In this study, we investigated surface and biological properties of Ag-Sr-doped mesoporous bioactive glass nanoparticle (Ag-Sr MBGN) loaded chitosan/gelatin coatings deposited by electrophoretic deposition (EPD) on 316L stainless steel.The EPD parameters, that is, deposition time, applied voltage, and distance between the electrodes was optimized by the Taguchi design of experiment (DoE) approach.Scanning electron microscopy (SEM) images illustrated the spherical morphology of the synthesized Ag-Sr MBGNs with the mean particle size of 160 ± 20 nm. Energydispersive X-ray (EDX) spectroscopy results confirmed the presence of Ag and Sr in the synthesized MBGNs. Optimum EPD parameters determined by DoE approach were 5 g/L of Ag-Sr MBGNs, deposition time of 5 min, and applied voltage of 30 V. SEM images confirmed that the coatings were fairly homogenous. Fourier-transform infrared spectroscopy and EDX results confirmed the presence of chitosan, gelatin, and Ag-Sr MBGNs in the coatings. Chitosan/gelatin/Ag-Sr MBGN composite coatings exhibited suitable wettability for the protein attachment and proliferation of osteoblast cells. The composite coatings exhibited suitable adhesion strength with the substrate. The coatings developed HA crystals upon immersion in simulated body fluid. The results of the turbidity test confirmed that the coatings are antibacterial to the Escherichia coli cells.