Inducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)

Barpete, Surendra; Bakhsh, Allah; Anayol, Emine; Özcan, Sancar Fatih; Oğuz, Muhammet Çağrı; Karakoç, Ömer Cem; Özcan, Sebahattin

doi:10.3906/biy-1509-16

Cited by 4 publications

(2 citation statements)

References 32 publications

(32 reference statements)

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“…A 25 g powder of the Nutrient broth-Merck Germany (20 g/l, dissolved in 1 H O, 37°C after autoclaving). pTF101AoPR1AcBar plant expression vector containing the cry1Ac gene under the control of the woundinduced (AoPR1) promoter [34,Fig. 1].…”

Section: Inoculation and Transformationmentioning

confidence: 99%

“…The insect bioassays were checked following Barpete et al [34] using transgenic and nontransgenic seeds of the cultivars placed in separate glass Petri dishes at 24 ± 1°C and 70% moisture in the laboratory together with ten males and females in each Petri dish. The experiments were cultured at 25 ± 1 ℃ and relative humidity of 70 ± 2% under day temperature conditions.…”

Section: Insect Bioassaysmentioning

confidence: 99%

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Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

Kahriz

Khawar

2022

Preprint

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Background Seed weevils are one of the causes of post harvest rice yield losses in Turkey. There are many cultural and chemical methods to control these insects.PurposeThis study aimed to control these using Agrobacterium tumefaciens mediated genetic transformation in two rice cultivars namely Hamzadere and Osmancı-97.Methods and results Two days old germinated seeds of the two cultivars with 2-3 mm long shoots were used as explants. They were treated with Agrobacterium tumefciens containing a pTF101AoPR1AcBar plant expression vector containing the cry1Ac gene under the control of the wound-induced (AoPR1) promoter. The transformation event was genotype-dependent. Out of 3000 and 2600 explants of cv Hamzadere and cv Osmancık-97, only 92 (3.07%) and 32 (1.230%) plants were selected on MS medium containing 3.0 and 2.5 mg/l bialaphos. These were validated using PCR, bioassay, and ELISA tests. All plants were held in the greenhouse until T0 and T1 seed settings. three (0.10%) plants of cv Hamzadere and 5 (0.19%) plants of cv. Osmancık 97 could be validated through all tests in T₀ and T₁ generations in both cultivars of rice for the first time using a novel and a very simple transformation procedure.ConclusionThese results are very encouraging and will influence future rice transformation techniques and improve rice breeding technologies.

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Section: Inoculation and Transformationmentioning

confidence: 99%

Section: Insect Bioassaysmentioning

confidence: 99%

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

Kahriz

Khawar

2022

Preprint

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An Efficient Agrobacterium-Mediated Genetic Transformation Using Embryonic Axis in Cotton (Gossypium hirsutum L.)

Ahmed

Barpete

Uranbey

et al. 2020

Russ J Plant Physiol

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Apprehending the potential of BABY BOOM transcription factors to mitigate cotton regeneration and transformation

2020

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Since the advent of transgenic technology, the incorporation of gene(s) encoding traits of economic importance in cotton is being practiced worldwide. However, factors like recalcitrant nature of cotton cultivars, in vitro regeneration via tissue culture (especially via somatic embryogenesis), genotype dependency, long and toilsome protocols impede the pace of development of transgenic cotton. Besides that, types and age of explants, media composition, plant growth regulators and other environmental factors affect in vitro cotton regeneration significantly. The studies of genetic control of in vitro regeneration in plants have elucidated the role of certain transcription factor genes that are induced and expressed during somatic embryogenesis. Among these transcription factors, BABY BOOM (BBM) plays a very important role in signal transduction pathway, leading to cell differentiation and somatic embryos formation. The role of BBM has been established in plant cell proliferation, growth and development even without exogenous growth regulators. This review intends to provide an informative summary of regeneration and transformation problems in cotton and the latest developments in utilization of BBM transcription factors in cotton. We believe that the use of BBM will not only ease cotton genetic improvement but will also accelerate cotton breeding programmes.

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Inducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)

Abstract: To date, there are few reports on the signaling and metabolic pathways of carbohydrates during cell culture (Huang and Liu, 2002;Huang et al., 2006). In addition to serving as an energy source, sucrose acts as a hypotonic and hypertonic osmotic agent in tissue

Cited by 4 publications

References 32 publications

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

An Efficient Agrobacterium-Mediated Genetic Transformation Using Embryonic Axis in Cotton (Gossypium hirsutum L.)

Apprehending the potential of BABY BOOM transcription factors to mitigate cotton regeneration and transformation

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