Incorporation of BMP-2 nanoparticles on the surface of a 3D-printed hydroxyapatite scaffold using an ε-polycaprolactone polymer emulsion coating method for bone tissue engineering

Kim, Beom-Su; Yang, Sun-Sik; Kim, Cheol Sang

doi:10.1016/j.colsurfb.2018.06.043

Cited by 80 publications

(62 citation statements)

References 40 publications

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“…The release of BMP-2 corresponded to the kinetic characteristics, which showed that a suitable carrier was important. Kim et al 31 cross-linked BMP-2 on hydroxylapatite and PCL scaffolds to repair rabbit skull defects; however, the PCL polymer was difficult to degrade, which affected the formation of new bone, and the material was not suitable for arthroscopic surgery. Encapsulation of BMP-2 in microspheres is a common method of delivery.…”

Section: Discussionmentioning

confidence: 99%

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Wu¹,

Gao²,

Xu³

2020

IJN

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Purpose Developmental dysplasia of the hip (DDH) can increase the pressure between the joints, which causes secondary hip osteoarthritis. The aim of the present study was to fabricate poly(D, L-lactic acid)-poly(ethylene glycol)-poly(D, L-lactic acid) (PELA) electrospun fibrous scaffolds, immobilized with bone morphogenetic protein-2 (BMP-2), to repair the acetabulum defects. Methods The characteristics of PELA electrospun were analyzed using scanning electron microscopy, the release kinetics of BMP-2 in vitro were analyzed using enzyme-linked immunosorbent assays. Human mesenchymal stem cells (hMSCs) were used for in vitro experiments, the biocompatibility of the electrospinning materials was investigated using a cell counting kit-8 (CCK-8) kit, and osteogenic differentiation was tested via alkaline phosphatase (ALP) activity and relative gene expression. Eighteen miniature pig animal models were used in the in vivo experiment. The pigs were sacrificed at 24 weeks after surgery, and the reconstructed acetabulum was evaluated using histological sections. Results Structural analysis revealed that the diameter of the PELA electrospun fiber was 0.8195 ± 0.16 μm. The PELA electrospun fiber materials showed good hydrophilicity and biocompatibility and could continuously release BMP-2 within 27 days: 16.07 ± 0.11 ng of BMP-2 was released from the scaffold. A total of sixteen implants fully filled the defects, and hematoxylin and eosin staining and Goldner’s trichrome staining showed that the simple tendon group (T group) was mostly fibrous tissues, lots of fibroblasts and small amounts of chondrocytes were observed in the polydopamine-coated electrospun fiber group (DP group). The DP plus BMP-2 (DPB) group showed a large number of chondrocytes and partial new bone tissues. Conclusion PELA electrospun fibrous scaffolds are good sustained-release carriers, which can not only induce implant differentiation into cartilage and bone but also are completely degraded without toxicity. Therefore, the material can be used for bone and cartilage regeneration.

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Section: Discussionmentioning

confidence: 99%

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Wu¹,

Gao²,

Xu³

2020

IJN

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“…On the other hand, related to enhancement of bone repair/regeneration processes, growth factors incorporation into the polymeric top layer could be investigated. As such, in ref . 3D printed HA was coated with PCL with BMP‐2‐loaded nanoparticles (PLGA and poly(vinyl alcohol)) resulting in optimal cellular cytocompatibility, no cytotoxicity and improved bone regeneration in a rabbit calvarial defect model.…”

Section: Discussionmentioning

confidence: 99%

Hierarchical Functionalized Polymeric‐Ceramic Coatings on Mg‐Ca Alloys for Biodegradable Implant Applications

Santos-Coquillat

Martínez-Campos

Vargas-Alfredo

et al. 2019

Macromolecular Bioscience

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/mabi.201900179. Hierarchical BiomaterialMagnesium-based implants present several advantages for clinical applications, in particular due to their biocompatibility and degradability. However, degradation products can affect negatively the cell activity. In this work, a combined coating strategy to control the implant degradation and cell regulation processes is evaluated, including plasma electrolytic oxidation (PEO) that produces a 13 µm-thick Ca, P, and Si containing ceramic coating with surface porosity, and breath figures (BF) approach that produces a porous polymeric poly(ε-caprolactone) surface. The degradation of PCL-PEO-coated Mg hierarchical scaffold can be tailored to promote cell adhesion and proliferation into the porous structure. As a result, cell culture can colonize the inner PEOceramic coating structure where higher amount of bioelements are present. The Mg/PEO/PCL/BF scaffolds exhibit equally good or better premyoblast cell adhesion and proliferation compared with Ti CP control. The biological behavior of this new hierarchical functionalized scaffold can improve the implantation success in bone and cardiovascular clinical applications.

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“…The embedding of CQ‐PCL NPs in the CS/Col/HA scaffolds also modified the microenvironment of the particles, which resulted in significant prolonged release of CQ . These results indicated that the scaffolds loaded with CQ‐PCL NPs had a double sustained mechanism for drug release . In addition, the direct blending of CQ extract into polymeric scaffold resulted in the changes of scaffold properties, which are porosity, degradation rate, water adsorption as well as might change cell‐material interaction.…”

Section: Resultsmentioning

confidence: 96%

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

Thongtham

Chai‐in

Unger

et al. 2020

Polymers for Advanced Techs

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Here, we demonstrated the fabrication of a composite scaffold (chitosan [CS], collagen [Col], and hydroxyapatite [HA]) with the incorporation of encapsulated Cissus quadrangularis (CQ) extract for tissue engineering applications. First, the crude extract of CQ loaded nanoparticles were synthesized via double emulsion technique using polycaprolactone (PCL) and polyvinyl alcohol (PVA) as oil and aqueous phases, respectively. Both PCL (20, 40, and 80 mg/mL) and PVA (0.5%, 1%, and 3% w/v) concentrations were varied to determine the optimum concentrations for CQ-loaded nanoparticle preparation. The CQ-loaded PCL nanoparticles (CQ-PCL NPs), prepared with 20 mg/mL PCL and 0.5% (w/v) PVA, exhibited the smallest size of 334.22 ± 43.21 nm with 95.54 ± 1.49% encapsulation efficiency. Then, the CQ-PCL NPs were incorporated into the CS/Col/HA scaffolds. These scaffolds were also studied for their ultrastructure, pore sizes, chemical composition, compressive modulus, water swelling, weight loss, and biocompatibility. The results showed that the addition of CQ-PCL NPs into the scaffolds did not dramatically alter the ultrastructure and properties of the scaffolds, compared to CS/Col/HA scaffolds alone. However, incorporation of CQ-PCL NPs in the scaffolds improved the release profile of CQ by preventing the initial burst release and prolonging the release rate of CQ. In addition, the CQ-PCL NPs-loaded CS/Col/HA scaffolds supported the attachment and proliferation of MC3T3-E1 osteoblast cells.

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Incorporation of BMP-2 nanoparticles on the surface of a 3D-printed hydroxyapatite scaffold using an ε-polycaprolactone polymer emulsion coating method for bone tissue engineering

Cited by 80 publications

References 40 publications

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

<p>Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model</p>

Hierarchical Functionalized Polymeric‐Ceramic Coatings on Mg‐Ca Alloys for Biodegradable Implant Applications

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

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