

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Background and Objectives: The rapidly increasing number of diabetic patients across the world drew the attention to develop more effective therapeutic approaches. Recent investigations on newly differentiated insulin producing cells (IPCs) revealed that they could be derived from embryonic, adult mesenchymal and hematopoietic stem cells. This work was planned to evaluate the role of StemEnhance (Aphanizomenon flos-aquae [AFA] plant extract) in mobilizing naturally occurring bone marrow stem cells as well as in improving streptozotocin-induced diabetic rats. Methods and Results: Twenty adult male albino rats were divided into four groups namely the control, the diabetic, the positive control-StemEnhance and the diabetic-StemEnhance groups. After diabetes induction by streptozotocin (STZ), rats received StemEnhance for four weeks. The mean number of blood CD34 immunopositive cells was measured by flowcytometry and random blood sugar was measured weekly. The pancreas was removed from the sacrificed rats and processed for staining with H&E and immunohistochemical staining for CD34+ve and insulin +ve cells. CD34+ve cells increased in the blood after introduction of StemEnhance. CD34+ve cells were observed in the pancreas and the insulin producing cells in the islets of Langerhans were increased from the second to the fourth week of treatment. Blood glucose level improved but it was still higher than the control level after four weeks of StemEnhance treatment. Conclusions: This work points to the significant role of StemEnhance in stem cell mobilization and the improvement of diabetes mellitus.

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Section: Discussionsupporting

confidence: 93%

The Effect of In Vivo Mobilization of Bone Marrow Stem Cells on the Pancreas of Diabetic Albino Rats (A Histological & Immunohistochemical Study)

Ismail¹,

Kamel²,

Yacoub³

et al. 2013

Int J Stem Cells

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“…Challenge with STZ begin an autoimmune process that results in the destruction of langerhans islets b-cells with emergence of clinical diabetes within 2-4 days, and that is why this animal model have been used for primary screening of test drugs for antidiabetic activity [10]. We found that intoxication with STZ caused a significant increase in diabetogenic and dyslipidemia parameters; namely blood glucose, HbA 1C , LPO, FFA and sd-LDL lipid and protein components of plasma lipoproteins, as well as adipose and liver.…”

Section: Discussionmentioning

confidence: 99%

“…A group of six animals in a cage were kept in controlled conditions, temperature 25-26°C, relative humidity 50-60 % and 12/12 h light/dark cycle (light from 08:00 AM-08:00 PM) and provided with standard pellet diet(Dayal Industries, Barabanki, UP, India) and water adlibitum. Diabetes was induced by intraperitoneal injection of streptozotocin (65 mg/kg b.w) in animals [10].After 3 days of injection, diabetes was confirmed by glucometer. Rats with blood glucose level 300-350 mg/dl were included in the study.…”

Section: Antidyslipidemic Activity In Streptozotocin Induced Diabeticmentioning

confidence: 99%

Antidyslipidemic Effect of Ocimum sanctum Leaf Extract in Streptozotocin Induced Diabetic Rats

et al. 2014

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The antidyslipidemic activity of Ocimum sanctum leaf extract was studied in streptozotocin induced diabetic rats. In this model, there was significant increase in plasma markers of diabetic-dyslipidemia following diminution of lipid metabolizing enzymes. Oral administration of leaf extract (500 mg/kg b.w.p.o.) for 15 days resulted in significant decrease in diabetogenic and dyslipidemia parameters; namely blood glucose, glycosylated hemoglobin, lipid peroxide, free fatty acids, small dense low density lipoprotein, lipid and protein components of plasma lipoproteins, adipose and liver. The regulation of lipids was accompanied by stimulation of postheparin lipolytic activity, reactivation of lecithin cholesterol acyl transferase and hepatic lipoprotein lipase enzymes. The results of the present study demonstrated antidyslipidemic and antioxidant activities in leaf extract of O. sanctum which could be used in prevention of diabeticdyslipidemia and related complications.

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“…The rats were given ad libitum access to food and water. Before induction of diabetes, fasting blood glucose was monitored and ranged between 135±5 mg/dl (Akbarzadeh et al, 2007). 24-hour urine samples were collected from all rats included in the control group.…”

Section: Methodsmentioning

confidence: 99%

“…In the literature, it is recognized that this drug induces experimental diabetes in three days by destruction of pancreatic β-cells (Akbarzadeh et al, 2007). In this study, we used a dose of 60 mg/ kg that was administered intravenously.…”

Section: Induction Of Diabetesmentioning

confidence: 99%

Evaluation of Urinary Tryptophan Metabolite Levels in Non-diabetic Compared to Diabetic Rats

Olar¹,

Ştefan²,

Cățioiu³

et al. 2017

BUASVMCN-VM

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Diabetes mellitus is one of the most common metabolic disorders in animals. Thus, currently, it is imperative to introduce non-invasive, economical and rapid methods for the investigation of diabetes in animals. In this study, the urine samples collected from 10 non-diabetic and 10 streptozotocin-induced diabetic rats were investigated by the spectrofluorimetric technique. Emission spectra for the urine samples were obtained following an excitation wavelength of 280 and 400 nm. The investigated fluorophores were mainly tryptophan metabolites, and significant differences resulted between the mean heights of the emission bands attributed to these fluorophore compounds in diabetic compared to non-diabetic rats. The shape of the spectral windings after the utilization of these two excitation wavelengths was almost similar for diabetic and non-diabetic rats; however, there were some discriminatory elements between the two types of investigated samples. In conclusion, the obtained urine fluorescence spectra allow a clear differentiation between diabetic and non-diabetic rats.

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Induction of diabetes by Streptozotocin in rats

Abstract: The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult

Cited by 435 publications

References 8 publications

The Effect of In Vivo Mobilization of Bone Marrow Stem Cells on the Pancreas of Diabetic Albino Rats (A Histological & Immunohistochemical Study)

The Effect of In Vivo Mobilization of Bone Marrow Stem Cells on the Pancreas of Diabetic Albino Rats (A Histological & Immunohistochemical Study)

Antidyslipidemic Effect of Ocimum sanctum Leaf Extract in Streptozotocin Induced Diabetic Rats

Evaluation of Urinary Tryptophan Metabolite Levels in Non-diabetic Compared to Diabetic Rats

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