Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil

Mósena, Ana Cristina Sbaraini; Weber, Matheus Nunes; Cibulski, Samuel Paulo; Silveira, Simone; Silva, Mariana S.; Mayer, Fabiana Quoos; Canal, Cláudio Wageck

doi:10.1007/s00705-016-3199-7

Cited by 14 publications

(24 citation statements)

References 17 publications

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“…BVDV-1a (cases 1, 5, and 6) and BVDV-2b (cases 3 and 4) were identified in heifers/steers in our work, indicating that both species are related to postnatal morbidity and mortality in cattle in Uruguay. In other South American countries, clinical disease has been reported for BVDV-1a, BVDV-1b, BVDV-2a, and BVDV-2b in Argentina [44][45][46]; BVDV-1b, BVDV-1d, BVDV-1i, and BVDV-2b in Brazil [47][48][49][50]; and BVDV-1a, BVDV-1b, and BVDV-1c in Chile and Peru [17,51]. Although cattle exposed to BVDV in utero constitute a significant portion of clinical presentations, cases of transient/acute postnatal infection may be significant sources of direct losses [1].…”

Section: Discussionmentioning

confidence: 99%

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

et al. 2019

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Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector.

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Section: Discussionmentioning

confidence: 99%

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

et al. 2019

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“…: Genotyping was not performed. References (I): [48,49,50,51,52]; (II): [7,43,49,52,53,54,55,56,57,58]; (III): [59,60]; (IV): [39,44,49,61,62,63,64,65,66,67,68,69,70]; (V): [49,71]; (VI): [72]. In [57] the year of virus isolation was not displayed in the study.…”

Section: Figurementioning

confidence: 99%

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Yeşilbağ

Alpay

Becher

2017

Viruses

189

221

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Bovine viral diarrhea virus (BVDV) is a globally-distributed agent responsible for numerous clinical syndromes that lead to major economic losses. Two species, BVDV-1 and BVDV-2, discriminated on the basis of genetic and antigenic differences, are classified in the genus Pestivirus within the Flaviviridae family and distributed on all of the continents. BVDV-1 can be segregated into at least twenty-one subgenotypes (1a–1u), while four subgenotypes have been described for BVDV-2 (2a–2d). With respect to published sequences, the number of virus isolates described for BVDV-1 (88.2%) is considerably higher than for BVDV-2 (11.8%). The most frequently-reported BVDV-1 subgenotype are 1b, followed by 1a and 1c. The highest number of various BVDV subgenotypes has been documented in European countries, indicating greater genetic diversity of the virus on this continent. Current segregation of BVDV field isolates and the designation of subgenotypes are not harmonized. While the species BVDV-1 and BVDV-2 can be clearly differentiated independently from the portion of the genome being compared, analysis of different genomic regions can result in inconsistent assignment of some BVDV isolates to defined subgenotypes. To avoid non-conformities the authors recommend the development of a harmonized system for subdivision of BVDV isolates into defined subgenotypes.

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“…In addition to D32/00_'HoBi' (Schirrmeier et al, 2004), at least four full genomic sequences of pestiviruses isolated in Brazil have been published: three HoBiPeV and one BVDV-1i. Mósena et al (2017aMósena et al ( , 2017b performed a full genomic sequencing and characterization of a BVDV-1i isolated from cattle in RS state. BVDV-1i is an uncommon subgenotype and has been detected in few regions (Yeşilbağ et al, 2017).…”

Section: Complete Genome Sequencingmentioning

confidence: 99%

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

Flores

Cargnelutti

Monteiro

et al. 2018

Anim. Health. Res. Rev.

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The pestiviruses bovine viral diarrhea virus 1 (BVDV-1), 2 (BVDV-2), and HoBi-like (HoBiPeV) are endemic among Brazilian cattle, the world's largest commercial bovine herd. In the last two decades (1998–2018) over 300 bovine pestiviruses have been partially or fully sequenced in Brazil, including viruses from different regions, different epidemiological backgrounds, and associated with diverse clinical presentations. Phylogenetic analysis of these viruses demonstrated a predominance of BVDV-1 (54.4%), with subgenotypes −1a (33.9% of total) and −1b (16.3%) being more frequent and subgenotypes −1d, −1e, and −1i at very low frequencies. The overall BVDV-2 frequency was 25.7% but it varied largely by region, reaching up to 48% in Southern states. BVDV-2b was the predominant subgenotype (84.8% of BVDV-2), followed by BVDV-2a (8.86%). HoBiPeV accounted for 19.9% (61/307) of the genotyped viruses and were detected at high frequency in cattle from Northeastern states. These findings demonstrate a unique mix of pestivirus species and subgenotypes, unlike that seen in Europe or North America. The design of effective diagnostic tools, vaccines, and control programs for limiting bovine pestivirus infections in Brazil must take into consideration this unique mix of viruses. This article provides a critical review of two decades of genetic identification of pestiviruses in Brazil.

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Genomic characterization of a bovine viral diarrhea virus subtype 1i in Brazil

Cited by 14 publications

References 17 publications

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

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