Induction of cytotoxic T-lymphocytes specific for bovine herpesvirus-1 by DNA immunization

Deshpande, Muralidhar; Ambagala, Thanuja; Hegde, Nagendra R.; Hariharan, Mangala J.; Navaratnam, Manjula; Srikumaran, Subramaniam

doi:10.1016/s0264-410x(02)00375-4

Cited by 30 publications

(20 citation statements)

References 40 publications

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“…Recently, several groups have demonstrated the ability of suicidal DNA vaccines to induce higher level humoral and cell-mediated immunity against a variety of antigens, and the immunized animals developed more pronounced immune response than those received a conventional DNA vaccine encoding the same antigen [18][19][20][21]. In addition, a most recent report has showed that suicidal DNA vaccines could break immunological tolerance by activating innate antiviral pathways, whereas the convention DNA vaccines encoding the same antigen could not [22].…”

Section: Introductionmentioning

confidence: 99%

Enhanced immunogenicity to food-and-mouth disease virus in mice vaccination with alphaviral replicon-based DNA vaccine expressing the capsid precursor polypeptide (P1)

Xiao

Fang

et al. 2006

Virus Genes

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Recently, alphavirus replicon-based DNA vaccines, also known as suicidal DNA vaccines, have emerged as an important strategy to enhance the potency of DNA vaccines. In this study, two different types of DNA vaccines encoding the capsid precursor polypeptide (P1) of foot-and-mouth disease virus (FMDV) were constructed and the immunogenicity were investigated and compared in mouse model. The first DNA vaccine, pcDP1, is a conventional plasmid DNA vaccine in which P1 was driven directly by a cytomegalovirus promoter. The second DNA vaccine, pSCAP1, is a Semliki Forest virus (SFV) replicon-based DNA vaccine encoding the same antigen. In vitro expression and characterization indicated that two vaccine vectors could correctly produce the P1 antigen. However, pSCAP1 could induce obvious apoptosis of the transfected cells. After immunization in BALB/c mice, the P1-specific ELISA antibodies, neutralizing antibodies, as well as lymphocyte proliferative responses induced by pSCAP1 were significantly higher than those obtained in mice immunized with pcDP1. Notably, mice immunized with the pSCAP1 had the determined ability of clearing virus in their sera after FMDV challenge. These results indicate that the SFV replicon-based DNA vaccine pSCAP1 are more effective than conventional DNA vaccine and it can be considered a promising approach for the development of a safety and efficacious vaccine against FMDV.

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Section: Introductionmentioning

confidence: 99%

Enhanced immunogenicity to food-and-mouth disease virus in mice vaccination with alphaviral replicon-based DNA vaccine expressing the capsid precursor polypeptide (P1)

Xiao

Fang

et al. 2006

Virus Genes

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“…Se determinó que el 0.6 ± 0.7% (3/480) de los animales presentaron anticuerpos contra el BHV-1. Las tres vacas serorreactoras fueron Holstein, mayores de 6 años de edad y pertenecieron a un solo hato, y presentaron títulos de anticuerpos de 1:64 (2) y 1:32 (1). Los resultados indicaron que la enfermedad se encuentra poco difundida entre los animales de la zona en estudio, por lo que se deberá evitar el ingreso de animales infectados.…”

Section: Introductionunclassified

Seroprevalencia Del Virus De La Rinotraqueitis Infecciosa Bovina en Bovinos De Crianza Extensiva Enla Zona De Cajamarca.

et al. 2012

Rev. investig. vet. Perú

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The prevalence of the bovine herpesvirus 1 (BHV-1) was studied in cattle raised under an extensive rearing system and without history of vaccination. Herds were located in the province of San Pablo, department of Cajamarca, Peru. Blood samples from 480 bovine were collected for detection of antibodies against BHV-1 by viral neutralization test. The seroprevalence was 0.6 ± 0.7% (3/480). The three positive Holstein cows, older than 6 years, were part of the same herd and the antibody titers were 1:64 (2) and 1:32 (1). The results indicated that the BHV-1 is now widely distributed in cattle of the studied region.

