Increasing Liquid Chromatography–Tandem Mass Spectrometry Throughput by Mass Tagging: A Sample-Multiplexed High-Throughput Assay for 25-Hydroxyvitamin D2 and D3

Netzel, Brian C.; Cradic, Kendall W.; Bro, Eric T.; Girtman, Adam B.; Cyr, Richard C.; Singh, Ravinder J.; Grebe, Stefan K.

doi:10.1373/clinchem.2010.157115

Cited by 55 publications

(25 citation statements)

References 21 publications

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“…In order to achieve such multiplexing of samples, each sample needs to become chemically modified in a different way, allowing unique identification by the MS instrument. Using this approach, a five-fold increased sample throughput has been achieved in the measurement of 25(OH)D in human serum [81]. When combining some of the above mentioned approaches, sample throughput can be increased even further.…”

Section: Sample Throughputmentioning

confidence: 99%

The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

Ouweland

Kema²

2012

Journal of Chromatography B

129

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Section: Sample Throughputmentioning

confidence: 99%

The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

Ouweland

Kema²

2012

Journal of Chromatography B

129

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“…However, it is still difficult to quantify the low endogenous levels of 1,25(OH) 2 VitD in small sample volumes. In an attempt to further increase the method sensitivity, new effective dienophiles, 4-(4′-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione; 4-[2-(3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxo-2-quinoxalinyl)ethyl]-3H-1,2,4-triazole-3,5(4H)-dione; and other "triazolinediones," have been developed (25,(33)(34)(35)(36)(37). Among them, the positively charged derivatization reagent, Amplifex-Diene and SecoSET, are reported to have a 10-fold ioniziation efficiency improvement over PTAD (25,36,38).…”

mentioning

confidence: 99%

“…However, the immunoextraction method was developed only for 1,25(OH) 2 VitD and did not include other metabolites, 25(OH)VitD and 24R,25(OH) 2 VitD. These metabolites are also important to quantify, because the 24R,25(OH) 2 VitD has proved to be a critical component for healing processes in tissues and bones, and knowing the levels of both 25(OH)VitD and 24R,25(OH) 2 VitD is useful for epidemiological studies (35). In addition, immunoextraction required a relatively large sample volume (400-500 µl) compared with other methods.…”

mentioning

confidence: 99%

A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine

Wan

Yang

Barnych

et al. 2017

Journal of Lipid Research

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This article is available online at http://www.jlr.orgIn 1943, vitamin D was recommended as an essential nutrient for the US public. People can acquire the precursors to the bioactive forms, vitamin D 3 and vitamin D 2 , from diet, supplements, and sunlight. In the liver, both forms of vitamin D are rapidly hydroxylated to corresponding 25-hydroxyvitamin D [25(OH)VitD]. Being a predominant metabolite, circulating 25(OH)VitD serves as the preferred surrogate biomarker of vitamin D nutritional status (1, 2). Low levels of 25(OH)VitD indicate low vitamin D nutritional intake, which is related to many diseases, such as osteomalacia, multiple sclerosis, Parkinson's disease, asthma, and other health risks, including muscle weakness, diabetes, and cognitive decline or Alzheimer's disease (3-7). Consequently, analytical techniques for measurement of 25(OH)VitD have been developed (8)(9)(10)(11)(12). In the kidney, 25(OH)VitD is further hydroxylated to 1,25-dihydroxyvitamin D [1,25(OH) 2 VitD]. 1,25(OH) 2 VitD is the major biologically active metabolite and plays an important role in decreasing the risk of many diseases, including common cancers, cardiovascular disease, autoimmune disease, and chronic kidney disease (13)(14)(15)(16)(17). Most recently, we have demonstrated that 1,25(OH) 2 VitD also appears to be important for the health of the eye (18). The influence that these biologically active forms of vitamin D have on these numerous diseases and associated organ systems 23 January 2017. Published, JLR Papers in Press, February 1, 2017 DOI 10.1194 26,26,27,27,(OH)VitD 3 ; ALTM, all laboratory trimmed mean; CUDA, 12-(3-cyclohexyl-ureido)-dodecanoic acid; DEQAS, Vitamin D External Quality Assessment Scheme; LLE-SPE, liquid-liquid extraction followed by solid-phase extraction; LLOQ, lower limit of quantification; MRM, multiple reaction monitoring; PTAD, 4-phenyl-1,2,4-triazole-3,5-dione; PyrNO, 2-nitrosopyridine; QC, quality control. This work was supported by the National Institutes of Health/National Eye Institute grant R01 EY021747 (to M.A.W.), National Institute of Environmental Health Sciences (NIEHS) grant R01 ES002710, NIEHS Superfund Research Program grant P42 ES004699 (to B.D.H.), the West Coast Metabolomics Center at UC Davis (National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases grant U24 DK097154), and National Institutes of Health/NIEHS grant 1K99ES024806-01 (to K.S.S.L.). Manuscript received 22 November 2016 and in revised form

