Induced expression of defense-related genes in<i>Arabidopsis</i>upon infection with<i>Phytophthora capsici</i>

Wang, Yan; Bouwmeester, Klaas; Mortel, Judith E. van de; Shan, Weixing; Govers, F.

doi:10.4161/psb.24618

Cited by 7 publications

(5 citation statements)

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“…Plants employ several mechanisms to protect themselves against various pathogen infections, which include the activation of defense-related genes, resulting in the synthesis of various antimicrobial substances (27). Most of the defense-related genes are induced by the activation of different signaling molecules such as salicylic acid (SA), jasmonic acid (JA), or ethylene (ET) to govern antimicrobial activity via hydrolysis of fungal cells, synthesis of toxic substances, and stimulation of antioxidants (28).…”

Section: Discussionmentioning

confidence: 99%

Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress

Mishra

Singh

Mahfooz

et al. 2018

Appl Environ Microbiol

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Endophytes have been explored and found to perform an important role in plant health. However, their effects on the host physiological function and disease management remain elusive. The present study aimed to assess the potential effects of endophytes, singly as well as in combination, in (L.) Dunal, on various physiological parameters and systemic defense mechanisms against Seeds primed with the endophytic bacteria and individually and in combination demonstrated an enhanced vigor index and germination rate. Interestingly, plants treated with the two-microbe combination showed the lowest plant mortality rate (28%) under stress. Physiological profiling of treated plants showed improved photosynthesis, respiration, transpiration, and stomatal conductance under pathogenic stress. Additionally, these endophytes not only augmented defense enzymes and antioxidant activity in treated plants but also enhanced the expression of salicylic acid- and jasmonic acid-responsive genes in the stressed plants. Reductions in reactive oxygen species (ROS) and reactive nitrogen species (RNS) along with enhanced callose deposition in host plant leaves corroborated well with the above findings. Altogether, the study provides novel insights into the underlying mechanisms behind the tripartite interaction of endophyte-- and underscores their ability to boost plant health under pathogen stress. is well known for producing several medicinally important secondary metabolites. These secondary metabolites are required by various pharmaceutical sectors to produce life-saving drugs. However, the cultivation of faces severe challenge from leaf spot disease caused by To keep pace with the rising demand for this plant and considering its capacity for cultivation under field conditions, the present study was undertaken to develop approaches to enhance production of through intervention using endophytes. Application of bacterial endophytes not only suppresses the pathogenicity of but also mitigates excessive ROS/RNS generation via enhanced physiological processes and antioxidant machinery. Expression profiling of plant defense-related genes further validates the efficacy of bacterial endophytes against leaf spot disease.

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Section: Discussionmentioning

confidence: 99%

Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress

Mishra

Singh

Mahfooz

et al. 2018

Appl Environ Microbiol

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“…Generally, SA signalling is essential to resist infection from biotrophic pathogens, whereas jasmonic acid/ethylene (JA/ET) signalling is necessary for inhibiting infections from necrotrophic pathogens (Dodds & Rathjen, 2010). Interestingly, several reports have shown that both SA and JA/ET signalling are required to resist infection by Phytophthora pathogens in Arabidopsis and N. benthamiana (Attard et al, 2010;Shibata et al, 2010;Wang et al, 2013). Analysis of the expression of PR1 and PDF1.2, marker genes for SA and JA/ET, respectively, showed that PR1 was upregulated quickly but PDF1.2 was downregulated in Col-0 in response to P. parasitica.…”

Section: Rtp1-1 Plants Display Broad Spectrum Resistance To Biotrophimentioning

confidence: 99%

“…PR1 transcripts accumulate faster in rtp1-1 plants PR1 and PDF1.2 are key marker genes for salicylic acid (SA) and jasmonic acid (JA), respectively (Uknes et al, 1993;Yun et al, 2003). Previous research has shown that Phytophthora infection induces the expression of PR1 and PDF1.2 (Attard et al, 2010;Wang et al, 2013). Strong induction of PR1 was observed following P. parasitica infection in both Col-0 and rtp1-1 plants.…”

