Induced mutagenesis in Jatropha curcas L. using gamma rays and detection of DNA polymorphism through RAPD marker

Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation

“…The loss of the bands may be related to single and double-strand breaks, modified bases, oxidized bases, and point mutations induced by gamma radiation 31 . The appearance of new bands may indicate changes in some oligonucleotide priming sites due to mutations or conformational DNA changes 32 . Ionizing radiation induces cytotoxic and mutagenic effects which are due to the genetic damage which may be caused during or immediately after transfer of radiation energy to genomic DNA certain characteristics as, (1) the damage by shortlived reactive oxygen species (ROS) and (OH -) generated by the radiolysis of water; (2) the damage due interaction of DNA with radiation; (3) mutations are set on genome replication 8 .…”

Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay

“…High levels of DNA polymorphism observed by RAPD in the present study may be due to heritable changes in the nucleotide sequences of treated DNA samples in coding and non-coding regions (Lal et al, 2011) or to structural changes in the genomic DNA that alter the distance between two annealing sites and delete an existing site of new one or to changes during gene expression (Dhakshanamoorthy et al, 2011). Conversely, the deletion or insertion of the amplified DNA regions, and changes in the nitrogenous base sequence that alter primer binding sites may lead to high levels of polymorphisms in the RAPD profile (Dhakshanamoorthy et al, 2011).…”

“…Conversely, the deletion or insertion of the amplified DNA regions, and changes in the nitrogenous base sequence that alter primer binding sites may lead to high levels of polymorphisms in the RAPD profile (Dhakshanamoorthy et al, 2011). Therefore, DNA polymorphisms are considered to be a suitable method for measuring patterns of genetic variation in response to CO 2 and O 3 treatments.…”

Gamma Ray Induced Cytomorphological Variations in <i>Sesbania cannabina</i> Poir.

“…The higher exposures are usually inhibitors of seed germination in angiosperms and gymnosperms (Dhakshanamoorthy et al 2011, Akhaury and Singh 1993, Majeed and Muhammad 2010. The impact of gamma radiation on S. cannabina and its tolerance levels to it manifested itself in terms of germination and survival percentages, which decreased with increasing doses; results similar to the present investigation were also reported by Tah (2006).…”