2020
DOI: 10.1016/j.celrep.2020.107670
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Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array

Abstract: Highlights d Microfluidic droplet pairs sequentially trapped in capillary anchors before merging d 1 spheroid/droplet, with microenvironment modulations driven by droplet merging d A wide range of drug concentrations tested on hepatic-like spheroids in a single chip d Data-driven approach unravels 3D tissue-level dynamic drug response Authors Raphaë l F.

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Cited by 83 publications
(129 citation statements)
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“…Programmable DNA gels could also find applications in single-cell sequencing (where gel beads and droplet microfluidics are already routine) for instance to extract from a cellular the transcripts belonging to a specific cellular type [ 9 ]. In tissue engineering, DNA gel beads could support complex engineering of spheroids [ 86 ], for instance to spatially organize multicellular spheroid, to select spheroid with given phenotypes, or to massively screen culture conditions [ 87 , 88 ]. Lastly, it could also find application at the intersection of regenerative medicine and drug delivery.…”
Section: Discussionmentioning
confidence: 99%
“…Programmable DNA gels could also find applications in single-cell sequencing (where gel beads and droplet microfluidics are already routine) for instance to extract from a cellular the transcripts belonging to a specific cellular type [ 9 ]. In tissue engineering, DNA gel beads could support complex engineering of spheroids [ 86 ], for instance to spatially organize multicellular spheroid, to select spheroid with given phenotypes, or to massively screen culture conditions [ 87 , 88 ]. Lastly, it could also find application at the intersection of regenerative medicine and drug delivery.…”
Section: Discussionmentioning
confidence: 99%
“…The microfluidic chips were fabricated using the protocols detailed in previous studies. [17,21,25] Briefly, the chip consisted of a top part made with poly(dimethylsiloxane) (PDMS, SYLGARD 184, Dow Corning, 1:10 w/w ratio of curing agent to bulk material) on which was imprinted a flow focusing device connected to an emulsification channel (serpentine), diverging rails, and terminated by a culture chamber; the bottom part of the chip was a PDMS layer (about 400 µm thick) etched with an array of capillary traps, which was bonded on a microscopy glass slide ( Figure 1A). The top and bottom parts of the chip were assembled after plasma treatment (Harrick, Ithaca, USA).…”
Section: Microfabricationmentioning
confidence: 99%
“…This data-driven approach allowed the heterotopic 3D cell culturing behaviour to be monitored and linked single-cell measurements with population measurements. 100 This platform yielded time-resolved, single-cell data, revealing a dynamic response regulated at the spheroid level. This was achieved by introducing a new asymmetric design for the anchors, which led to a qualitative transformation in the functionality of the microfluidic approach for a range of biological applications, including tissue engineering, models of immuno-therapies, and understanding host-pathogen interactions.…”
Section: Aqueous Two-phase System (Atps)mentioning
confidence: 99%