Cellular aspects of alpha-fetoprotein reexpression in tumors

Абелев, Г. И.; Eraiser, T. L.

doi:10.1006/scbi.1998.0084

Cited by 99 publications

(69 citation statements)

References 41 publications

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“…In addition to the overexpression of ␣-fetal protein, also found among most HCC patients in the present study, dozens of gene͞EST markers reminiscent of embryonization or dedifferentiation of hepatocytes were noted. Although most of those features may reflect the consequences of the liver cell transformation and thus are unlikely to play an essential role in carcinogenesis, some of them may be used as clinical diagnostic markers, especially when combined with the proteomics analysis (17). An interesting finding was that many enzymes for biotransformation were down-regulated, leaving the question open of whether this phenomena simply represents an altered liver function or whether it could be involved in the HCC pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver

Huang

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

310

240

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Human hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. In this work, we report on a comprehensive characterization of gene expression profiles of hepatitis B viruspositive HCC through the generation of a large set of 5-read expressed sequence tag (EST) clusters (11,065 in total) from HCC and noncancerous liver samples, which then were applied to a cDNA microarray system containing 12,393 genes͞ESTs and to comparison with a public database. The commercial cDNA microarray, which contains 1,176 known genes related to oncogenesis, was used also for profiling gene expression. Integrated data from the above approaches identified 2,253 genes͞ESTs as candidates with differential expression. A number of genes related to oncogenesis and hepatic function͞differentiation were selected for further semiquantitative reverse transcriptase-PCR analysis in 29 paired HCC͞noncancerous liver samples. Many genes involved in cell cycle regulation such as cyclins, cyclin-dependent kinases, and cell cycle negative regulators were deregulated in most patients with HCC. Aberrant expression of the Wnt-␤-catenin pathway and enzymes for DNA replication also could contribute to the pathogenesis of HCC. The alteration of transcription levels was noted in a large number of genes implicated in metabolism, whereas a profile change of others might represent a status of dedifferentiation of the malignant hepatocytes, both considered as potential markers of diagnostic value. Notably, the altered transcriptome profiles in HCC could be correlated to a number of chromosome regions with amplification or loss of heterozygosity, providing one of the underlying causes of the transcription anomaly of HCC.

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Section: Discussionmentioning

confidence: 99%

Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver

Huang

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

310

240

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“…AFP, produced by the yolk sac and liver, is expressed at high levels during early development in fetus and decreases to a low or undetected level in adults (Abelev and Eraiser, 1999). However, AFP expression is reactivated in liver neoplastic transformation.…”

Section: Introductionmentioning

confidence: 99%

Targeted hepatocellular carcinoma proapoptotic BikDD gene therapy

Dai

Yeh

et al. 2010

Oncogene

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“…These cells are thought to have both clonogenic and bipotential capacity, i.e., the ability to proliferate and differentiate into cells of either hepatocyte or biliary epithelial cells [8] . There is also evidence that under certain conditions, oval cells can be induced to differentiate into non-hepatic lineages including intestinal and pancreatic epithelium [9] . The origin of oval cells and their precise location within the liver have remained enigmatic [10] .…”

Section: Introductionmentioning

confidence: 99%