Induction of Apoptosis with<i>Kigelia africana</i>fruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway

Guon, Tae-Eun; Chung, Ha Sook

doi:10.20307/nps.2016.22.3.209

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Erythrina alkaloids: erythraline, erysodine, erysotrine, 8oxoerythraline, and 11-methoxyerysodine [133] Cytotoxicity with LC 50 value > 240 μg/ml [134]. Seed oil suppressed human colon adenocarcinoma (Caco-2) and human embryonic kidney (HEK-293) cell growth in a dose-dependent manner [140] An 80% methanol extract of the roots exhibited cytotoxicity to brine shrimps with LC 50 of 7.2 μg/ml [138] Fruit extracts increased the sub-G1 phase (apoptosis) population in HCT116 human colon cancer cells [141] Markhamia lutea (Benth.) K. Schum Cycloartane triterpenoids, musambins A-C and their 3-Oxyloside derivatives musambiosides A-C [142], Oleanolic acid, pomolic acid, 2-epi-tormentic acid, musambin A, and b-sitosterol-3-O-b-D-glucopyranoside [143,144] Anticancer activity against Ehrlich ascites carcinoma cells with an IC 50 value of 27.0 μg/mL [143].…”

Section: Anticancer Plants Used In Local Communities Of Ugandamentioning

confidence: 99%

Medicinal Plants Used in Traditional Management of Cancer in Uganda: A Review of Ethnobotanical Surveys, Phytochemistry, and Anticancer Studies

Omara

Kiprop

Ramkat

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The burden of neoplastic diseases is a significant global health challenge accounting for thousands of deaths. In Uganda, about 32,617 cancer cases were reported in 2018, accompanied by 21,829 deaths. In a view to identify some potential anticancer plant candidates for possible drug development, the current study was designed to compile the inventory of plants with reported anticancer activity used in rural Uganda and the evidences supporting their use in cancer therapy. An electronic survey in multidisciplinary databases revealed that 29 plant species belonging to 28 genera distributed among 24 families have been reported to be used in the management of cancer in Uganda. Anticancer plants were majorly from the families Bignoniaceae (7%), Caricaceae (7%), Fabaceae (7%), Moraceae (7%), and Rutaceae (7%). Most species occur in the wild (52%), though some are cultivated (48%). The growth habit of the plants is as trees (55%) or herbs (45%). Anticancer extracts are usually prepared from leaves (29%), bark (24%), roots (21%), and fruits (13%) through decoctions (53%), as food spices (23%) or pounded to produce ointments that are applied topically (10%). Prunus africana (Hook.f.) Kalkman, Opuntia species, Albizia coriaria (Welw. ex Oliver), Daucus carota L., Cyperus alatus (Nees) F. Muell., Markhamia lutea (Benth.) K. Schum., and Oxalis corniculata L. were the most frequently encountered species. As per global reports, Allium sativum L., Annona muricata L., Carica papaya L., Moringa oleifera Lam., Opuntia species, Prunus africana (Hook.f.) Kalkman, and Catharanthus roseus (L.) G. Don. are the most studied species, with the latter having vincristine and vinblastine anticancer drugs developed from it. Prostate, cervical, breast, and skin cancers are the top traditionally treated malignancies. There is a need to isolate and evaluate the anticancer potential of the bioactive compounds in the unstudied claimed plants, such as Cyperus alatus (Nees) F. Muell., Ficus dawei Hutch., Ficus natalensis Hochst., and Lovoa trichilioides Harms, and elucidate their mechanism of anticancer activity.

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Section: Anticancer Plants Used In Local Communities Of Ugandamentioning

confidence: 99%

Medicinal Plants Used in Traditional Management of Cancer in Uganda: A Review of Ethnobotanical Surveys, Phytochemistry, and Anticancer Studies

Omara

Kiprop

Ramkat

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…These results correlated with the previous study that artonin E showed anti-proliferation and apoptosis induction by induced cleaved caspase-7 and cleaved-PARP activation in A431 skin cancer cells, resulting in apoptosis cell death [ 26 ]. In general, there are two types of caspases, initiator and effector caspase; the caspase cascade, caspase-9 (initiator caspase) can activate caspase-7 (effector caspase), which then cleaves vital substrates, such as PARP that plays an important role in DNA repairing [ 30 ]. These present results related to previous research demonstrating that artonin E induced apoptosis in several cancer cell types including MDA-MB231, MCF-7, SKOV-3, H460, and A431 [ 11 , 15 , 24 , 27 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Artonin E on Cell Growth Inhibition and Apoptosis Induction in Colon Cancer LoVo and HCT116 Cells

