An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

Barnett, Jacqueline M.; Wraith, Patrick; Kiely, Janice; Persad, Raj; Hurley, Katrina; Hawkins, Peter; Luxton, Richard

doi:10.3390/bios4030204

Cited by 47 publications

(25 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wang et al (2009), Zheng et al (2012), D.B. Wang et al (2013, Shi et al (2015b), Barnett et al (2014) and Wang et al (2015) reported the use of MAR on their works, obtaining wide working ranges, high selectivity, robust assays, good sensitivity and lower limits of detection than standard AuNPs LFs. However, the intensity of the signal was related to the size of the MNPs, being the larger ones which produced higher responses being this a drawback inasmuch as it slows significantly the assay time.…”

Section: Magnetic Methodsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

497

293

View full text Add to dashboard Cite

Lateral flow biosensors (LFBs) are paper-based devices which permit the performance of low-cost and fast diagnostics with good robustness, specificity, sensitivity and low limits of detection. The use of nanoparticles (NPs) as labels play an important role in the design and fabrication of a lateral flow strip (LFS). The choice of NPs and the corresponding detection method directly affect the performance of these devices. This review discusses aspects related to the application of different nanomaterials (e.g. gold nanoparticles, carbon nanotubes, quantum dots, up-converting phosphor technologies, and latex beads, between others) in LFBs. Moreover, different detection methods (colorimetric, fluorescent, electrochemical, magnetic, etc.) and signal enhancement strategies (affording secondary reactions or modifying the architecture of the LFS) as well as the use of devices such as smartphones to mediate the response of LFSs will be analyzed.

show abstract

Section: Magnetic Methodsmentioning

confidence: 99%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

497

293

View full text Add to dashboard Cite

show abstract

“…Biosensors based on microfabricated tin-film electrochemical sensor [31] allow detection down to a concentration of 0.12 ng mL −1 , compared with this detection method, the separable detection method using micro fluxgate assay allows samples to be conveniently and quickly changed. Due to the PSA capturing was achieved on the small glass surface; in comparison to the other biosensors [32,33] such as GMR [34] that the assay 's surface need functionalization, this assay was not contacting and immersing in chemical and bio solutions. So, the sensor can avoid being contaminated by those solutions and keep excellent stability for reuse.…”

Section: Detection Of Psa Labeled With Dynabeadsmentioning

confidence: 99%

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

et al. 2016

View full text Add to dashboard Cite

We describe a micro fluxgate based device with rectangular magnetic core for the determination of prostate specific antigen (PSA) labeled with Dynabeads. A sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. The secondary antibody is labeled with Dynabeads. By applying a DC magnetic fields in the range of 460 to 700 μT, PSA can be detected with detection limit as low as 0.1 ng mL −1 . This micro fluxgate-based assay offers the advantages of miniaturization, simple and conveniently manipulation, reusability and stability. In our perception, it offers a viable approach towards clinical determination of PSA or other biomarkers.

show abstract

“…Zao et al [119] improved the detection limit by two orders of magnitude for photoacoustic analysis compared to colorimetric measurements. The magnetic properties of the nanodispersed label in LFIA were recorded by Barnett et al [120], Chen et al [121], Lago-Cachon et al [122], and other authors. Several variants of LFIA with electrochemical detection are presented in the literature, the most recent of which (the work of Zhao et al [123]) is based on the use of a serial glucometer as a registrar.…”

Section: Proper Response For Lfiamentioning

confidence: 89%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

View full text Add to dashboard Cite

This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

show abstract

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

Cited by 47 publications

References 33 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Contact Info

Product

Resources

About