Evaluation of Urinary Tryptophan Metabolite Levels in Non-diabetic Compared to Diabetic Rats

Abstract

Diabetes mellitus is one of the most common metabolic disorders in animals. Thus, currently, it is imperative to introduce non-invasive, economical and rapid methods for the investigation of diabetes in animals. In this study, the urine samples collected from 10 non-diabetic and 10 streptozotocin-induced diabetic rats were investigated by the spectrofluorimetric technique. Emission spectra for the urine samples were obtained following an excitation wavelength of 280 and 400 nm. The investigated fluorophores were mainly tryptophan metabolites, and significant differences resulted between the mean heights of the emission bands attributed to these fluorophore compounds in diabetic compared to non-diabetic rats. The shape of the spectral windings after the utilization of these two excitation wavelengths was almost similar for diabetic and non-diabetic rats; however, there were some discriminatory elements between the two types of investigated samples. In conclusion, the obtained urine fluorescence spectra allow a clear differentiation between diabetic and non-diabetic rats.

CaCO3-based carriers with prolonged release properties for antifungal drug delivery to hair follicles

“…Most peaks from a total of 10 that were present in natural urine (without Gf delivery) were identified according to information on known fluorophores published elsewhere. 88,89 Typical control urine spectra with fitted peaks and the resulting approximation curve are presented in Fig. SI 16 (see ESI†).…”

Paraclinical Investigations in the Pathology of the Urinary System in Dogs

“…In these patients, one of the exams performed was spectrofluorimetric analysis ( Figure 5). For the interpretation of the results, in the literature, an attribution of the emission bands obtained was first performed, followed by the Gaussian fitting of the spectra obtained (Olar et al,2017;Olar et al 2018).This technique can identify the urinary fluororophores whose concentration can significantly increase in diabetic comparatively with non-diabetic animals (Olar et al, 2017).…”