Increased extracellular levels of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 in minor salivary glands of patients with Sjögren's syndrome

Ek, Monica; Popovic, Karin; Harris, Helena Erlandsson; Nauclér, Cecilia Söderberg; Wahren‐Herlenius, Marie

doi:10.1002/art.21969

Cited by 69 publications

(71 citation statements)

References 15 publications

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“…Kinetics of HMGB1 Oxidation and Clearance in Saliva-Salivary gland cells are known to produce extracellular HMGB1 (38). To examine whether or not the molecular behavior of Intensities were measured for NMR signals from residues that exhibit relatively strong, well isolated signals for both all-thiol and disulfide states (i.e.…”

Section: Nmr Of All-thiol and Disulfide Hmgb1 Proteins Dissolved Inmentioning

confidence: 99%

Real-time Kinetics of High-mobility Group Box 1 (HMGB1) Oxidation in Extracellular Fluids Studied by in Situ Protein NMR Spectroscopy

Zandarashvili

Sahu

Lee

et al. 2013

Journal of Biological Chemistry

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Background: Redox of extracellular HMGB1 protein plays an important role in inflammation. Results: The half-life of all-thiol HMGB1 was ϳ17 min in serum and saliva and significantly longer in cancer cell culture medium and was modulated by exogenous ligands (e.g. heparin). Conclusion:The extracellular environment dictates HMGB1 oxidation kinetics. Significance: Our approach permits investigating protein oxidation in situ.

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Section: Nmr Of All-thiol and Disulfide Hmgb1 Proteins Dissolved Inmentioning

confidence: 99%

Real-time Kinetics of High-mobility Group Box 1 (HMGB1) Oxidation in Extracellular Fluids Studied by in Situ Protein NMR Spectroscopy

Zandarashvili

Sahu

Lee

et al. 2013

Journal of Biological Chemistry

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“…In conclusion, HMGB-1 and sRAGE may be a key molecule and serological marker that links 3 main SSc features of immunological abnormalities, fibrosis, and vascular damage. 41 TSS, %VC, %DLco, the pulsatility index (PI) value, and serum levels of IgG in patients with SSc. The PI value is a parameter for renal vascular resistance determined by color-flow Doppler ultrasonography of the renal interlobar arteries of both kidneys.…”

Section: Serum Levels Of Hmgb-1 and Srage In Sscmentioning

confidence: 99%

“…Moreover, co-expression of HMGB-1, TNF-α, IL-1β, and IL-6 has been reported in several autoimmune diseases, such as systemic lupus erythematosus and Sjögren's syndrome [41,42]. However, the association of serum levels of HMGB-1 and those of several cytokines, including TNF-α, IL-1β, and IL-6 has been controversial.…”

mentioning

confidence: 99%

Clinical Significance of Serum HMGB-1 and sRAGE Levels in Systemic Sclerosis: Association with Disease Severity

et al. 2008

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The high mobility group box 1 protein (HMGB-1)/adcanced glycation end products (RAGE) system is recently shown to play an important part in immune/inflammatory disorders. However, the association of this system in systemic sclerosis (SSc) remains unknown. To determine clinical association of serum levels of HMGB-1 and soluble RAGE (sRAGE) in patients with SSc, sera from 70 patients with SSc and 25 healthy controls were examined by enzyme-linked immunosorbent assay. Sera from Tight-skin mice and bleomycin-induced scleroderma mice, animal models for SSc, were also examined. Skin HMGB-1 and RAGE expression was assessed by immunohistochemistry. Serum HMGB-1 and sRAGE levels in SSc were higher than those in controls. Similarly, HMGB-1 and sRAGE levels in animal SSc models were higher than those in control mice. SSc patients with elevated HMGB-1 and sRAGE levels had more frequent involvement of several organs and immunological abnormalities compared to those with normal levels. Furthermore, HMGB-1 and sRAGE levels correlated positively with modified Rodnan total skin thickness score and negatively with pulmonary function test. HMGB-1 and sRAGE expression in the sclerotic skin was more intense than normal skin. These results suggest that elevated serum HMGB-1 and sRAGE levels are associated with the disease severity and

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“…Consequently, the released HMGB1 activates the monocytes to stimulate further release of proinflammation cytokines such as TNF-, IL-1 and IL-6, forming a positive feedback loop [4,5].…”

Section: Introductionmentioning

confidence: 99%

Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis patients and peri-implantitis

Luo

Xie

Gong

et al. 2011

Archives of Oral Biology

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High mobility group chromosomal protein B1 (HMGB1) and N2 (HMGN2), two members of High mobility group (HMG) family, play important role in inflammation. The purposes of this study were to investigate the expression of HMGB1 and HMGN2 in periodontistis.The expression of HMGB1 and HMGN2 mRNA in gingival tissues and gingival crevicular fluid (GCF) in chronic periodontitis (CP), generalized aggressive periodontitis (G-AgP) patients and healthy subjects was detected by real-time PCR. The protein level of HMGB1 and HMGN2 in peri-implant crevicular fluid (PICF), peri-implant crevicular fluid of peri-implantitis (PI-PICF) and normal patients was determined by Western blotting. Furthermore, IL-1 , IL-6, IL-8, TNF-and HMGB1 levels in GCF, PI-PICF and healthy-PICF samples from different groups were determined by ELISA.HMGN2 expression was increased in inflamed gingival tissues and GCF from CP and G-ApG groups compared to control group. HMGB1 expression was the highest in the gingival tissues and GCF from CP patients and was accompanied by increased concentrations of IL-1 , IL-6, IL-8 proinflammaory cytokines.To our knowledge, this is the first study reporting that the expression of HMGB1 and HMGN2 was increased in the gingival tissues and GCF in CP and G-AgP and the PICF in PICF. Our data suggest that HMGB1 may be a potential target for the therapy of periodontitis and PI.

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Increased extracellular levels of the novel proinflammatory cytokine high mobility group box chromosomal protein 1 in minor salivary glands of patients with Sjögren's syndrome

Cited by 69 publications

References 15 publications

Real-time Kinetics of High-mobility Group Box 1 (HMGB1) Oxidation in Extracellular Fluids Studied by in Situ Protein NMR Spectroscopy

Real-time Kinetics of High-mobility Group Box 1 (HMGB1) Oxidation in Extracellular Fluids Studied by in Situ Protein NMR Spectroscopy

Clinical Significance of Serum HMGB-1 and sRAGE Levels in Systemic Sclerosis: Association with Disease Severity

Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis patients and peri-implantitis

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