Lung cancer is clearly the primary cause of cancer-related deaths worldwide. Recent molecular-targeted strategy has contributed to improvement of the curative effect of adenocarcinoma of the lung. However, such current treatment has not been developed for squamous cell carcinoma (SCC) of the disease. The new genome-wide RNA analysis of lung-SCC may provide new avenues for research and the development of the disease. Our recent microRNA (miRNA) expression signatures of lung-SCC revealed that clustered miRNAs miR-1/133a were significantly reduced in cancer tissues. Here, we found that restoration of both mature miR-1 and miR-133a significantly inhibited cancer cell proliferation, migration and invasion. Coronin-1C (CORO1C) was a common target gene of the miR-1/133a cluster, as shown by the genome-wide gene expression analysis and the luciferase reporter assay. Silencing of CORO1C gene expression inhibited cancer cell proliferation, migration and invasion. Furthermore, CORO1C-regulated molecular pathways were categorized by using si-CORO1C transfectants. Further analysis of novel cancer signaling pathways modulated by the tumor-suppressive cluster miR-1/133a will provide insights into the molecular mechanisms of lung-SCC oncogenesis and metastasis.
The development of targeted molecular therapies has greatly benefited patients with lung adenocarcinomas. In contrast, these treatments have had little benefit in the management of lung squamous cell carcinoma (lung SCC). Therefore, new treatment options based on current genomic approaches are needed for lung SCC. Aberrant microRNA (miRNA) expression has been shown to promote lung cancer development and aggressiveness. Downregulation of microRNA-218 (miR-218) was frequently observed in our miRNA expression signatures of cancers, and previous studies have shown an antitumor function of miR-218 in several types of cancers. However, the impact of miR-218 on lung SCC is still ambiguous. The present study investigated the antitumor roles of miR-218 in lung SCC to identify the target genes regulated by this miRNA. Ectopic expression of miR-218 greatly inhibited cancer cell migration and invasion in the lung SCC cell lines EBC-1 and SK-MES-1. Through a combination of in silico analysis and gene expression data searching, tumor protein D52 (TPD52) was selected as a putative target of miR-218 regulation. Moreover, direct binding of miR-218 to the 3′-UTR of TPD52 was observed by dual luciferase reporter assay. Overexpression of TPD52 was observed in lung SCC clinical specimens, and knockdown of TPD52 significantly suppressed cancer cell migration and invasion in lung SCC cell lines. Furthermore, the downstream pathways mediated by TPD52 involved critical regulators of genomic stability and mitotic checkpoint genes. Taken together, our data showed that downregulation of miR-218 enhances overexpression of TPD52 in lung SCC cells, promoting cancer cell aggressiveness. Identification of tumor-suppressive miRNA-mediated RNA networks of lung SCC will provide new insights into the potential mechanisms of the molecular pathogenesis of the disease.
The COPD-PS appears to be an adequate measure for large scale screening of possible airflow obstruction requiring further testing with spirometry.
Objective Obstructive sleep apnea syndrome (OSAS) is associated with increased cardiovascular morbidity and mortality. We investigated the values of brachialankle pulse wave velocity as an indicator of atherosclerosis in obstructive sleep apnea syndrome patients.Materials and Methods Brachial-ankle pulse wave velocity (baPWV) was measured in 104 OSAS patients and 104 healthy control subjects matched for age, sex, and body mass index (BMI). BaPWV values were compared in both groups and investigated with respect to the number of risk factors for atherosclerosis, including hypertension, hypercholesterolemia, impaired glucose tolerance, smoking, and obesity. Comparisons were also made between 48 OSAS group cases and 90 control group cases free from hypertension, which has a major impact on baPWV.Results As compared to the control group, the OSAS group had significantly higher baPWV (1,645±349 cm/s vs 1,436±278 cm/s, p<0.0001), and values obtained for baPWV were significantly higher in the OSAS group than in the control group even in groups free from hypertension (1,453±216 cm/s vs 1,374±213 cm/s, p<0.05). In both groups, baPWV rose as the number of risk factors for atherosclerosis increased, but baPWV was higher in the OSAS group than in the control group even in a comparison of individuals entirely free from risk factors (1,400±200 cm/s vs 1,198±79 cm/s, p<0.05).Conclusion The condition of OSAS itself is considered a possible risk factor for atherosclerosis. We believe that the usefulness of baPWV as an index of atherosclerosis merits further study in the frequently observed cases of OSAS complicated by cardiovascular disease. IntroductionObstructive sleep apnea syndrome (OSAS) is linked with increased hypertension, ischemic heart disease, heart failure, and strokes (1). However, there are few studies concerning atherosclerosis in OSAS patients. Pulse wave velocity (PWV) is known to be an indicator of arterial stiffness (2, 3), and has been regarded as a marker reflecting vascular damage (4, 5). Although recently a simple non-invasive automatic measurement of brachial-ankle PWV (baPWV) has been described and the validity and reproducibility of this new technique have been reported (6), there have been no studies to assess the significance of baPWV in OSAS patients.In this study the OSAS group and healthy control group were compared with respect to complications of atherosclerotic risk factors such as hypertension, obesity, hypercholesterolemia, smoking, and impaired glucose tolerance. Also the values of baPWV as an indicator of atherosclerosis were compared in the OSAS group and healthy control group, and the significance of these atherosclerotic risk factors on baPWV was verified in the OSAS group. Materials and Methods Study subjectsSubjects were 104 OSAS patients (92 men and 12 women, mean age, 53.3±9.5 years) examined in our department from
