Incorporating α-Allyl Glucoside into Polyacrylonitrile by Water-Phase Precipitation Copolymerization To Reduce Protein Adsorption and Cell Adhesion

Abstract

R-Allyl glucoside (AG) was incorporated into polyacrylonitrile by water-phase precipitation copolymerization (WPPCP) for the first time with K 2S2O8-Na2SO3 as initiator system to improve the resistance properties of protein adsorption and cell adhesion for acrylonitrile-based polymer. The effects of initiator concentration, reaction time and temperature, and total monomer concentration on the copolymerization were studied, and some results were compared with those of solution copolymerization using AIBN as initiator. FT-IR, 1 H NMR, and 13 C NMR spectroscopes, element analysis, and DSC measurement were used to characterize the copolymers. It was found that both the yield and molecular weight for the WPPCP were higher than those for solution polymerization. The AG content in the resulting copolymers and the AG conversion for WPPCP were also higher than those of solution polymerization. The surface properties of the carbohydrate-containing copolymers were studied by pure water contact angle, protein adsorption, and cell adhesion measurements. It was found that the contact angle of the copolymer films decreased from 68°to 30°with the increase of AG content in the copolymer. The adsorption amount of bovine serum albumin (BSA) and the adhesive number of macrophage on the film surface also decreased significantly with increasing R-allyl glucoside content from 0 to 42 wt % in the copolymer. These results revealed that both the hydrophilicity and biocompatibility of polyacrylonitrile-based membranes could be improved by copolymerization acrylonitrile with vinyl carbohydrates.

Fibrous Membranes Electrospinning from Acrylonitrile‐Based Polymers: Specific Absorption Behaviors and States of Water

“…[36][37][38][39][40] It is well known that PAN shows many advantages and has been widely used as membrane materials. [18,[41][42][43][44][45] According to our previous work and other reports, [1,20,46] poly[acrylonitrile-co-(N-vinyl-2-pyrrolidone)] (PANCNVP) is of excellent biocompatibility and shows some applications in blood-contacting devices and enzyme immobilization. Since the dense membranes have been studied previously, herein, the states of water in the corresponding fibrous membranes as well as the swelling behaviors are investigated.…”

Cytocompatibility of poly(acrylonitrile-co-N-vinyl-2-pyrrolidone) membranes with human endothelial cells and macrophages

“…The murine macrophage suspension was prepared using the method reported previously [17]. This suspension was isolated from freshly killed mice.…”

“…It is believed that the behavior of cells on a specific biomaterial surface may vary greatly with the type of cell investigated [15,16]. Macrophages are a kind of immune cell and perform various functions in living bodies, such as migration, phagocytosis, secretion, antigen presentation and survival through precisely modulated adhesion [17,18]. Preventing the activation of immune cells is a requirement for biomedical membranes.…”

Tethering poly(ethylene glycol)s to improve the surface biocompatibility of poly(acrylonitrile-co-maleic acid) asymmetric membranes

“…The murine macrophage suspension was prepared with the method reported previously [35]. The suspension was isolated from freshly killed mice using chloroform.…”