Advanced biomedical applications based on emerging 3D cell culturing platforms

Wang, Anheng; Madden, Leigh A.; Paunov, Vesselin N.

doi:10.1039/d0tb01658f

Cited by 32 publications

(24 citation statements)

References 118 publications

(164 reference statements)

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“…In the water-in-water emulsion method, cells are injected into two different phases of an aqueous solution; an osmotic effect then triggers aggregation ( Figure 2 D ). This method is cost-efficient and enables high-throughput production 51 , 52 . More recently, 3D printing technology has emerged as a novel fabrication strategy; this is reproducible, allows high-throughput production, and is precise.…”

Section: Spheroid Fabrication Methodsmentioning

confidence: 99%

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The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

et al. 2021

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A spheroid is a complex, spherical cellular aggregate supporting cell-cell and cell-matrix interactions in an environment that mimics the real-world situation. In terms of tissue engineering, spheroids are important building blocks that replace two-dimensional cell cultures. Spheroids replicate tissue physiological activities. The use of spheroids with/without scaffolds yields structures that engage in desired activities and replicate the complicated geometry of three-dimensional tissues. In this mini-review, we describe conventional and novel methods by which scaffold-free and scaffolded spheroids may be fabricated and discuss their applications in tissue regeneration and future perspectives.

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Section: Spheroid Fabrication Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

et al. 2021

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“…Whereas the generation of sufficient numbers of scaffold-free spheroids in a reproducible quality was initially challenging, this limitation has been successfully addressed [6] , [12] . Current scaffold-free 3D cell cultures address many of the above-mentioned constraints of scaffold-based approaches, such as limited biocompatibility and potential approval for clinical use.…”

Section: Introductionmentioning

confidence: 99%

Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome

Souza²,

Dengjel

2021

Matrix Biology Plus

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The human skin has a highly developed extracellular matrix (ECM) that is vital for proper skin functioning, its 3D architecture playing a pivotal role in support and guidance of resident and invading cells. To establish relevant in vitro models mimicking the complex design observed in vivo, scaffold-based and scaffold-free 3D cell culture systems have been developed. Here we show that scaffold-free systems are well suited for the analysis of ECM protein regulation. Using quantitative mass spectrometry-based proteomics in combination with magnetic 3D bioprinting we characterize changes in the proteome of skin fibroblasts and squamous cell carcinoma cells. Transferring cells from 2D to 3D without any additional scaffold induces a profound upregulation of matrisome proteins indicating the generation of a complex, tissue-like ECM.

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“…Therefore, utilizing olegels to secure bioavailable substitutes for PHOs is seriously restricted in the food industry. Another possible routine for developing substitutes for PHOs is the water-in-water emulsion template [24][25][26]. The shortcoming of such a system is the flavor and commercial acceptance.…”

Section: Introductionmentioning

confidence: 99%

Novel Pickering High Internal Phase Emulsion Stabilized by Food Waste-Hen Egg Chalaza

Wang

2021

Foods

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A massive amount of chalaza with nearly 400 metric tons is produced annually as waste in the liquid-egg industry. The present study aimed to look for ways to utilize chalaza as a natural emulsifier for high internal phase emulsions (HIPEs) at the optimal production conditions to expand the utilization of such abundant material. To the author’s knowledge, for the first time, we report the usage of hen egg chalaza particles as particulate emulsifiers for Pickering (HIPEs) development. The chalaza particles with partial wettability were fabricated at different pH or ionic strengths by freeze-drying. The surface electricity of the chalaza particles was neutralized when the pH was adjusted to 4, where the chalaza contained a particle size around 1500 nm and held the best capability to stabilize the emulsions. Similarly, the chalaza reaches proper electrical charging (−6 mv) and size (700 nm) after the ionic strength was modified to 0.6 M. Following the characterization of chalaza particles, we successfully generated stable Pickering HIPEs with up to 86% internal phase at proper particle concentrations (0.5–2%). The emulsion contained significant stability against coalescence and flocculation during long term storage due to the electrical hindrance raised by the chalaza particles which absorbed on the oil–water interfaces. Different rheological models were tested on the formed HIPEs, indicating the outstanding stability of such emulsions. Concomitantly, a percolating 3D-network was formed in the Pickering HIPES stabilized by chalaza which provided the emulsions with viscoelastic and self-standing features. Moreover, the current study provides an attractive strategy to convert liquid oils to viscoelastic soft solids without artificial trans fats.

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Advanced biomedical applications based on emerging 3D cell culturing platforms

Abstract: We discuss the recent progress in the development of scaffold-free techniques for the fabrication of cell spheroids and their biomedical applications.

Cited by 32 publications

References 118 publications

The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

The utility of biomedical scaffolds laden with spheroids in various tissue engineering applications

Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome

Novel Pickering High Internal Phase Emulsion Stabilized by Food Waste-Hen Egg Chalaza

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