Indigenous entomopathogenic fungi as potential biological control agents of rose sawfly, Arge rosae L. (Hymenoptera: Argidae)

Abstract

The rose sawfly, Arge rosae L. (Hymenoptera: Argidae), is one of the most destructive pests of roses grown both indoors and outdoors in Antalya province (south-western part of Turkey). Especially in greenhouse rose cultivation, growers depend heavily on synthetic pesticides to control this pest along with other arthropod pests in their crops. The aim of this study was to evaluate biocontrol potential of some indigenous soil-borne entomopathogenic fungi (EPF) against the pest. In pathogenicity assays, a total of 17 EPF isolates, belonging to three species [Beauveria bassiana (Bals.) Vuill. -14, Clonostachys rosea (Link) Schroers -2 and Isaria farinosa (Holmsk.) Fr. -1] were tested against the 4 th instar larvae of A. rosae under laboratory conditions. All the isolates were applied at a conidial suspension of 1 × 10 7 conidia/ml, using the spray method. The results from the assays showed that bioefficacy of tested isolates increased significantly with elapsed time up to 9 days after inoculation. Of the 17 isolates tested, 5 isolates of B. bassiana (BbDm-1, BbKp-1, BbMp-1, BbSr-1 and BbMg-2) and 1 isolate of I. farinosa (IfGp-1) appeared to be most promising, causing mortalities between 76.7 and 86.7% 3 days post treatment. Probit analysis of mortality data showed that there were significant differences in the susceptibility of 4 th instar larvae of A. rosae to tested EPF isolates. The above-mentioned six isolates had the lowest LT50 and LT95 values (ranged from 1.66 to 7.50 and 4.81 to 8.49 days, respectively), implying their high virulence and their biocontrol potential against the pest. Phylogenetic analysis based on the ITS region sequence revealed that all tested isolates showed high similarities (ranged from 99 to 100%) with other isolates of their respective fungal species in GenBank.Overall results suggested that the most virulent above-mentioned six isolates had significant potential as biological control agents against A. rosae.

New parasitoid records of Arge rosae (Linnaeus, 1758) (Hymenoptera: Argidae) from Diyarbakır province: Tetrastichus hylotomarum (Bouché, 1834) (Hymenoptera: Eulophidae) and Boethus thoracicus (Giraud, 1872) (Hymenoptera: Ichneumonidae)

“…Arge rosae (Linnaeus, 1758) (Hymenoptera: Argidae) (Figure 2) Distribution in world: Bulgaria (Margina et al, 1999), Persia (Sahragard and Heydari, 2001;Sharifi et al, 2012;Khosravi et al, 2015) Distribution in Türkiye: Antalya, Diyarbakır, Isparta, İstanbul, İzmir, Manisa (Zümreoğlu, 1972;Oğurlu et al, 1996;Özbek and Çalmaşur, 2005;Demirözer and Karaca, 2011;Bolu et al, 2021;Baki et al, 2021). Host plants: Rosa canina Linnaeus, 1753, R. damascena Miller (Margina et al, 1999;Özbek and Çalmaşur, 2005;Bolu et al, 2021).…”

“…Material examined: 10♂♂, 10♀♀ (multiple samples), Locality: Diyarbakır (Sur district), 37°53'31.8"N, 40°16'22.2"E, 666 m. As a result; it was determined that the NC of turfgrass can be estimated with similar prediction performance using both hand-held NDVI meter and hand-held chromameter in a more objective and economic way. Arge rosae is one of the pests (Figure 1) which causes economic damage to roses (Sharifi et al, 2012;Bolu et al, 2021;Baki et al, 2021). This damage occurs in two ways.…”

“…In literature, there are many studies on control of the this pest species in the world as well as in our country. In particular, studies are focused on biological and biotechnical methods to leave residues on nature (Baki et al, 2021). In this purpose, the aim of this study was to determine the natural enemies of the pest.…”

Potential of entomopathogens in biological control of the box-tree moth, Cydalima perspectalis (Lepidoptera: Crambidae)

“…There are commercially available fungal biopesticides in a safe alternative control against chemical use. Most insects are highly susceptible to fungi infections (Lovett and Leger 2017, Litwin et al 2020, Baki et al 2021. According to the literature, a list including the entomopathogenic fungi of C. perspectalis as agents for natural control of this pest is given in the Table . mosorosea conidia and fungus growth.…”

Potential of entomopathogenic fungi as biological control agents of Yponomeuta malinellus Zeller, 1838 (Lepidoptera: Yponomeutidae)

“…'Starking') foliage under controlled conditions (25 ± 2°C, 60 ± 5 RH, and 16:8 h L: D photoperiod) in a climate room. They were used in the experiments when all of them reached the 4 th instar larval stage (Baki et al 2021).…”