Increase of transforming growth factor-β1 in gingival crevicular fluid during human orthodontic tooth movement

Uematsu, Setsuko; Mogi, Makio; Deguchi, Toshio

doi:10.1016/s0003-9969(96)00063-5

Cited by 62 publications

(42 citation statements)

References 19 publications

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“…Among these studies, further analysis of the titles, abstracts, and fulltexts revealed that 25 were relevant for the present study. A summary of papers that were excluded is presented in Figure 1 (exhibiting the orthodontic mechanics, GCF sampling methods, and cytokine measurements) [1][2][3][4][5][6][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41].…”

Section: Resultsmentioning

confidence: 99%

Cytokines in crevicular fluid and orthodontic force: a systematic review

Allgayer

Menezes

Rinaldi

2017

Rev. Odonto Ciênc. (Online)

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OBJECTIVE: This paper aimed to analyze studies in the literature addressing the role of cytokines in the gingival crevicular fluid (GCF) in the orthodontic treatment. METHODS: Several databases were surveyed using both MESH terms and free terms. Additional studies were obtained by reference tracking. This review was registered in PROSPERO, and the procedures foreseen by its statement were followed. Data were obtained from the included studies addressing the orthodontic mechanics, GCF sampling/handling methods, and cytokine measurements. Clinical studies written in English were browsed. Papers were selected by one reviewer and checked by a second investigator. RESULTS: A total of 115 articles were identified, among which 25 were selected for detailed analysis. Common drawbacks consisted mainly of inadequacies in the study design (e.g. short duration and small number of study subjects). The most consistent result was a peak of cytokine levels at 1 d. Associations were observed between prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) and pain, velocity of tooth movement, and treatment mechanics. Interleukin-1b and PGE2 showed different patterns of up-regulation, with IL-1β being more responsive to mechanical stress and PGE2 more responsive to synergistic regulation of IL-1β and mechanical force. The results support the use of light continuous forces for orthodontic treatment. CONCLUSION: There was a tendency of maintenance of relatively high IL-1β levels for longer periods with the use of light continuous forces, which might decrease the frequency of activation. These outcomes provide evidence at the cellular level for the utilization of light continuous forces.Key words: Systematic review; Orthodontics, corrective; Gingival Crevicular Fluid (GCF); Interleukin; T Cells.Papel das citocinas presentes no fluido crevicular durante o tratamento ortodôntico: uma revisão sistemática REsUMO OBJETIVO: Este artigo teve como objetivo analisar estudos na literatura abordando o papel das citocinas no Líquido do Sulco Gengival no tratamento ortodôntico. MéTODOS: Várias bases de dados foram pesquisadas usando termos MESH e termos livres. Estudos adicionais foram obtidos por rastreamento de referência. Esta revisão foi registrada no PROSPERO, e os procedimentos previstos por sua declaração foram seguidos. Os dados foram obtidos dos estudos incluídos sobre a mecânica ortodôntica, métodos de amostragem / manuseio de Líquido do Sulco Gengival e medições de citoquinas. Estudos clínicos escritos em inglês foram pesquisados. Os trabalhos foram selecionados por um revisor e verificados por um segundo investigador. RESULTADOS: Foram identificados 115 artigos, dentre os quais 25 foram selecionados para análise detalhada. As desvantagens comuns consistiram principalmente em insuficiências no design do estudo (por exemplo, curta duração e pequeno número de sujeitos do estudo). O resultado mais consistente foi um pico de níveis de citocinas a 1 d. Foram observadas associações entre prostaglandina E2 (PGE2) e interleucina-1β (IL-1β) ...

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Section: Resultsmentioning

confidence: 99%

Cytokines in crevicular fluid and orthodontic force: a systematic review

Allgayer

Menezes

Rinaldi

2017

Rev. Odonto Ciênc. (Online)

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“…[1][2][3][4][5][6][7][8][9] The analysis of GCF is a useful and promising method to monitor the changes at a single site during a certain method. 4,5,10 We used the same time intervals of 0, 1, 24, and 168 hours after initiation of orthodontic tooth movement as did previous studies of GCF samples.…”

