Increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in England and Wales since 2001

Strong, Rebecca; Errington, Jane; Cook, Richard T.; Ross-Smith, Natalie; Wakeley, Philip R.; Steinbach, Falko

doi:10.1016/j.vetmic.2012.09.006

Cited by 26 publications

(32 citation statements)

References 22 publications

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“…Results of a larger study of 316 isolates collected in England and Wales by Strong and others (Strong et al 2013) between 2004 and 2009 were consistent with the finding of Booth et al (2013) that BVDV-1a was the dominant subgenotype.…”

Section: Prevalence Of Bvdv Genotypes and Sub-genotypessupporting

confidence: 78%

“…However, in contrast, Strong et al (2013) identified one isolate to be BVDV-1f and no BVDV-1d viruses were detected in the sample population analysed. Both of these recent studies are in agreement that there has been the introduction of new BVDV subgenotypes, BVDV-1d, 1e and 1f, into the UK since an earlier study of 62 field samples collected during 1996 and 1997 (Vilcek et al 1999b).…”

Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 61%

“…However, a recent study identified that amino acid changes occurred in BVD viruses which shared 93% to 99% homology within their N pro (Strong et al 2013). The present study will act as a reference or baseline from which future phylogenetic studies can be interpreted.…”

Section: Resultsmentioning

confidence: 82%

“…The Neighbour-Joining method was found to be an appropriate method when analysing a large number of short nucleotide sequences with a low level of divergence. This was confirmed by the fact that number of similar phylogenetic studies analysing BVDV sequences also exploited this method (Booth et al 2013;Strong et al 2013). …”

Section: Information (Ncbi) Genbank Databasementioning

confidence: 75%

“…Numerous studies have been conducted internationally to identify the genotypes and sun-genotypes of BVDV in circulation within individual countries or regions (Booth et al 2013;Strong et al 2013;Vilcek et al 1999;Graham et al 2001;Arias et al 2003). Little is known about the genotypes of BVDV circulating in Irish cattle herds.…”

mentioning

confidence: 99%

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Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

O’Brien

Garvey

Walsh

et al. 2017

Research in Veterinary Science

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The genotype and subgenotype was established for 325 field viruses and one commercial vaccine viral strain based on the analysis of at least one genomic region. BVDV-1a was the prominent subgenotype (n=317) with BVDV-1b (n=6), BVDV-1d (n=1) and BVDV-1e (n=1) also observed. A number of nucleotide sequence alignments and phylogenetic trees were constructed to represent the relationship between Irish field viruses and reference strains representative of all known BVDV genotypes and subgenotypes. Evidence of both herd specific clustering and circulation of multiple strains within herds was observed; however no evidence of county specific clustering was observed. A number of completely conserved nucleotide regions were observed and the 5'UTR was found to be more conserved than the N pro fragment analysed. The results of this study provide a detailed and comprehensive insight into the genetic diversity of BVDV in circulation within Irish cattle herds and will act as a baseline reference for future BVDV genotyping studies.

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Section: Prevalence Of Bvdv Genotypes and Sub-genotypessupporting

confidence: 78%

Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 82%

Section: Information (Ncbi) Genbank Databasementioning

confidence: 75%

mentioning

confidence: 99%

See 3 more Smart Citations

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

O’Brien

Garvey

Walsh

et al. 2017

Research in Veterinary Science

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Molecular diversity of bovine viral diarrhea virus in uruguay

Maya

Puentes

Reolón³

et al. 2015

Arch Virol

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Bovine viral diarrhea (BVD) affects bovine production and reproduction causing significant economic losses all over the world. Two viral species has been recognized: BVDV-1 and BVDV-2, both distributed worldwide. Recently, novel specie of BVDV named HoBi-like pestivirus was discovered. The presence of BVDV was confirmed in 1996 in Uruguay, however, does not exist until today a schedule of compulsory vaccination along the country. Serological studies with samples from all Uruguayan herds were performed during 2000 and 2001 demonstrating that all of them were seropositive to BVDV with a mean prevalence of 69%. In addition, there have been no new studies done since those previously described and it is important to mention that the genetic diversity of BVD has never been described in Uruguay. Nowadays, there is strongly suspect that BVDV is one of the most important causes of reproductive failures in our herds. The aim of this study was to describe for the first time in Uruguay the genetic diversity of BVDV with samples collected from different regions along the country. Serological status of 390 non-vaccinated animals against BVDV with reproductive problems from farms of Rivera, Tacuarembó and Florida departments of Uruguay were studied. All herds were seropositive to BVDV and high proportion of animals were positive (298/390), while 4.1% (16/390) of the animals were positive to Antigen Capture ELISA test and Real Time PCR. Phylogenetic analysis performed with concatenated sequences from the 5'UTR and Npro genomic regions revealed that BVDV-1 and BVDV-2 are infecting our herds, being BVDV-1 the most frequently found. The major subtype was BVDV-1a, followed by BVDV-1i and BVDV-2b. This is the first study that describes the genetic diversity of BVDV in Uruguay and it will contribute to the elaboration of sanitization programs.

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Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil

et al. 2016

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Bovine viral diarrhea virus 1 (BVDV-1) belongs to the genus Pestivirus within the family Flaviviridae. Based on the 5' untranslated region (UTR) sequence, BVDV-1 can be divided into at least 17 subtypes (1a though 1q). BVDV-1i is an uncommon subtype that has been reported in the United Kingdom and Uruguay. Here, we report the complete genome sequence of the first subtype 1i BVDV-1 (strain ACM/BR/2016) isolated from cattle in southern Brazil. The genome is 12,231 nt in length and contains a single ORF that encodes a polyprotein of 3,896 amino acids, flanked by 5' and 3'UTRs of 325 and 220 nt, respectively. Phylogenetic inferences based on the whole genome, the 5'UTR, and the N region showed that strain ACM/BR/2016 is closely related to previously characterized BVDV-1i members. Its 5'UTR shares the highest nucleotide identity (90.5%) with BVDV-1i strains from United Kingdom, and its N is most closely related to that of a Uruguayan strain (90.6%). To the best of our knowledge, this is the first BVDV-1i strain from which the whole genome has been completely sequenced and characterized. The complete genome of a BVDV-1i will help future studies on pestivirus evolution and heterogeneity.

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Increased phylogenetic diversity of bovine viral diarrhoea virus type 1 isolates in England and Wales since 2001

Cited by 26 publications

References 22 publications

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

Molecular diversity of bovine viral diarrhea virus in uruguay

Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil

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