Incorporation of Boron in Mesoporous Bioactive Glass Nanoparticles Reduces Inflammatory Response and Delays Osteogenic Differentiation

Abstract

Mesoporous bioactive glass nanoparticles (MBG) are multifunctional building blocks for tissue regeneration and nanomedicine applications. Incorporation of biologically active ions can endow MBG with additional functionalities toward promoted therapeutic effects. Here, boron is incorporated into MBG by using a sol–gel approach. The concentration of boron incorporated is controllable by tuning the amount of boron precursor. Two types of boron‐doped MBG, namely 10B‐ and 15B‐MBG (5.8 and 6.5 mol% of B2O3, respectively) are synthesized. Boron incorporation does not significantly influence the particle morphology. All synthesized particles exhibit a sphere‐like shape with a size ranging from 100 to 300 nm. 10B‐ and 15B‐MBG show large specific surface area (346 and 320 m² g−1, respectively) and pore volume. Both boron‐doped MBG exhibit remarkable in vitro bioactivity and noncytotoxicity. Boron incorporation is shown to reduce the inflammatory response linked to macrophages as indicated by downregulated expression of pro‐inflammatory genes. However, boron incorporation delays the osteogenic differentiation in osteoblasts as indicated by the downregulated expression of pro‐osteogenic genes. The results demonstrate the promising potential of using boron‐doped MBG to modulate inflammatory response for bone regeneration under inflammatory conditions, as shown in this study for the first time.

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

“…The new diffraction signals of the XRD pattern ( Figure 6 b) were detected at approximate by 2θ = 26.0°, 31.7°, 32.1°, 32.9°, and 49.4°, which correspond to hydroxyapatite (JCPD 09-0432) [ 80 ]. Besides, with the observation of surface morphology ( Figure 6 c), needle-like structures were seen to be present on the surface of PHBV/MBGN/CIN20 microspheres, which exhibit the characteristic morphology of HA formed on MBGN after immersing in SBF [ 70 , 81 ]. Further verification of the presence of HA was performed by EDS analysis ( Figure 6 d).…”

Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

“…There are reports in the literature on the fabrication of B-doped bioactive glass nanoparticles [ 49 , 50 ], but, to the best of our knowledge, there are very limited data available on the fabrication and characterization of Co-doped B-containing bioactive glass nanoparticles. Rad et al reported that the content of B in their B-doped bioactive glass nanoparticles, prepared using an acid-base catalyzed sol-gel method, was quite similar to their designed compositions [ 49 ].…”

Ag–Sr doped mesoporous bioactive glass nanoparticles loaded chitosan/gelatin coating for orthopedic implants

“…13,14 MBGNs can be doped with several metallic ions to provide a therapeutic effect. 15,16 For example, Westhauser et al 17 and Nawaz et al 18 doped MBGNs (composition in mol.%: 50 SiO 2 -10 P2O 5 -40 Cao) with Mn (composition in mol.%: 50 SiO 2 -10 P2O 5 -35 Cao-5 MnO). The results have shown that the 5 mol.% addition of MnO in the MBGNs supported osteogenic differentiation and enhanced the upregulation of genes encoding for extracellular matrix proteins.…”

“…MBGNs based on SiO 2 –P2O 5 –Cao have demonstrated promising properties for the local delivery of therapeutically active ions 13,14 . MBGNs can be doped with several metallic ions to provide a therapeutic effect 15,16 . For example, Westhauser et al 17 and Nawaz et al 18 doped MBGNs (composition in mol.%: 50 SiO 2 –10 P2O 5 –40 Cao) with Mn (composition in mol.%: 50 SiO 2 –10 P2O 5 –35 Cao–5 MnO).…”