Increased expression of MMP-2 and MMP-9 indicates poor prognosis in glioma recurrence

Zhou, Wei; Yu, Xianpeng; Sun, Shuang; Zhang, Xuehai; Yang, Weixiao; Zhang, Junpeng; Zhang, Xin; Jiang, Zheng

doi:10.1016/j.biopha.2019.109369

Cited by 87 publications

(78 citation statements)

References 32 publications

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“…34,35 Both MMP-2 and MMP-9 are critical members of the matrix metalloproteinase (MMP) family, and overexpression of these proteins significantly promotes tumor invasion and migration. 36,37 This study revealed that the role of MT1JP overexpression in inhibiting the invasion and migration of breast cancer cells may be attributed primarily to the inhibition of MMP-2 and MMP-9 expression.…”

Section: Discussionmentioning

confidence: 89%

<p>lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis</p>

Ouyang

Cui

Yang

et al. 2020

OTT

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Introduction: The purpose of our research was to evaluate MT1JP in breast cancer. Material and Methods: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism's proteins were evaluated by Western blotting or immunohistochemistry assay. Results: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P<0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/ RUNX3 axis. Conclusion: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study.

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Section: Discussionmentioning

confidence: 89%

<p>lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis</p>

Ouyang

Cui

Yang

et al. 2020

OTT

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“…It is also a key component of many important enzymes, such as matrix metalloproteinase (MMP) and carbonic anhydrase [24], which are related to tumor proliferation and metastasis [25][26][27][28]. Studies have shown that MMP2\MMP9 are zinc-dependent proteolytic enzymes involved in the remodeling and degradation of extracellular matrix (ECM) and can enhance the invasion and metastasis of glioma [29][30][31]. In this study, GO analysis has shown that SLC39A1 is mainly enriched in extracellular matrix organization, and extracellular structure organization, etc.…”

Section: Discussionmentioning

confidence: 99%

SLC39A1 contribute to malignant progression and have clinical prognostic impact in gliomas

Wang

Zhang

et al. 2020

Cancer Cell Int

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Background Gliomas are one of the most common primary tumors of the central nervous system, and have an unfavorable prognosis. SLC39A1 is a zinc ion transport protein which inhibits the progression of prostate cancer. By studying the role and mechanism of SLC39A1 in the progression of gliomas, perhaps a new therapeutic target can be provided for their treatment. Method The TCGA, CCGA, GSE16011, GSE44971 and GSE11260 data sets were employed to evaluate the expression level of SLC39A1 in paracancerous and glioma tissues. In addition, Kaplan–Meier analysis, Cox analysis, and the ESTIMATE and CIBERSORT algorithms were used to analyze its prognostic value and immune infiltration correlation. A CCK-8 and flow cytometer were used to measure the effects of SLC39A1 on U87 cell proliferation or apoptosis; RT-qPCR and western blot were used to detect its effects on the expression of MMP2\MMP9. Results SLC39A1 has up-regulated expression in glioma tissues. High SLC39A1 expression predicted significantly worse survival. Univariate and multivariate analysis show that SLC39A1 independently indicated poor prognosis in patients with gliomas. The expression of SLC39A1 is significantly correlated with clinical pathological parameters such as Grade, IDH mutation status, and 1p19q codeletion status. In vitro experimental results show that SLC39A1 promotes proliferation of glioma cells, inhibits their apoptosis, and promotes expression of MMP2\MMP9. In addition, it may affect infiltration of immune cells into the glioma microenvironment. Conclusion SLC39A1 may serve as a new prognostic biomarker and potential target for treatment of gliomas.

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“…The disruption of the extracellular matrix (ECM) has been identified as an initial step in the penetration of glioma cells into adjacent normal brain tissue ( 38 ). Emerging evidence indicates that MMP-2, which is an important member of the MMP family, is required for the degradation of the physical ECM barrier at tumour invasion fronts during this process ( 39 , 40 ). Notably, the expression level of MMP-2 increases as the pathological stage advances and is the highest in malignant gliomas ( 39 ).…”

Section: Discussionmentioning

confidence: 99%

“…Emerging evidence indicates that MMP-2, which is an important member of the MMP family, is required for the degradation of the physical ECM barrier at tumour invasion fronts during this process ( 39 , 40 ). Notably, the expression level of MMP-2 increases as the pathological stage advances and is the highest in malignant gliomas ( 39 ). In particular, MMP-2 is a well-documented candidate predictive biomarker of glioma invasion ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑138 modulates glioma cell growth, apoptosis and invasion through the suppression of the AKT/mTOR signalling pathway by targeting CREB1

et al. 2020

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Alterations in the expression of microRNA (miR)-138 have been demonstrated to result in the development of several malignant tumours. However, the possible function of miR-138 in human glioma cells remains unclear. The present study demonstrated that miR-138 was significantly downregulated in 48 human glioma specimens by quantitative PCR analysis. The upregulation of miR-138 exerted significant antiproliferative and anti-invasive effects on glioma cells and promoted their apoptosis. In addition, cAMP response element-binding protein 1 (CREB1) was confirmed as a direct target gene of miR-138 by luciferase gene reporter assay, and the antitumour effect of miR-138 on glioma cells was significantly reversed by CREB1 overexpression. Moreover, the molecular mechanisms underlying the tumour-suppressive role of miR-138 in malignant glioma may be associated with the dephosphorylation of AKT/mTOR caused by the miR-138 upregulation-induced decrease in CREB1 expression in glioma cells. The results of the present study indicated that miR-138 may affect CREB1/AKT/mTOR signalling to regulate the proliferation, apoptosis and invasion of glioma cells and the malignant progression of glioma, thereby suggesting that miR-138 may be a potential target for the treatment of gliomas.

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Increased expression of MMP-2 and MMP-9 indicates poor prognosis in glioma recurrence

Cited by 87 publications

References 32 publications

<p>lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis</p>

<p>lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis</p>

SLC39A1 contribute to malignant progression and have clinical prognostic impact in gliomas

MicroRNA‑138 modulates glioma cell growth, apoptosis and invasion through the suppression of the AKT/mTOR signalling pathway by targeting CREB1

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