Section: Discussionmentioning

confidence: 99%

“…Mechanical stress from orthodontic appliances is believed to induce cells in the periodontal ligament (PDL) to form biologically active substances, such as enzymes and cytokines, responsible for connective tissue remodeling. [1][2][3][4][5][6][7][8][9] Therefore, biochemical analysis of the gingival crevicular fluid (GCF) has provided a noninvasive model for investigating the cellular response of the underlying PDL during orthodontic tooth movement in vivo. 10 In the GCF, several substances such as interleukin (IL)-1b, IL-6, tumor necrosis factor (TNF)-a, b 2 microglobulin, osteocalcin, and alkaline phosphatase have been found to be significantly elevated in teeth undergoing orthodontic forces compared with untreated controls.…”

Section: Introductionmentioning

confidence: 99%

Leptin Levels in Gingival Crevicular Fluid During Orthodontic Tooth Movement

Dilsiz¹,

Kılıç²,

Aydın³

et al. 2010

The Angle Orthodontist

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Objectives: To test if leptin can be detected in the gingival crevicular fluid (GCF) around moving teeth, and to determine whether any changes occur during orthodontic tooth movement. Materials and Methods: An upper canine requiring distal movement served as the test tooth; the contralateral canine was used as a control tooth. The control tooth was included in the orthodontic appliance, but was not subjected to the orthodontic force. GCF sampling from the distal sites of the test and control teeth was done at baseline, 1 hour, 24 hours, and 168 hours. Results: Leptin concentrations of the test teeth decreased in a time-dependent manner. When compared with the baseline measurement, the decrease was significant at 168 hours (P , .05). Conclusions:The concentration of leptin in GCF is decreased by orthodontic tooth movement; the results of the present study also suggest that leptin may have been one of the mediators responsible for orthodontic tooth movement. (Angle Orthod. 2010;80:504-508.)

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“…6) TGF-β1 was rapidly and transiently increased in orthodontic tooth movement and was associated with bone remodeling in vivo. 9) On the other hand, TGF-β1 blocked osteogenesis by a variety of mechanisms depending on cell density, TGF-β1 concentration and differentiation stage of the cells. [10][11][12] TGF-β1 also blocked odontogenesis by downregulation of dentin sialophosphoprotein.…”

Section: Introductionmentioning

confidence: 99%

Dual Nature of TGF-β1 in Osteoblastic Differentiation of Human Periodontal Ligament Cells

Ochiai

Yamamoto

Yokoyama

et al. 2010

J. Hard Tissue Biology.

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This study investigated the effect of various treatments with TGF-β1 in osteogenic differentiation of human periodontal ligament (HPDL) cells.HPDL cells were treated with single 1 ng/ml, 100 ng/ml or multiple (12-h intervals) 1 ng/ml TGF-β1. Alkaline phosphatase (ALP) activity was detected by ALP activity staining at 4 days. Phospho-Smad3 was detected by western blotting and transcription of osteogenic differentiation markers (RUNX2, ALPL, IBSP: bone sialoprotein, BGLAP: osteocalcin) were determined by real-time PCR at various culture periods. Cells were pretreated with or without SB505124, a TGF-β/Smad pathway inhibitor, for 1 h before 1 ng/ml TGF-β1 treatment at 12-h intervals. ALP activity and phosphorylated Smad3 were detected.Single treatment with 1 ng/ml TGF-β1 significantly induced Smad3 phosphorylation, subsequent ALP upregulation and transcription of osteogenic differentiation markers, compared to these inductions with 100 ng/ml TGF-β1 treatment. In multiple treatments with 1 ng/ml TGF-β1 at 12-h intervals, only the first treatment significantly induced Smad3 phosphorylation but not the succeeding treatments. Inductive effects of single 1 ng/ml TGF-β1 in osteogenic differentiation were almost completely abolished by multiple treatments. Furthermore, multiple treatments with 1 ng/ml TGF-β1 did not induce ALP activity although the TGF-β/Smad pathway was inhibited by SB505124.Single treatment with low dose TGF-β1 significantly induced osteogenic differentiation. Multiple treatments inhibited osteogenic differentiation despite inhibition of TGF-β/Smad pathway. These findings suggest that TGF-β1 both positively and negatively regulates osteogenic differentiation and the inhibitory effect may be mediated by the non-Smad signaling pathway in HPDL cells.

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Increase of transforming growth factor-β1 in gingival crevicular fluid during human orthodontic tooth movement

Cited by 62 publications

References 19 publications

Cytokines in crevicular fluid and orthodontic force: a systematic review

Cytokines in crevicular fluid and orthodontic force: a systematic review

Leptin Levels in Gingival Crevicular Fluid During Orthodontic Tooth Movement

Dual Nature of TGF-β1 in Osteoblastic Differentiation of Human Periodontal Ligament Cells

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