Tethering poly(ethylene glycol)s to improve the surface biocompatibility of poly(acrylonitrile-co-maleic acid) asymmetric membranes

Xu, Zhi‐Kang; Nie, Fu-Qiang; Qu, Chen; Wan, Ling‐Shu; Wu, Jian; Yao, Ke

doi:10.1016/j.biomaterials.2004.03.008

Cited by 81 publications

(41 citation statements)

References 36 publications

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“…The bioinertness of PEG provides a noncytotoxic environment that can be easily manipulated, sterilized, and used for cell culture. PEG has been shown to resist protein adhesion and cell attachment, [35][36][37][38] and this characteristic not only promotes cell-cell interactions over cell-material interactions, but also allows the facile removal of cell aggregates from microwell devices.…”

Section: Discussionmentioning

confidence: 99%

A Microwell Cell Culture Platform for the Aggregation of Pancreatic β-Cells

Bernard

Lin

Anseth

2012

Tissue Engineering Part C: Methods

118

138

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Cell-cell contact between pancreatic b-cells is important for maintaining survival and normal insulin secretion. Various techniques have been developed to promote cell-cell contact between b-cells, but a simple yet robust method that affords precise control over three-dimensional (3D) b-cell cluster size has not been demonstrated. To address this need, we developed a poly(ethylene glycol) (PEG) hydrogel microwell platform using photolithography. This microwell cell-culture platform promotes the formation of 3D b-cell aggregates of defined sizes from 25 to 210 mm in diameter. Using this platform, mouse insulinoma 6 (MIN6) b-cells formed aggregates with cell-cell adherin junctions. These naturally formed cell aggregates with controllable sizes can be removed from the microwells for macroencapsulation, implantation, or other biological assays. When removed and subsequently encapsulated in PEG hydrogels, the aggregated cell clusters demonstrated improved cellular viability ( > 90%) over 7 days in culture, while the b-cells encapsulated as single cells maintained only 20% viability. Aggregated MIN6 cells also exhibited more than fourfold higher insulin secretion in response to a glucose challenge compared with encapsulated single b-cells. Further, the cell aggregates stained positively for E-cadherin, indicative of the formation of cell junctions. Using this hydrogel microwell cell-culture method, viable and functional b-cell aggregates of specific sizes were created, providing a platform from which other biologically relevant questions may be answered.

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Section: Discussionmentioning

confidence: 99%

A Microwell Cell Culture Platform for the Aggregation of Pancreatic β-Cells

Bernard

Lin

Anseth

2012

Tissue Engineering Part C: Methods

118

138

View full text Add to dashboard Cite

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“…[31][32][33] Some researchers have been introduced polymers with photo-active or chemical-active into UF membranes though polymer blending. 26,28,34 Carboxyl groups have chemical reactivity and could be used to further modification. For example, Xu and coworkers 26,28 have synthesized poly(acrylonitrile-co-maleic acid)s (PANCMA) and blend it with polyacrylonitrile to fabricate UF membrane.…”

Section: Introductionmentioning

confidence: 99%

“…26 In recent years, the immobilization of PEG on material surface to improve the blood compatibility and minimize membrane fouling is well-documented in Refs. [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Tethering methoxy polyethylene glycols to improve the antifouling property of PSF/PAA‐blended membranes

Cao

Kang

et al. 2011

J of Applied Polymer Sci

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Poly(acrylic acid) (PAA) was used as an additive to fabricate blended polysulfone (PSF) ultrafiltration (UF) membranes. Hexanediamine was used as a crosslinking agent to react with PAA and formed an active surface with amine group. Then, an end carboxyl group methoxy polyethylene glycol (MPEG) was grafted on the membrane surface via an amidation reaction. Water contact angle measurement indicated that the surface hydrophilicity of PSF/PAA-blended membranes and MPEGmodified membranes remarkably increased. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR/FTIR) was used to confirm the existence of PAA in the blended membranes and the change of chemical composition. Membrane surface and cross-sectional morphologies were observed by scanning electron microscope. The flux of pure water increased slightly after modification, while the rejection to bovine serum albumin had no obvious change. The improvement of antifouling property for MPEG-modified membrane was accordant with the increase of PAA content in PSF/PAA-blended membranes. Protein UF experiments revealed that membrane fouling, especially irreversible membrane fouling, was remarkably reduced due to the incorporation of MPEG. The long-term protein UF experiment demonstrated the improvement of recycling property and the reliability of modification.

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“…Particle size has been shown to be a critical design parameter, as particles with diameters less than 200 nm and having PEG shells avoid entrapment by the reticuloendothelial system (RES) and accumulate preferentially in tumors that typically exhibit an enhanced permeability and retention effect (EPR). (5,15) The biodistribution and uptake by the tumor of the nanoparticle is further dictated by charge density, conformation, hydrophobicity, and immunogenicity. (16,17) The drug loading efficiency of the nanoparticles is also governed by a number of critical parameters, particularly the chemical affinity of the loaded drug for the nanoparticle core.…”

Section: Introduction Backgroundmentioning

confidence: 99%

Nanospheric Chemotherapeutic and Chemoprotective Agents

Sheihet¹

2008

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE (DD-MM-YYYY) ABSTRACTPurpose: Investigate the delivery of camptothecin in the presence of vitamin D3 with triblock copolymer-derived nanospheres that will increase the solubility of both of the drugs and provide protection to the camptothecin's lactone ring, resulting in its increased bioavailability to breast cancer cells. To date we have done the evaluation of two nanosphere formulations containing short alkyl pendent chain (DTB-SA/5K, Butyl) and/or benzyl ring (DTBn-SA/5K) Drug-binding efficiency of these nanospheres was evaluated for a constant quantity of the nanospheres with varying concentrations of the CPT and/or VD3. HPLC methods were developed and validated for quantitative determination of CPT and VD3 in the copolymer systems. To elucidate the synergistic effect of vitamin D3 on increasing the binding efficiency of CPT, different CPT to VD3 feed ratio were investigated. Results: The binding efficiency of VD3 in the presence of CPT. VD3 binding efficiency is not affected by the presence of the camptothecin, but is strongly affected by the nanospheres composition. Work was delayed by contract difficulties at Rutgers, but is now underway. SUBJECT TERMSNo subject terms were provided. I would like to bring to your attention that although this research project was awarded in August 2006 the actual award document was not issued until after April 2007. As a consequence, the actual research has begun just 3 month ago. We also applied for a one-year no-cost extension of funding that was accepted only on August 13, 2007. Thus, the below report is a summary of preliminary data obtained within 3.5 month of a research work. Introduction BackgroundSelf-assembling nanospheres offer a promising route to the delivery of pharmaceuticals that have poor bioavailability by improving the drugs' stability, circulation times in the body, and permeability through cell membranes, while reducing their toxicities.(1) Many drugs, including anti-tumor agents, anti-depressants and statins, are lipophilic and therefore require a solubilization process to enable their parenteral delivery. (2) Of the many alternative approac...

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Tethering poly(ethylene glycol)s to improve the surface biocompatibility of poly(acrylonitrile-co-maleic acid) asymmetric membranes

Cited by 81 publications

References 36 publications

A Microwell Cell Culture Platform for the Aggregation of Pancreatic β-Cells

A Microwell Cell Culture Platform for the Aggregation of Pancreatic β-Cells

Tethering methoxy polyethylene glycols to improve the antifouling property of PSF/PAA‐blended membranes

Nanospheric Chemotherapeutic and Chemoprotective Agents

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