Increase in the detection sensitivity of a lateral flow assay for a cardiac marker by oriented immobilization of antibody

Ryu, Yiseul; Jin, Zongwen; Kang, Mun Suk; Kim, Kwang Ho

doi:10.1007/s13206-011-5301-2

Cited by 50 publications

(23 citation statements)

References 16 publications

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“…In addition to MAR, other technologies like the use of giant magnetoresistive sensors (GMR) are emerging (Taton et al, 2009;Ryu et al, 2011;Marquina et al, 2012), GMR signaling is produced when the electrical resistance of the sensor is reduced by the effect of an external magnetic field derived from the MNPs. The technique can show a quite low limit of detection (12 pg/mL; Taton et al, 2009) but seems that its robustness and sensitivity still have to be improved.…”

Section: Magnetic Methodsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

495

293

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Lateral flow biosensors (LFBs) are paper-based devices which permit the performance of low-cost and fast diagnostics with good robustness, specificity, sensitivity and low limits of detection. The use of nanoparticles (NPs) as labels play an important role in the design and fabrication of a lateral flow strip (LFS). The choice of NPs and the corresponding detection method directly affect the performance of these devices. This review discusses aspects related to the application of different nanomaterials (e.g. gold nanoparticles, carbon nanotubes, quantum dots, up-converting phosphor technologies, and latex beads, between others) in LFBs. Moreover, different detection methods (colorimetric, fluorescent, electrochemical, magnetic, etc.) and signal enhancement strategies (affording secondary reactions or modifying the architecture of the LFS) as well as the use of devices such as smartphones to mediate the response of LFSs will be analyzed.

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Section: Magnetic Methodsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

495

293

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“…A layer of anti-mouse antibody was absorbed on the conjugate to ensure the correct orientation of the capture antibody on the PMPs. Previous work has shown this to increase the detection sensitivity of lateral flow assays [ 19 ]. A schematic diagram of the design of the lateral flow assay for total PSA using monoclonal antibodies is shown in Figure 2 .…”

Section: Methodsmentioning

confidence: 99%

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

et al. 2014

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We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.

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“…However, up to 40% of misdiagnosed myocardial infarction (MI) cases occur from misinterpretation of electrocardiogram. Thus, levels of cardiac biochemical markers such as cTnI or cTnT, CK‐MB, and myoglobin are measured to rule out MI . But, routine checking of these biomarkers shows limited sensitivity for early detection of MI.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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Increase in the detection sensitivity of a lateral flow assay for a cardiac marker by oriented immobilization of antibody

Cited by 50 publications

References 16 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

Recent applications of paper‐based point‐of‐care devices for biomarker detection

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