Inducible protein in rat hepatomas with expression alternative to alpha‐fetoprotein

Eraiser, T. L.; Yazova, A. K.; Полторанина, В. С.; Kuprina, N. I.; Karamova, E.R.; Shipova, L. Ya.; Лазаревич, Н. Л.; Абелев, Г. И.

doi:10.1002/(sici)1097-0215(19980130)75:3<371::aid-ijc8>3.0.co;2-#

Cited by 3 publications

(1 citation statement)

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“…To investigate the antitumoral efficacy of the hybrid vector carrying mIL-12, orthotopic hepatocellular carcinomas were established in Buffalo rats by implantation of rat McA-RH7777 cells into the liver. This model was chosen because McA-RH7777 cells have been reported to express AFP (28). Our data showed that animals receiving HC-Ad-AFP-mIL-12 had a reduced tumor size (Fig.…”

Section: Resultsmentioning

confidence: 76%

Increased Efficacy and Safety in the Treatment of Experimental Liver Cancer with a Novel Adenovirus-Alphavirus Hybrid Vector

et al. 2006

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An improved viral vector for cancer gene therapy should be capable of infecting tumors with high efficiency, inducing specific and high-level expression of transgene in the tumor and selectively destroying tumor cells. In the design of such a vector to treat hepatocellular carcinoma, we took advantage of (a) the high infectivity of adenoviruses for hepatic cells, (b) the high level of protein expression and proapoptotic properties that characterize Semliki Forest virus (SFV) replicon, and (c) tumor selectivity provided by A-fetoprotein (AFP) promoter. We constructed a hybrid viral vector composed of a helper-dependent adenovirus containing an SFV replicon under the transcriptional control of AFP promoter and a transgene driven by SFV subgenomic promoter. Hybrid vectors containing murine interleukin-12 (mIL-12) genes or reporter gene LacZ showed very specific and high-level expression of transgenes in AFP-expressing hepatocellular carcinoma cells, both in vitro and in an in vivo hepatocellular carcinoma animal model. Infected hepatocellular carcinoma cells were selectively eliminated due to the induction of apoptosis by SFV replication. In a rat orthotopic liver tumor model, treatment of established tumors with a hybrid vector carrying mIL-12 gene resulted in strong antitumoral activity without accompanying toxicity. This new type of hybrid vectors may provide a potent and safe tool for cancer gene therapy.

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Section: Resultsmentioning

confidence: 76%

Increased Efficacy and Safety in the Treatment of Experimental Liver Cancer with a Novel Adenovirus-Alphavirus Hybrid Vector

et al. 2006

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Cellular aspects of alpha-fetoprotein reexpression in tumors

Абелев

Eraiser

1999

Seminars in Cancer Biology

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From whole body to cellular models of hepatic triglyceride metabolism: man has got to know his limitations

Green

Pramfalk

Morten

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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The liver is a main metabolic organ in the human body and carries out a vital role in lipid metabolism. Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases, encompassing a spectrum of conditions from simple fatty liver (hepatic steatosis) through to cirrhosis. Although obesity is a known risk factor for hepatic steatosis, it remains unclear what factor(s) is/are responsible for the primary event leading to retention of intrahepatocellular fat. Studying hepatic processes and the etiology and progression of disease in vivo in humans is challenging, not least as NAFLD may take years to develop. We present here a review of experimental models and approaches that have been used to assess liver triglyceride metabolism and discuss their usefulness in helping to understand the aetiology and development of NAFLD.

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Inducible protein in rat hepatomas with expression alternative to alpha‐fetoprotein

Cited by 3 publications

References 23 publications

Increased Efficacy and Safety in the Treatment of Experimental Liver Cancer with a Novel Adenovirus-Alphavirus Hybrid Vector

Increased Efficacy and Safety in the Treatment of Experimental Liver Cancer with a Novel Adenovirus-Alphavirus Hybrid Vector

Cellular aspects of alpha-fetoprotein reexpression in tumors

From whole body to cellular models of hepatic triglyceride metabolism: man has got to know his limitations

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