The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

Cortisol and cortisone ratio in urine: LC-MS/MS method validation and preliminary clinical application

“…LC-MS/MS typically provides high analytical sensitivity and specificity, has the ability to simultaneously determine multiple analytes, has a wide dynamic range and usually requires fewer sample preparation steps than gas chromatography-mass spectrometry (GC-MS)-based methods. Because of these advantages, LC-MS/MS is widely accepted in clinical laboratories [17,18].…”

Targeted mass spectrometry: An emerging powerful approach to unblock the bottleneck in phosphoproteomics

“…The SRM approach on triple quadrupole mass spectrometers was first described by Yost and Enke [64] and in combination with the stable isotope dilution (SID) methodology it has become the gold standard quantitative technique in the analysis of small molecules. It is increasingly used as a diagnostic tool in clinical workflows [65] and therefore SRM-based methods have undergone a very rapid expansion in the last decade, for instance, in the screening of inherited metabolic disorders in newborns [66].…”

The clinical utility of mass spectrometry based protein assays

“…Multiple research groups have published concerns regarding the ability of commercial immunoassay kits to distinguish and selectively quantify some proteins including troponin [9], proglucagon-derived peptides [10][11][12], PSA [13] and PTH [14]. Not coincidentally, researchers increasingly explore the use of MS as an alternative to immunoassays [15][16][17][18]. The selectivity of the mass spectrometer has been employed to quantitate heterogeneous large molecules which might exist in multiple isoforms or contain a variable number of post-translational modifications [19].…”