Increased Resistance to Carbapenems inProteus mirabilisMediated by Amplification of theblaVIM-1-Carrying and IS26-Associated Class 1 Integron

Bontron, Séverine; Poirel, Laurent; Kieffer, Nicolas; Savov, Encho; Trifonova, Angelina; Todorova, Irina; Kueffer, Gwendoline; Nordmann, Patrice

doi:10.1089/mdr.2018.0365

Cited by 19 publications

(11 citation statements)

References 22 publications

(25 reference statements)

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“…Not detected were aacC3, bla PSE , bla VIM , bla KPC , and bla IMP . In addition, in the recent 25 yr of reports on the drug resistance of P. mirabilis , the most resistant has been the β-lactamase-producing P. mirabilis ( Bontron et al, 2019 ), which is distributed all over the world and has many types, including broad-spectrum β-lactamase ( ESBLs ) ( Sohn et al, 2011 ), cephalosporinase ( AmpC ) ( Jacoby, 2009 ), and carbapenemase, among which ESBLs are mainly the TEM and CTX-M types ( Nakano et al, 2012 ). In this study, the detection rate of β-lactamase resistance genes was as high as 88% (44/50).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Peng

Zhang

et al. 2022

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Peng

Zhang

et al. 2022

Poultry Science

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“…These strains did not show a gene substrate for OXA-48 synthesis. However, studies have shown that P. mirabilis-CR strains are versatile in terms of carbapenemase production, MBL-NDM, VIMand IMP, 8,[34][35][36] or KPC-producing strains being frequently reported. 6,7,9 With regards to the P. rettgeri strain, this was confirmed to be MBL-NDM type.…”

Section: Discussionmentioning

confidence: 99%

Distribution of NDM1 Carbapenemase-Producing Proteeae Strains on High-Risk Hospital Wards

Rus¹,

Licker²,

Musuroi³

et al. 2020

IDR

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Background: Carbapenem-resistant Proteeae (CRP) is a group of multidrug-resistant (MDR) microorganisms that raise special treatment problems due to their intrinsic resistance to colistin. In this study, our aim is to provide a phenotypic and molecular characterization of the carbapenemases secreted by CRP strains isolated from inpatients from an intensive care unit (ICU) and surgical wards, as well as the identification of the risk factors involved in their acquisition. Methods: An observational, cross-sectional study was performed which included all Proteeae strains isolated in samples from inpatients on high-risk wards of the largest university hospital in Western Romania, from July 2017 to April 2019. Meropenemresistant strains (N=65) with MIC ≥16 µg/mL were subjected to a singleplex PCR assay for the detection of blaNDM, blaVIM and blaCTX-M genes. The analysis of risk factors was performed by logistic regression. Results: Out of 8317 samples that were processed, 400 Proteeae strains were isolated: 64% belonging to the genus Proteus, 26.75% to the genus Providencia and 9.25% to the genus Morganella. Most CRP strains (N=56) were of MBL type, and 55 had the blaNDM gene as the prevalent gene substrate. P. stuartii was the main species that provided the circulating MDR strains. Most CRP strains came from patients admitted to ICU, being isolated mainly from bronchial aspirates and blood cultures. Multivariate analysis revealed 3 independent risk factors -mechanical ventilation>96h ], p <0.001), tracheostomy (HR: 2.65 [1.14-6.17], p = 0.024) and prolonged antibiotic therapy (HR: 1.01 [1.00--1.02], p = 0.03). Conclusion:There is a significant increase in the incidence of CR P. stuartii strains, the MBL-blaNDM type being predominant. These strains presented various other resistance mechanisms, being often extremely difficult to treat and led to an excess of lethality of 27.16%.

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“…These genes may be carried on genetic elements such as plasmids, transposons and on integrons that can be mobilized [12]. Previously, the emergence of multidrug-resistant P. mirabilis-producing extended spectrum beta lactamase, AmpC, and carbapenemases has been reported [19,20]. Since there is a trend of developing resistance to various antimicrobial drugs, it is important to investigate new drugs, combination therapies or methods to overcome antimicrobial resistance problems caused by P. mirabilis bacteria.…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of hyperthermia on swarming motility and antimicrobial susceptibility profiles of Proteus mirabilis isolates

Gazel

Demirbakan

Erinmez

2021

International Journal of Hyperthermia

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Aim: Swarming motility is a virulence factor for Proteus mirabilis and is a coordinated multicellular movement of bacteria. In this study, we investigated the inhibitory effect of hyperthermia on bacterial swarming motility and antimicrobial resistance. Methods: Thirty-one P. mirabilis isolates were included in the study. Seven inoculated agar plates were incubated inside incubators with increasing temperature levels: at 36 C (control) and 40-45 C. On the next day, inhibition of swarming was evaluated and minimum paralyzing temperature (MPT) values were determined. An antimicrobial susceptibility test (antibiogram) is performed by exposing bacteria to increasing concentrations of antibiotics, in vitro. Thus, we used the Kirby-Bauer disk diffusion test as a screening method to analyze the antibiogram profiles of the isolates at 36 C and 42 C. Finally, a time-kill assay was performed to analyze the killing effect of hyperthermia (42 C) on planktonic bacteria, in combination with the antibiotic meropenem at the first and third hours. A Wilcoxon signed-rank test was used to compare the killing effects of meropenem, hyperthermia and their combinations. Results: The median MPT value was determined as 44 C. In the disk diffusion assay, susceptibility development was observed in 94% of isolates for at least one antibiotic. In the time-kill assay, we observed a significant killing effect of hyperthermia in combination with meropenem. Under the microscope, we observed the formation of spherical cells by the effect of heat. Conclusion:We conclude that these findings might be useful when employing the hyperthermia method to treat infectious diseases caused by P. mirabilis in the future.

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Increased Resistance to Carbapenems inProteus mirabilisMediated by Amplification of thebla_VIM-1-Carrying and IS26-Associated Class 1 Integron

Cited by 19 publications

References 22 publications

Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

Distribution of NDM1 Carbapenemase-Producing Proteeae Strains on High-Risk Hospital Wards

In vitro activity of hyperthermia on swarming motility and antimicrobial susceptibility profiles of Proteus mirabilis isolates

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