Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Sun, Xuecheng; Lei, Chong; Guo, Lei; Zhou, Yong

doi:10.1007/s00604-016-1889-1

Cited by 18 publications

(13 citation statements)

References 34 publications

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“…Although those methods are reliable, most of them require long measurement procedures, high costs, complex equipment, highly qualified personnel and are not appropriate for massive decentralized assays. Therefore, nowadays it is still a challenge to develop simple, low‐cost and appropriate detection methods for sensitive determination of PSA levels . In this work, a simple and sensitive nanostructured label free immunosensor for PSA quantification is proposed.…”

Section: Introductionsupporting

confidence: 46%

“…Blood PSA concentration is significantly augmented in prostate cancer patients, because of the disorder of the prostate gland . PSA is used extensively as a prostate cancer biomarker with the aim of screen, diagnose, follow‐up the response to treatment and detection of recurrence after definite therapy . A value of PSA higher than the cut‐off value (2.5–4 ng mL −1 ) indicates high probability of prostate cancer .…”

Section: Introductionmentioning

confidence: 99%

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Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

et al. 2018

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This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.

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Section: Introductionsupporting

confidence: 46%

Section: Introductionmentioning

confidence: 99%

Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

et al. 2018

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“…Therefore, some welldeveloped methods for magnetic¯eld detection have been used in biosensing applications, including (i) magnetoresistive (MR) methods such as anisotropic magnetoresistance (AMR), [46][47][48][49][50][51][52] giant magnetoresistance (GMR), [53][54][55][56][57] tunneling magnetoresistance (TMR), 58,59 giant magnetoimpedance (GMI) [60][61][62][63] and (ii) nonMR methods such as those involving the use of a°ux gate sensor, Hall sensor and superconducting quantum interference device (SQUID). [64][65][66][67][68][69] Several magnetic¯eld sensors have been used in biodiagnostics, but the drawbacks of large size, low sensitivity, or high power consumption limited their use in practical applications. 70 The use of magnetic sensors based on MR e®ects is a promising alternative to overcoming these concerns related to nonMR sensors.…”

Section: Magnetic Sensing Methodsmentioning

confidence: 99%

“…For example, the micro°uxgate sensor is used to trap and detect speci¯c antigens. 69 When a uniform magnetic¯eld is applied in the out-of-plane direction of a°at rectangular semiconductor and in which electric current°ows along the long bar, voltage is then generated across the width of the°at plate, as shown in Fig. 6(a).…”

Section: Magnetic Biosensors Other Than Mr Biosensorsmentioning

confidence: 99%

Magnetoresistive Biosensors for Direct Detection of Magnetic Nanoparticle Conjugated Biomarkers on a Chip

Huang

Garu

et al. 2019

SPIN

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In this review, we introduce various magnetic biosensors that have been developed. We first explain the advantages of magnetic biosensing and their general operating principles as well as the biolabeling technique for magnetic nanoparticles. Next, we focus on magnetoresistive biosensing technologies because magnetoresistive biosensors will be an essential development direction due to the demand for miniaturization and portable lab-on-a-chip devices. The magnetoresistive effects employed in biosensing include anisotropic magnetoresistance, giant magnetoresistance and tunneling magnetoresistance. In addition to magnetoresistive sensors, the advantages and disadvantages of some nonmagnetoresistive magnetic biosensors are discussed and compared. Finally, we introduce research on integrating magnetic biosensors into the microfluidic laboratory-on-a-chip systems and comment on future development trends.

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“…A completely different approach for PSA biosensing was described by Sun et al by exploiting magnetic-based biosensors [153]. They used a micro-fluxgate based device with a rectangular magnetic core for the determination of PSA labelled with Dynabeads.…”

Section: Piezoelectric and Other Alternative Methods For Psa Detectionmentioning

confidence: 99%

Nanomaterial-based biosensors for detection of prostate specific antigen

et al. 2017

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Screening serum for the presence of prostate specific antigen (PSA) belongs to the most common approach for the detection of prostate cancer. This review (with 156 refs.) addresses recent developments in PSA detection based on the use of various kinds of nanomaterials. It starts with an introduction into the field, the significance of testing for PSA, and on current limitations. A first main section treats electrochemical biosensors for PSA, with subsections on methods based on the use of gold electrodes, graphene or graphene-oxide, carbon nanotubes, hybrid nanoparticles, and other types of nanoparticles. It also covers electrochemical methods based on the enzyme-like activity of PSA, on DNA-, aptamer- and biofuel cell-based methods, and on the detection of PSA via its glycan part. The next main section covers optical biosensors, with subsections on methods making use of surface plasmon resonance (SPR), localized SPR and plasmonic ELISA-like schemes. This is followed by subsections on methods based on the use of fiber optics, fluorescence, chemiluminescence, Raman scattering and SERS, electrochemiluminescence and cantilever-based methods. The most sensitive biosensors are the electrochemical ones, with lowest limits of detection (down to attomolar concentrations), followed by mass cantilever sensing and electrochemilumenescent strategies. Optical biosensors show lower performance, but are still more sensitive compared to standard ELISA. The most commonly applied nanomaterials are metal and carbon-based ones and their hybrid composites used for different amplification strategies. The most attractive sensing schemes are summarized in a Table. The review ends with a section on conclusions and perspectives.

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Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

Cited by 18 publications

References 34 publications

Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

Magnetoresistive Biosensors for Direct Detection of Magnetic Nanoparticle Conjugated Biomarkers on a Chip

Nanomaterial-based biosensors for detection of prostate specific antigen

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