Section: Generation Of Omentioning

confidence: 99%

“…It has been hypothesized that NADPH oxidase function could lead to SA accumulation and in this context, the expression of PR proteins have been shown to be partly dependent on the ROS accumulation in response to P. parasitica in tobacco (Wi et al, 2012). Furthermore, PR1 has been shown to play an important role in plant defence against P. capsici (Wang et al, 2013). In addition, PR1 also participates in root resistance of Arabidopsis against P. parasitica (Attard et al, 2010).…”

Section: Rtp1 Negatively Regulate Ros and Cell Death In Plant Immunitymentioning

confidence: 99%

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RTP1 encodes a novel endoplasmic reticulum (ER)‐localized protein in Arabidopsis and negatively regulates resistance against biotrophic pathogens

et al. 2015

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SummaryOomycete pathogens cause serious damage to a wide spectrum of plants. Although host pathogen recognition via pathogen effectors and cognate plant resistance proteins is well established, the genetic basis of host factors that mediate plant susceptibility to oomycete pathogens is relatively unexplored.Here, we report on RTP1, a nodulin-related MtN21 family gene in Arabidopsis that mediates susceptibility to Phytophthora parasitica.RTP1 was identified by screening a T-DNA insertion mutant population and encoded an endoplasmic reticulum (ER)-localized protein. Overexpression of RTP1 rendered Arabidopsis more susceptible, whereas RNA silencing of RTP1 led to enhanced resistance to P. parasitica. Moreover, an RTP1 mutant, rtp1-1, displayed localized cell death, increased reactive oxygen species (ROS) production and accelerated PR1 expression, compared to the wild-type Col-0, in response to P. parasitica infection. rtp1-1 showed a similar disease response to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000, including increased disease resistance, cell death and ROS production. Furthermore, rpt1-1 exhibited resistance to the fungal pathogen Golovinomyces cichoracearum, but not to the necrotrophic pathogen Botrytis cinerea.Taken together, these results suggest that RTP1 negatively regulates plant resistance to biotrophic pathogens, possibly by regulating ROS production, cell death progression and PR1 expression.

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“…To infect Arabidopsis, 5 mL of zoospore suspensions (100 zoospores in total) were dripped on both side of midrib. Inoculated leaves were maintained at 25°C in the dark with high humidity for 36 h. Inoculated leaves were photographed under UV light and relative quantification of P. capsici biomass was performed to evaluate infection severity as described (Wang et al, 2013). For B. cinerea inoculation, B05.10 used in this study was grown on potato dextrose agar (PDA) at 25°C in the dark.…”

Section: Pathogen Inoculation On Arabidopsismentioning

confidence: 99%

A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway

Hou

Zhou

et al. 2022

Front. Plant Sci.

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Xanthomonas campestris pv. campestris (Xcc)-induced black rot is one of the most serious diseases in cruciferous plants. Using beneficial microbes to control this disease is promising. In our preliminary work, we isolated a bacterial strain (JR48) from a vegetable field. Here, we confirmed the plant-growth-promoting (PGP) effects of JR48 in planta, and identified JR48 as a Priestia megaterium strain. We found that JR48 was able to induce plant resistance to Xcc and prime plant defense responses including hydrogen peroxide (H2O2) accumulation and callose deposition with elevated expression of defense-related genes. Further, JR48 promoted lignin biosynthesis and raised accumulation of frees salicylic acid (SA) as well as expression of pathogenesis-related (PR) genes. Finally, we confirmed that JR48-induced plant resistance and defense responses requires SA signaling pathway. Together, our results revealed that JR48 promotes plant growth and induces plant resistance to the crucifer black rot probably through reinforcing SA accumulation and response, highlighting its potential as a novel biocontrol agent in the future.

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Induced expression of defense-related genes inArabidopsisupon infection withPhytophthora capsici

Cited by 7 publications

References 8 publications

Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress

Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress

RTP1 encodes a novel endoplasmic reticulum (ER)‐localized protein in Arabidopsis and negatively regulates resistance against biotrophic pathogens

A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway

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