BackgroundNapsin A, an aspartic protease, is mainly expressed in alveolar type-II cells and renal proximal tubules and is a putative immunohistochemical marker for pulmonary adenocarcinomas. This study sought to determine whether napsin A could be measured in the serum to evaluate its relationship to idiopathic pulmonary fibrosis (IPF) and determine whether renal dysfunction might affect serum napsin A levels.MethodsSerum levels of napsin A were measured in 20 patients with IPF, 34 patients with lung primary adenocarcinoma, 12 patients with kidney diseases, and 20 healthy volunteers. Surfactant protein (SP)-A, SP-D, and Krebs von den Lungen-6 (KL-6) levels in serum and pulmonary function tests were also evaluated in IPF patients.ResultsCirculating levels of napsin A were increased in patients with IPF, as compared with healthy controls, and they correlated with the severity of disease. Moreover, the serum napsin A levels were not elevated in patients with pulmonary adenocarcinoma or renal dysfunction. The distinguishing point between IPF and the controls was that the area under the receiver operating characteristic curve (ROC) of napsin A was larger than that of KL-6, SP-A, or SP-D.ConclusionThese findings suggest that serum napsin A may be a candidate biomarker for IPF.
BackgroundThe incidence of chronic obstructive pulmonary disease (COPD) is increasing worldwide. In Japan and other countries, epidemiological studies have found that many patients with COPD are underdiagnosed and untreated, and thus, early detection and treatment of COPD has been emphasized. Screening questionnaires may have utility in the initial detection of COPD.ObjectiveThis study aimed to validate and compare the COPD Population Screener (COPD-PS) and the International Primary Care Airway Group (IPAG) questionnaires in a general Japanese population.Patients and methodsEligible subjects 40 years of age and older living in the town of Hisayama were solicited to participate in a health checkup in 2012. All subjects 40–79 years of age without physician-diagnosed asthma or lung resection were recruited, and 2,336 subjects who fully completed both questionnaires and who had valid spirometry measurements were analyzed. Persistent airflow obstruction (AO) was defined by a postbronchodilator forced expiratory volume in 1 second/forced vital capacity <0.70. Receiver operating characteristic curves, net reclassification improvement, and integrated discrimination improvement were used to examine the ability of the COPD-PS and IPAG questionnaires to discriminate between subjects with and without AO.ResultsThe overall area under the receiver operating characteristic curve for the COPD-PS questionnaire was 0.747 (95% confidence interval [CI], 0.707–0.788) and for the IPAG was 0.775 (95% CI, 0.735–0.816), with no significant difference (P=0.09). The net reclassification improvement and integrated discrimination improvement were −0.107 (95% CI, −0.273–0.058; P=0.203) and −0.014 (95% CI, −0.033–0.006; P=0.182), respectively.ConclusionThe five-item COPD-PS questionnaire was comparable to the eight-item IPAG for discriminating between subjects with and without AO. The COPD-PS is a simple and useful screening questionnaire for persistent AO.
Angiopoietin-like protein 2 (ANGPTL2) is a chronic inflammatory mediator that, when deregulated, is associated with various pathologies. However, little is known about its activity in lung. To assess a possible lung function, we generated a rabbit monoclonal antibody that specifically recognizes mouse ANGPTL2 and then evaluated protein expression in mouse lung tissue. We observed abundant ANGPTL2 expression in both alveolar epithelial type I and type II cells and in resident alveolar macrophages under normal conditions. To assess ANGPTL2 function, we compared lung phenotypes in Angptl2 knockout (KO) and wild-type mice but observed no overt changes. We then generated a bleomycin-induced interstitial pneumonia model using wild-type and Angptl2 KO mice. Bleomycin-treated wild-type mice showed specifically upregulated ANGPTL2 expression in areas of severe fibrosing interstitial pneumonia, while Angptl2 KO mice developed more severe lung fibrosis than did comparably treated wild-type mice. Lung fibrosis seen following bone marrow transplant was comparable in wild-type or Angptl2 KO mice treated with bleomycin, suggesting that Angptl2 loss in myeloid cells does not underlie fibrotic phenotypes. We conclude that Angptl2 deficiency in lung epithelial cells and resident alveolar macrophages causes severe lung fibrosis seen following bleomycin treatment, suggesting that ANGPTL2 derived from these cell types plays a protective role against fibrosis in lung.
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