Increase of Hepatic mRNAs of Profilin, Actin and Extracellular Matrix Proteins After Carbon Tetrachloride Treatment and Partial Hepatectomy in Rats

Nakamura, H.; Hirata, Kyoji; Yamashiro, Keisuke; Hiranuma, Kenji; Shibata, Keitaro; Higashi, Ken; Morita, Tsuyoshi; Hirano, Hideyasu

doi:10.1006/bbrc.1994.1083

Cited by 15 publications

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“…The Northern blots were subsequently stripped and reprobed with oligonucleotide probes for ␤-actin or constitutively expressed 28S RNA. The amount of the 2.2-kb ␤-actin mRNA did not change within the first 2 h after partial hepatectomy, whereas at 6, 16, and 24 h, there were marked increases (data not shown), which is in agreement with several published reports (17,45). The unchanged intensity of the 28S RNA band in each lane (not shown) further indicated that the observed decrease of ␣ 1b AR mRNA after partial hepatectomy was real and not the result of uneven loading or transfer of mRNAs to the membranes.…”

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confidence: 92%

Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver

Gao

Liu

Kunos

1996

Molecular and Cellular Biology

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The 5 upstream region from ؊490 to ؊540 (footprint II) within the dominant P2 promoter of the rat ␣ 1b adrenergic receptor (␣ 1b AR) gene is recognized by a sequence-specific DNA-binding protein (B. Gao, M. S. Spector, and G. Kunos, J. Biol. Chem. 270:5614-5619, 1995). This protein, detectable in Southwestern (DNAprotein) blots of crude nuclear extracts as 32-and 34-kDa bands, has been purified 6,000-fold from rat livers by DEAE-Sepharose, heparin-Sepharose, and DNA affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and UV cross-linking of the purified protein indicated the same molecular mass as that in crude extracts. Methylation interference analysis revealed strong contact with a TTGGCT hexamer and weak contact with a TGGCGT hexamer in the 3 and 5 portions of footprint II, respectively. Nucleotide substitutions within these hexamers significantly reduced protein binding to footprint II and the promoter activity of P2 in Hep3B cells. The purified protein also bound to the nuclear factor 1 (NF1)/CTF consensus sequence, albeit with lower affinity. Gel mobility supershift and Western blotting (immunoblotting) analyses using an antibody against the NF1/CTF protein identified the purified 32-and 34-kDa polypeptides as NF1 or a related protein. Cotransfection into Hep3B cells or primary rat hepatocytes of cDNAs of the NF1-like proteins NF1/L, NF1/X, and NF1/Red1 resulted in a three-to fivefold increase in transcription directed by wild-type P2 but not by the mutated P2. Partial hepatectomy markedly decreased the levels of NF1 in the remnant liver and its binding to P2, which paralleled declines in the rate of transcription of the ␣ 1b AR gene and in the steady-state levels of its mRNA. These observations indicate that NF1 activates transcription of the rat ␣ 1b AR gene via interacting with its P2 promoter and that a decline in the expression of NF1 is one of the mechanisms responsible for the reduced expression of the ␣ 1b AR gene during liver regeneration.The ␣ 1b adrenergic receptor (␣ 1b AR) is a G-protein-coupled receptor that mediates the acute metabolic effects of catecholamines in the liver and is also involved as a comitogen in the regenerative response after the loss or injury of liver tissue (13,14,35,36). Expression of the ␣ 1b AR gene in the rat liver is controlled by hormonal and developmental factors as well as by conditions associated with hepatocyte dedifferentiation (8,33,35,36,49). Such regulation has been shown to occur at the transcriptional level under many of these conditions, such as after partial hepatectomy (36), during primary culturing of rat hepatocytes (28), and in response to glucocorticoids (53), cycloheximide (25), phorbol esters (26), and thyrotropin and cyclic AMP (31). In order to understand the molecular mechanisms involved in the transcription of the ␣ 1b AR gene under a variety of physiological and pathological conditions, we have cloned the rat ␣ 1b AR gene and identified its multiple promoters and the cis-acting elements in its regulatory domai...

