A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway

Abstract

Xanthomonas campestris pv. campestris (Xcc)-induced black rot is one of the most serious diseases in cruciferous plants. Using beneficial microbes to control this disease is promising. In our preliminary work, we isolated a bacterial strain (JR48) from a vegetable field. Here, we confirmed the plant-growth-promoting (PGP) effects of JR48 in planta, and identified JR48 as a Priestia megaterium strain. We found that JR48 was able to induce plant resistance to Xcc and prime plant defense responses including hydrogen peroxide (H2O2) accumulation and callose deposition with elevated expression of defense-related genes. Further, JR48 promoted lignin biosynthesis and raised accumulation of frees salicylic acid (SA) as well as expression of pathogenesis-related (PR) genes. Finally, we confirmed that JR48-induced plant resistance and defense responses requires SA signaling pathway. Together, our results revealed that JR48 promotes plant growth and induces plant resistance to the crucifer black rot probably through reinforcing SA accumulation and response, highlighting its potential as a novel biocontrol agent in the future.

Novel Bacillus and Prestia isolates from Dwarf century plant enhance crop yield and salinity tolerance

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Identification of culturable bacteria associated with the rhizosphere of Lablab purpureus growing in Namibia

“…Priestia megaterium was described to increase plant disease resistance and exhibits promise as a future biocontrol agent (Li et al, 2022). Since they were able to demonstrate that this bacterium produces ACC deaminase, has nitrogen-xing capabilities, secretes Indole Acetic Acid (IAA), and can solubilize both potassium and phosphate, the same authors claimed that it is a bacteria that promotes plant growth.…”

Reaction of Commercial Cultivars of Kiwifruit to Infection by Root-knot Nematode and Its Biocontrol Using Endophytic Bacteria

“…The use of nematode-resistant cultivars along with biological control in integrated management programs provides a suitable strategy to control RKN ( Mukhtar et al, 2014 ; Mukhtar, 2018 ; Eliwa and Hagag, 2021 ). Resistant cultivars have been successfully used against RKN in tomato ( Lizardo et al, 2022 ), soybean ( Izuogu et al, 2015 ), pepper ( Bello et al, 2015 ) and even provided the complete-spectrum resistance in the case of Myrobalan plum ( Claverie et al, 2011 ). Using biological control agents against plant pathogens, especially plant parasitic nematodes where resistant cultivars are not available or in combination with partially resistant cultivars and other control strategies provides effective and durable control against nematodes.…”

“…It has been shown that Priestia megaterium strain JR48 induces resistance against Xanthomonas campestris pv. campestris in cruciferous plants by induction of hydrogen peroxide accumulation, callose deposition, and elevated expression of defense-related genes, especially pathogenesis-related ( PR ) genes through the salicylic acid signaling pathway ( Li et al, 2022 ). Application of Streptomyces sp.…”