et al. 2022

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Today, colon cancer is the leading cause of cancer death. In Thailand, colon cancer is the third most common cancer in men and the second in women. Currently, the treatments for colon cancer include surgery, chemotherapy, radiation therapy, immunotherapy, hormone therapy, targeted drug therapy, and stem cell therapy. However, some treatments have side effects for cancer patients, causing unwanted symptoms. In addition, targeted therapy comes with a high cost for patients. Therefore, bioactive compounds might be a good choice for colon cancer treatment. In this study, we investigated the effect of artonin E on apoptosis induction in colon cancer LoVo and HCT116 cells. The concentration ranges of artonin E at 3, 5, 10, and 30 µg/mL in LoVo cells and 1, 1.5, 2, and 3 µg/mL in HCT116 cells were examined. The results implied that artonin E decreased cell viability and increased apoptotic cells in a dose-dependent manner. In addition, artonin E stimulated mitochondrial membrane potential (ΔΨm) changes associated with apoptosis by increasing the sub-G1 population analyzed by flow cytometry. Western blotting showed that artonin E increased the proapoptotic protein, Bax, and decreased anti-apoptotic proteins’ (Bcl-2 and Bcl-x) expression. Moreover, artonin E also increased cleaved caspase-7 and cleaved-PARP expression in both LoVo and HCT116 cells. Interestingly, artonin E induced apoptosis through p-ERK1/2, p-p38/p38, and p-c-Jun expression in both cells. Our results suggested that artonin E induced apoptosis via caspase activation associated with the MAPKs signaling pathway. Therefore, artonin E might be used as a potential anticancer drug for colon cancer in the future.

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“…Cancer is a lethal disease typically caused by mutations that result in uncontrolled cell growth that subsequently invades surrounding tissues; such systemic metastasis is now the second leading cause of death worldwide [1,2]. Natural products from many species (including microorganisms, marine organisms, and other plants) have proved valuable resources for securing molecular treatments for cancer [3][4][5][6][7][8][9][10][11][12]. Indeed, many anti-cancer drugs derived from natural products (such as paclitaxel, doxorubicin, and rapamycin) are currently either in clinical use or preclinical evaluation to treat cancer patients [4,5,13].…”

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confidence: 99%

Raphanus sativus Sprout Causes Selective Cytotoxic Effect on p53-Deficient Human Lung Cancer Cells in vitro

Baeka

Rohb

Choi

et al. 2017

Natural Product Communications

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Raphanus sativus L. (Brassicaceae) is widely consumed in many different forms worldwide. Its sprouts, in particular, are commonly consumed as a health food. R. sativus sprout has recently been shown to have anti-tumor activity on human colon cancer cells, suggesting that it may have potential use in cancer prevention and treatment. The extent of this anti-tumor activity and its underlying mechanisms, however, remain to be investigated in other types of cancer cells. In this study, we showed that the MeOH extract from R. sativus sprout exhibits significant but variable cytotoxic effects on human lung adenocarcinoma cells depending on their p53 status. The MeOH extract decreased the viability of p53-deleted human lung cancer cells (H1299 and Calu-6) by inducing apoptosis; this effect, however, did not occur for wild-type p53 cancer cells (A549), for cells expressing a p53 mutant lacking the C terminus (H1264), or for non-tumor fibroblast cells (NIH3T3). Phytochemical analyses of the MeOH extract allowed us to identify and isolate β-sitosterol as a major component of the MeOH extract. Direct treatment with β-sitosterol significantly reduced the viability of Calu-6 cells, suggesting that it may, in part, contribute to R. sativus sprout's anti-tumor activity. This work provides experimental evidence for a novel biological application of R. sativus sprout in treating human lung cancer, and it identifies the main component involved in this effect, further supporting its potential use as a functional food for cancer management.

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Induction of Apoptosis withKigelia africanafruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway

Cited by 6 publications

References 25 publications

Medicinal Plants Used in Traditional Management of Cancer in Uganda: A Review of Ethnobotanical Surveys, Phytochemistry, and Anticancer Studies

Medicinal Plants Used in Traditional Management of Cancer in Uganda: A Review of Ethnobotanical Surveys, Phytochemistry, and Anticancer Studies

Effects of Artonin E on Cell Growth Inhibition and Apoptosis Induction in Colon Cancer LoVo and HCT116 Cells

Raphanus sativus Sprout Causes Selective Cytotoxic Effect on p53-Deficient Human Lung Cancer Cells in vitro

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