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“…Several groups have demonstrated the ability of suicidal DNA vaccines to induce high-level humoral and cellmediated immunity against a variety of antigens, with immunized animals developing more prominent immune responses than those receiving a conventional DNA vaccine encoding the same antigen (Berglund et al, 1998;Deshpande et al, 2002;Hariharan et al, 1998;Kirman et al, 2003;Leitner et al, 2000). In addition, it has been demonstrated that suicidal DNA vaccines could break immunological tolerance by activating innate antiviral pathways, in contrast to conventional DNA vaccines encoding the same antigen (Leitner et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The RNA selfamplifying property is of considerable interest with respect to vaccine biosafety: the vector replicates at the RNA level and not at the DNA level, so the rate of foreign DNA present in vivo and possessing 'genome integration potential' is controlled and does not increase following vaccination (contrary to some attenuated or recombinant vaccines). Furthermore, when a suicidal DNA vaccine is transfected into cells, it leads eventually to apoptosis of the transfected cells (Kohno et al, 1998;Leitner et al, 2000), which is particularly important in alleviating the concerns of potential integration and cell transformation generated by the use of conventional DNA vaccines (Gurunathan et al, 2000;Lewis & Babiuk, 1999).Several groups have demonstrated the ability of suicidal DNA vaccines to induce high-level humoral and cellmediated immunity against a variety of antigens, with immunized animals developing more prominent immune responses than those receiving a conventional DNA vaccine encoding the same antigen (Berglund et al, 1998;Deshpande et al, 2002;Hariharan et al, 1998;Kirman et al, 2003;Leitner et al, 2000). In addition, it has been demonstrated that suicidal DNA vaccines could break immunological tolerance by activating innate antiviral pathways, in contrast to conventional DNA vaccines encoding the same antigen (Leitner et al, 2003).…”

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confidence: 99%

Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

Sun

Liu

Guo

et al. 2007

Journal of General Virology

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A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against swine vesicular disease virus (SVDV). The 1BCD gene of SVDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/1BCD, was transfected into BHK-21 cells and the antigenicity of the expressed protein was confirmed using an indirect immunofluorescence assay. Immunogenicity was studied in guinea pigs and swine. Animals were injected intramuscularly three times with pSCA/1BCD at regular intervals. Anti-SVDV antibodies were detected by ELISA, the lymphocyte proliferation response was tested by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide method and neutralizing antibodies were measured by microneutralization tests. The data showed that SVDV-specific antibodies, neutralizing antibodies and lymphocyte proliferation were induced in both guinea pigs and swine. Furthermore, after three successive vaccinations with pSCA/1BCD, half of the pigs were protected against challenge with SVDV. These results should encourage further work towards the development of a DNA vaccine against SVDV. INTRODUCTIONSwine vesicular disease (SVD) is a highly contagious viral pig disease, characterized by the appearance of vesicles on the coronary bands, heels of the feet and less commonly on the snout and tongue. Due to the similarity of these lesions to those caused by foot-and mouth-disease (FMD), SVD is subject to international controls and is listed by the World Organization for Animal Health. SVD was first identified in Italy in 1966(Nardelli et al., 1968 and several more outbreaks have been reported subsequently in Europe and eastern Asia (Brocchi et al., 1997). However, in the recent past, reports of SVD have been limited to Portugal and Italy.SVD vaccines have been developed previously to control the disease, both in monovalent form (Gourreau et al., 1975) and in combination with FMD (Mitev et al., 1978), and an SVD subunit vaccine has also been described, although it was not very efficacious (Jiménez-Clavero et al., 1998). Although the inactivated virus vaccines are effective in protecting against clinical signs, there has been little, if any, assessment made of their ability to reduce wild-type virus transmission and no effective vaccine is available commercially. Once introduced, SVD could be a difficult disease to eradicate and improved methods of control would be highly beneficial, including the development of safer and more effective vaccines to protect and control this disease.Suicidal DNA vaccines, based on the alphavirus replicon, have emerged as an important strategy to enhance immunogenicity and to improve the biosafety of conventional DNA vaccines (Berglund et al., 1998;Leitner et al., 1999;Lundstrom, 2000). Unlike the conventional DNA vaccine construct in which heterologous gene expression is driven directly by the RNA polymerase II-dependent promoter, suicidal DNA vaccines based on the replicon of alphaviruses, including Sindbis viru...

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Induction of cytotoxic T-lymphocytes specific for bovine herpesvirus-1 by DNA immunization

Cited by 30 publications

References 40 publications

Enhanced immunogenicity to food-and-mouth disease virus in mice vaccination with alphaviral replicon-based DNA vaccine expressing the capsid precursor polypeptide (P1)

Enhanced immunogenicity to food-and-mouth disease virus in mice vaccination with alphaviral replicon-based DNA vaccine expressing the capsid precursor polypeptide (P1)

Seroprevalencia Del Virus De La Rinotraqueitis Infecciosa Bovina en Bovinos De Crianza Extensiva Enla Zona De Cajamarca.

Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

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