Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada

Angelis, Karel J.; Břı́za, J.; Šatava, J.; Skákal, I.; Velemínský, J.; Vlasák, Josef; Kleibl, K.; Margison, Geoffrey P.

doi:10.1016/0921-8777(92)90089-l

Cited by 13 publications

(3 citation statements)

References 20 publications

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“…O 6 -methylguanine methyltransferases are widespread and the genes are easily identified on the basis of sequence homology; they are found in bacteria, various fungi, and mammals (Samson, 1992;Nakatsu et al, 1993). Surprisingly, no homologs have been identified in any of the plant DNA sequence data, nor has the activity been identified in plant extracts (Frost and Small, 1987;Angelis et al, 1992). Given the presumed importance of this antimutagenic activity (underlined by its metabolic expense) one can only speculate that plants may have found a better way to cope with this class of lesions.…”

Section: Direct Reversal Of Damagementioning

confidence: 99%

Repair of Damaged Bases

Britt

2002

The Arabidopsis Book

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Section: Direct Reversal Of Damagementioning

confidence: 99%

Repair of Damaged Bases

Britt

2002

The Arabidopsis Book

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“…A. tumefaciens (pAL4404)(pKA 1-1). The pKAI-I plasmid with kanamycin resistance gene has been derived by Angelis et al (1992) from the pGA472 plasmid of An et al (1985). 3.…”

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confidence: 99%

Improvement ofArabidopsis thaliana seed transformation efficiency

Pavingerová¹,

Ondŕej²

1995

Biol. plant.

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Agrobacterium tumefaciens induced transgenosis by treaanent of germinatingArabidopsis thaliana seed embryos has been achieved with different Agrobacterium strains including the strain LBA4404, which was ineffective in seed transformation experiments of the other authors. The frequency of transgenosis was increased several times by application of acetosyringone to the growing A. tumefaciens suspension cultures. The DNA demethylating agent 5-azacytidine partly restored the distorted Mendelian segregation ratios in the offspring of transgenic plants.

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“…Our previous papers (B~ 'iza et al 1989, Babfirek et al 1991, Angelis et al 1992 ) reported results on the toxicity and mutagenicity of alkylating agents in transgenic clones of Nicotiana tabacum var. xanthi n.c. obtained by an Agrobacterium tumefaciens mediated transformation with plasmid pAD2034 harboring the ada gene under the control of dual 1'-2' promoter from Tr-DNA (Velten et al 1984).…”

mentioning

confidence: 99%

Resistance to ACNU induced toxicity in transgenic tobacco suspension cultures withada gene transferred fromEscherichia coli

Břı́za¹,

Angelis²,

Šatava³

et al. 1993

Biol. plant.

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The protein coding region of the E. coli DNA repair gene ada combined with the CaMV 35S promoter has been transferred to tobacco by means of Agrobacterium tumefaciens Ti plasmid. In transgenic plants having the ada gene in a sense orientation, detectable amounts of Ot-alkylguanine-DNA-alkyltransferase has been found whereas in non-transformed plants this activity is absent. Cell suspension cultures derived from the former plants showed lower sensitivity to the toxic (growth inhibiting) effects of the bifunctional alkylating agent 1-(2-chloroethyl)-l-nitroso-3-(aminomethyl-1,3-diazinylo)-methylurea compared with cell cultures derived from a control non-transformed plant or from transgenic plants harbouring the ada gene in an opposite, non-sense orientation.The E. coli DNA repair gene ada encodes enzyme Ot-alkyl-guanine -and alkylphospholriester-DNA-alkyltransferase (ATase) which removes simple alkyl groups from Ot-alkylguanine, O4-alkyl-thymine and from an SP stereoisomer of alkylphospho-triesters (Lindahl et al. 1988). The transfer of this gene into the genome of tobacco enables us to explain the role of resulting DNA lesions and their

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Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada

Cited by 13 publications

References 20 publications

Repair of Damaged Bases

Repair of Damaged Bases

Improvement ofArabidopsis thaliana seed transformation efficiency

Resistance to ACNU induced toxicity in transgenic tobacco suspension cultures withada gene transferred fromEscherichia coli

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