1996
DOI: 10.1242/jcs.109.8.2023
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Induction of constitutive melanogenesis in amelanotic mouse melanoma cells by transfection of the human melanocortin-1 receptor gene

Abstract: The human melanocortin-1 (MC1) receptor was stably expressed in the amelanotic mouse melanoma cell clone B16-G4F which does not express its own (mouse) MC1 receptor and hence is unresponsive to alpha melanocyte stimulating hormone (alpha MSH). From several stable transfectant cell lines expressing the human MC1 receptor in relatively high numbers, three melanin producing clones (G4F-12, 14, and 15) and one amelanotic clone (G4F-7) were further analyzed in competition binding experiments and in cAMP and melanin… Show more

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Cited by 26 publications
(2 citation statements)
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“…The melanin production on bare plastic and unmodified multilayers is due to a low constitutive activity of the MC1 receptor. 17 To verify that the decreasing response of the cells with CP1/2 (CP1 or CP2) position in the film, in the cAMP assay, was not due to degradation of the multilayers, we preincubated, for 12 h, different PLL-CP1 containing multilayers with cell supernatants. These supernatants were obtained from B16-F1 cells cultivated for 2 h in serum free MEM containing 0.2% BSA, the same culture conditions as those used for cAMP assay.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The melanin production on bare plastic and unmodified multilayers is due to a low constitutive activity of the MC1 receptor. 17 To verify that the decreasing response of the cells with CP1/2 (CP1 or CP2) position in the film, in the cAMP assay, was not due to degradation of the multilayers, we preincubated, for 12 h, different PLL-CP1 containing multilayers with cell supernatants. These supernatants were obtained from B16-F1 cells cultivated for 2 h in serum free MEM containing 0.2% BSA, the same culture conditions as those used for cAMP assay.…”
Section: Resultsmentioning
confidence: 99%
“…The experiments were done in the absence of phosphodiesterase inhibitors. 17 AFM Imaging. AFM height mode images of PEI(PSS/ PAH) 2 PGA/PLL-CP1(PGA/PLL) 2 multilayers on a silica surface were taken by using a Nanoscope III (Digital Instruments, Santa Barbara, CA) equipped with a silicon nitride cantilever (model MLCT-AUHW Park Scientific, Sunnyvale, CA) having a spring constant of 0.03 N/m.…”
Section: Chemicalsmentioning
confidence: 99%