Inclusion of organic components in culture medium to improve the in vitro propagation of Cattleya warscewiczii and Cattleya gaskelliana

Mercado, Seir Antonio Salazar; Jaimes, Yuri Manuelita Osorio

doi:10.1016/j.sajb.2022.05.002

Cited by 7 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…About doses of tetrazolium, the 1% dose presented the highest percentages of viability in all species, while the 0.2% dose presented the lowest percentages. This shows that the use of high concentrations of tetrazolium is preferable in the Capsicum genus as it occurs in other species (Mercado and Jaimes, 2022). Tetrazolium salts generates water-soluble sulfonated formazans, which are rapidly absorbed by cells and their concentration is directly proportional to the number of living cells in the seed embryo (Stockert et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Seed Viability, Germination, and Seedling Growth of Capsicum Cultivated Species

Corozo Quiñónez,

López-Cedeño,

Lascano-Borja

et al. 2023

Chil. j. agric. anim. sci.

View full text Add to dashboard Cite

Seed viability of domesticated and cultivated Capsicum species: Capsicum annuum L., Capsicum baccatum L., Capsicum chinense Jacq., Capsicum frutescens L., and Capsicum pubescens Ruiz and Pav. was tested by the tetrazolium method. The seeds were soaked in three tetrazolium salt solutions of 1, 0.5 and 0.2%, and temperature conditions of 30 - 35 C for 3 h. The percentage of seeds stained in relation to the total number of seeds was evaluated. To estimate germination, the seeds were subjected to chemical treatments with gibberellic acid (GA3) at doses of 0, 100, 200, 300, and 400 mg L-1 at room temperature for 24 h. The germination rate was estimated, while plant height was evaluated at 10, 20 and, 30 days after sowing (DAS). Capsicum seed viability fluctuated between 52.44% and 97.11%. The highest percentage of viability was obtained with the 1% dose of tetrazolium (81.63%), while the 0.2% dose resulted in the lowest average value (63.7%). In addition, C. annuum, C. baccatum and C. chinense showed high staining patterns, while most of the observed patterns in C. frutescens corresponded to non-viable seeds. After GA3 application, average seed germination was 100% in C. chinense; 75.6% in C. baccatum; 73.32% in C. annuum; and 61.5% in C. frutescens. However, the application of GA3 did not cause an additional effect on the germination of Capsicum seeds. The highest plant height at 30 DAS was observed in accessions of C. annuum (9.08 cm), while C. chinense presented the lowest value (4.49 cm).

show abstract

Section: Resultsmentioning

confidence: 99%

Seed Viability, Germination, and Seedling Growth of Capsicum Cultivated Species

Corozo Quiñónez,

López-Cedeño,

Lascano-Borja

et al. 2023

Chil. j. agric. anim. sci.

View full text Add to dashboard Cite

show abstract

“…In several studies sucrose solution (10 %) has had a positive effect on seed viability because sucrose is linked to the activation of metabolic processes related to dehydrogenase enzymes in cellular respiration, helping to maintain an osmotic balance in the seed, which prevents damage to the embryo (Mercado and Jaimes, 2022;Salazar and Botello, 2020;Salazar et al, 2019;Hosomi et al, 2017;Hosomi et al, 2012). Moreover, distilled water treatments resulted in low viability in the Castillo variety at several periods and tetrazolium concentrations.…”

Section: Pretreatment and Viability Of Seedsmentioning

confidence: 99%

Viability of seeds of two varieties of Coffea arabica L. using different pretreatments in the tetrazolium test

CONTRERAS

Rozo²,

Mercado³

2022

AAS

View full text Add to dashboard Cite

<p class="042abstractstekst">This research attempted to determine the efficacy of the tetrazolium test in the evaluation of the seed viability of two varieties of <em>Coffea arabica</em> L. (‘Castillo’ and ‘Cenicafé’). The fruits were obtained from crops located in the municipalities of Salazar de las Palmas and Arboledas (Norte de Santander - Colombia). The test was carried out with embryos manually extracted from the seeds using tweezers. Three pretreatments were established: distilled water, sodium hypochlorite (2.5 %), sucrose (10 %), and a control (no pretreatment). Embryos were placed in a cysteine solution (0.5 %) to prevent oxidation, then immersed in tetrazolium solutions with concentrations of 0.035 %, 0.075 %, and 0.1 % for a period of 6, 9, and 12 hours in darkness. The results of the viability test were validated with seed germination, using the wet paper towel method in darkness. The best viability percentages were found with the application of sodium hypochlorite (NaClO 2.5 %), with a high correlation with the germination percentage. The use of pretreatments improved the efficiency of the viability test and allowed the use of low concentrations of the reagent (0.035 %), giving the farmer a quick and less expensive alternative to determine germination capacity.</p>

show abstract

“…Organic additives contain carbohydrates, vitamins, and amino acids, which enhance the effectiveness of the propagation of orchid plants and decrease laboratory production costs (Moraes et al, 2020;Poniewozik, Szot, & Parzymies, 2021;Samiei et al, 2021). However, few studies have investigated the clonal micropropagation of C. gaskelliana (Mercado & Jaimes, 2022). Therefore, we developed an effective protocol for PLB proliferation, plantlet regeneration, and rooting of this species.…”

Section: Introductionmentioning

confidence: 99%

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

Hussien,

Molkanova,

Raeva-Bogoslovskaya

et al. 2024

Ciênc. agrotec.

View full text Add to dashboard Cite

In this study, a tissue culture method was developed for the propagation and conservation of Cattleya gaskelliana (N.E.Br.) B.S. Williams. Initially, protocorms with leaf primordium, obtained via asymbiotic seed germination, were used as explants. Half-strength Murashige and Skoog (MS) medium supplemented with 1.5 mg L−1 6-benzyl aminopurine (BA) and 2.0 mg L−1, indole butyric acid (IBA) was found to be optimal for the proliferation of protocorm-like bodies (PLB). Most explants (81%) proliferated, each producing 11.1 ± 1.87 units per explant. The optimum number of PLB differentiated to plantlets on a cultured medium with 0.5 mg L−1 Thidiazuron (TDZ) + 100 mL L−1 coconut water. Firmly rooted plantlets with vigorous growth were regenerated on ½ MS medium + 1.0 mg L−1 Indole- 3-acetic acid (IAA) and 50 g L−1 banana puree. Plants regenerated via in vitro processes were wrapped with sphagnum moss and acclimatized in a substrate containing equal proportions of bark, perlite, and peat with a 100% ex-vitro survival rate after acclimation for 70 days. The protocol developed in this study can be used to obtain several thousand plants within one year, and thus, is an effective method.

show abstract

Inclusion of organic components in culture medium to improve the in vitro propagation of Cattleya warscewiczii and Cattleya gaskelliana

Cited by 7 publications

References 50 publications

Seed Viability, Germination, and Seedling Growth of Capsicum Cultivated Species

Seed Viability, Germination, and Seedling Growth of Capsicum Cultivated Species

Viability of seeds of two varieties of Coffea arabica L. using different pretreatments in the tetrazolium test

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

Contact Info

Product

Resources

About