communication and help regulate the function on the scale of whole organs and organism. The different types of molecules are present at different concentrations in vivo and act over a range of time scales, from seconds to days. Indeed, variations in the rate of secretion of different molecules indicate the state of the cells, for example, as a response to changes in their environment. Standard methods for measuring secreted molecules in typical cell culture experiments, however, require the presence of a large number of molecules, which translates into volumes of tens to hundreds of µL and give information at the level of large populations of cells. As a result, these techniques hide the heterogeneities that may exist on the scale of individual cells. It is becoming increasingly clear, however, that these heterogeneities are present and play a determining role in many biological processes, such as the immune response to an unknown pathogen, [1] or as a prognostic marker for some cancer types. [2] 2002303 (2 of 11)