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“…In 2011, a group at Mayo Clinic developed and implemented an elegant mul tiplexing method where up to 5 patient samples are multiplexed within one single LC-MS/MS injection, using the specifically designed mass tags. The throug hput that can be achieved with this methodology is up to 300 specimens per hour or 7200 spe ci mens per instrument per day, matching the through put of automated immunoassays (37).…”

Section: Choice Of Methodsmentioning

confidence: 94%

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

Babić¹

2012

Journal of Medical Biochemistry

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Summary: Demand for vitamin D testing has been on a constant rise worldwide, partially due to mounting evidence linking vitamin D status to overall health and well-being. Currently available assays measure 25-hydroxy vitamin D (25-OHD), a major circulating form of vitamin D. Available methodologies include immunoassays and mass spectrometry based methods (LC-MS/MS). Until recently, the only immunoassays available for diagnostic use in the US have been DiaSorin radioimmunoassay (RIA) and an automated immunoassay on a LIAISON ® platform. Within the last year, Siemens and Abbott successfully launched immunoassays for determination of total vitamin D on their respective automated platforms, Centaur ® and ARCHITECT ® . Development of robust and precise Vitamin D immunoassays has historically been plagued with difficulty. One of the major challenges is development of specific antibodies against such a small antigen. Vitamin D is also highly hydrophobic molecule predominantly bound to vitamin D binding protein (DBP). It is likely, therefore, that immunoassays might be affected to varying extent by the DBP concentration. Adoption of LC-MS/MS into clinical laboratories has enabled development of accurate and almost fully automated methods that could handle increasing volume demands, especially in large volume reference laboratories. Smaller to mid-size hospital laboratories as well as physician offices have neither funds nor technical expertise to implement LC-MS/MS based testing. Our laboratory at the University of Chicago Medical Center has also seen increase in vitamin D volume and currently performs close to 20,000 25-OHD assays per year. We have recently developed an LC-MS/MS method for quantitation of 25-OHD 2 (obtained from plant sources) and 25-OHD 3 (endogenous and animal sources). Prior to acquisition of LC-MS/MS Kratak sadr`aj: Broj zahteva za odre|ivanjem vitamina D je u konstantnom porastu {irom sveta, delom zbog sve vi{e dokaza koji povezuju status vitamina D sa op{tim zdravljem. Trenutno raspolo`ivim testovima odre|uje se 25-hidroksi vitamin D (25-OHD), glavni oblik vitamina D u cirkulaciji. Po stoje}e metodologije uklju~uju imunometrijska odre |i vanja i tehnike zasnovane na masenoj spektrometriji (LC-MS/MS). Do nedavno, jedine raspolo`ive imunometrijske metode kori{}ene za dijagnostiku u SAD su bile DiaSorin radioimunoodre|ivanje (RIA) i automatizovano imuno odre|ivanje na LIAISON ® platformi. U toku pro{le godine Siemens i Abbott su uspe{no lansirali imunometrijske testove za odre|ivanje ukupnog vitamina D na svojim odgova raju}im automatizovanim platformama, Centaur ® i ARCHI-TECT ® . Razvoj robusnih i preciznih imunometrijskih testova za odre|ivanje vitamina D su, istorijski gledano, pratili problemi. Jedan od najve}ih izazova je razvoj specifi~nih antitela protiv malog antigena. Vitamin D je tako|e jako hidrofoban molekul predominantno vezan za vitamin D vezu ju}i protein (DBP). Stoga postoji verovatno}a da na imuno odre|ivanja u razli~itom stepenu mo`e da uti~e koncentracija DBP. Uvo|enje LC-MS/MS u...

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Increasing Liquid Chromatography–Tandem Mass Spectrometry Throughput by Mass Tagging: A Sample-Multiplexed High-Throughput Assay for 25-Hydroxyvitamin D2 and D3

Cited by 55 publications

References 21 publications

The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

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