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confidence: 92%

Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver

Gao

Liu

Kunos

1996

Molecular and Cellular Biology

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“…If cells are damaged, profilin leaks from the cytoplasma into the extra-cellular space, then binds with a cell surface profilin receptor which translocates protein kinase C, and activates c-jun for TPA responsive element binding protein (TREB) formation. DNA binding protein TREB binds to TRE sites on promoters 11) , one of which is the b-FGF (fibroblast growth factor) gene 12) that induces fibroblast proliferation resulting in fibrosis 13,14) . The increase in mRNA for profilin indicates that mechanisms of phagocytosis of volcanic ash cause molecular changes in the lung that might be involved in triggering cell proliferation.…”

Section: Fig 11 Detection Of C-jun Mrna Using Reverse Transcriptionmentioning

confidence: 99%

“…AP-1 responsive element carrying gene b-EGF (fibroblastic growth factor) is expressed and elevates DNA synthesis. Thus, by affecting cell growth, profilin is involved in the progression of proliferation disease such as proliferative glomerulonephritis, carbon-tetra-chloride induced liver cirrhosis and alcoholic liver fibrosis [11][12][13][14] . To investigate the actions of profilin-induced c-jun gene expression in rat lungs after exposure to volcanic ash and SO 2 for 5 days, we determined phagocytosis in alveolar macrophages, expression of c-jun and profilin mRNAs, and production of TNF-α.…”

Section: Introductionmentioning

confidence: 99%

c-jun mRNA Expression and Profilin mRNA Amplification in Rat Alveolar Macrophages Exposed to Volcanic Ash and Sulfur Dioxide

et al. 2003

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Local residents exposed to heavy falls of ash discharged by Mt. Sakurajima, an active volcano, have been reported to develop acute and chronic inflammation of the respiratory tract. The present study aimed to determine the primary cause of this inflammation using an experimental model. Wistar rats were exposed for 5 days (4 h/d) to air containing 100 mg/m 3 volcanic ash (mass median aerodynamic diameter, 4.3 µm; geometric standard deviation, 1.7) with or without 1.5 ppm sulfur dioxide (SO 2 ). The lungs were then lavaged, and mRNA was extracted from alveolar macrophages and assessed by reverse transcription-polymerase chain reaction (RT-PCR). In the lavage fluid, no change in cellularity or increase in the content of tumor necrosis factor (TNF)-α α α α α was detected. However, at 1 h following exposure, 80% of macrophages were seen to have phagocytosed the volcanic ash. This percentage was unchanged at 24 h after exposure. Profilin mRNA content of the macrophages was elevated, and c-jun mRNA was expressed. Alveolar macrophages exposed to volcanic ash and SO 2 , therefore, are likely to have some inflammatory and fibrogenic potential.

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“…To date, a potential role of PFN1 in early liver cancer progression has not been reported. However, the upregulation of PFN1 is known to be positively associated with cell cycle progression by promoting liver regeneration upon hepatectomy [55]. Here, its downregulation may suggest an inhibitory effect of antibiotics on cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

Depletion of Gram-Positive Bacteria Impacts Hepatic Biological Functions During the Light Phase

Ellero‐Simatos

Manickam

et al. 2019

IJMS

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Living organisms display internal biological rhythms, which are an evolutionarily conserved adaptation to the environment that drives their rhythmic behavioral and physiological activities. The gut microbiota has been proposed, in association with diet, to regulate the intestinal peripheral clock. However, the effect of gut dysbiosis on liver remains elusive, despite that germfree mice show alterations in liver metabolic functions and the hepatic daily rhythm. We analyzed whether the disruption of gut microbial populations with various antibiotics would differentially impact liver functions in mice. Our results support the notion of an impact on the hepatic biological rhythm by gram-positive bacteria. In addition, we provide evidence for differential roles of gut microbiota spectra in xenobiotic metabolism that could protect against the harmful pharmacological effects of drugs. Our results underscore a possible link between liver cell proliferation and gram-positive bacteria.

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Increase of Hepatic mRNAs of Profilin, Actin and Extracellular Matrix Proteins After Carbon Tetrachloride Treatment and Partial Hepatectomy in Rats

Cited by 15 publications

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Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver

Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver

c-jun mRNA Expression and Profilin mRNA Amplification in Rat Alveolar Macrophages Exposed to Volcanic Ash and Sulfur Dioxide

Depletion of Gram-Positive Bacteria Impacts Hepatic Biological Functions During the Light Phase

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Increase of Hepatic mRNAs of Profilin, Actin and Extracellular Matrix Proteins After Carbon Tetrachloride Treatment and Partial Hepatectomy in Rats

Cited by 15 publications

References 0 publications

Transcriptional Regulation of α1b Adrenergic Receptors (α1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α1bAR Gene Expression in Regenerating Liver

Transcriptional Regulation of α1b Adrenergic Receptors (α1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α1bAR Gene Expression in Regenerating Liver

c-jun mRNA Expression and Profilin mRNA Amplification in Rat Alveolar Macrophages Exposed to Volcanic Ash and Sulfur Dioxide

Depletion of Gram-Positive Bacteria Impacts Hepatic Biological Functions During the Light Phase

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Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver

Transcriptional Regulation of α_1b Adrenergic Receptors (α_1bAR) by Nuclear Factor 1 (NF1): a Decline in the Concentration of NF1 Correlates with the Downregulation of α_1bAR Gene Expression in Regenerating Liver