Increased transforming growth factor β and interleukin 10 transcripts in peripheral blood mononuclear cells of colorectal cancer patients

Abstract

Aim of the studyThe ability of immune cells in peripheral blood to produce certain cytokines affects tumour-elicited inflammation. The aim of this study was to investigate the gene expression of interleukin 12A (IL-12A), IL-12B, IL-23A, IL-10, IL-6, transforming growth factor β (TGF-β), HDAC3, and iNOS in peripheral blood mononuclear cells (PBMC) from colorectal cancer (CRC) patients.Material and methodsThe venous blood for PBMC isolation was collected preoperatively and 10 days after surgery, from CRC patients. After isolation of total RNA and synthesis of cDNA, quantitative real-time PCR assays were performed.ResultsOur results demonstrated that among investigated cytokine genes IL-10 and TGF-β were significantly upregulated in patients with CRC compared to the control group, while the expression of IL-23 mRNA was significantly decreased in CRC patients. We observed significantly increased mRNA levels in CRC patients’ PBMC before surgery for IL-10 and TGF-β compared to both postoperative and control groups. We also found a significant upregulation of iNOS in early compared to advanced CRC.ConclusionsBased on the results we can assume that PBMC gene expression programming in CRC patients drives local differentiation of Th cells towards Treg instead of the Th1 anti-tumour subpopulation.

Strategy to targeting the immune resistance and novel therapy in colorectal cancer

“…The commercial HEK‐BlueTM‐hTLR3 cell is expressed through TLR3, which is the subject of host pattern recognition and is responsible for the detection of the virus. In addition, the investigation also showed that the effective expression of the host TLR3 was established by establishing a robust innate immune response with the KRAS‐mutant HCT116 cell line , which inhibited the potential of the virus infection. By lowering the expression of TLR3 and siRNA, the anticancer activity of the virus was improved.…”

Inflammation and Colorectal Cancer

“…The role of IL-10 in CRC remains controversial, as elevated serum levels correlate with poorer survival in humans [25], while several mouse models have demonstrated protective roles of IL-10 in the context of both down regulating inflammation as well as promoting cytotoxic functions. Global IL-10-deficient mice developed severe colitis and adenocarcinoma of the colon by 6 months of age [26].…”

Effects of IL-10 on OX62, MHC-II and CD86 in bone marrow DCs in rats with organophosphate poisoning

“…It plays an important role in autoimmune diseases, severe infectious diseases, cancer and transplantation immune and other diseases by antagonizing the inflammatory mediators ( 21 , 22 ). In the process of immune response, IL-10 can interfere with T lymphocyte activation by inhibiting the antigen presentation, reducing the body's inflammatory response ( 23 , 24 ). For patients with severe organophosphate poisoning, early use of IL-10 and other immunomodulators can reduce the immune system damage to their own tissue ( 12 , 25 ).…”