Incidence of Salmonella Enteritidis in chicken layer flocks in Turkey: Results by real-time polymerase chain reaction and International Organization for Standardization culture methods

Temelli, Seran; Kahya, Serpil; Eyigör, Ayşegül; Çarlı, K. Tayfun

doi:10.3382/ps.2010-00796

Cited by 13 publications

(16 citation statements)

References 30 publications

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“…Therefore, 41 samples positive by qPCR were not isolated by bacteriology. According to Temelli et al (2010), the marked presence of false negatives in conventional bacteriology is related to a large number of nonviable Salmonella in the samples or to a small amount of biological material. Other factors may be the overgrowth of lactose-fermenting bacteria, masking Salmonella spp.…”

Section: Resultsmentioning

confidence: 99%

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

Moraes

Duarte

Bastos

et al. 2016

Rev. Bras. Cienc. Avic.

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Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2%

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Section: Resultsmentioning

confidence: 99%

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

Moraes

Duarte

Bastos

et al. 2016

Rev. Bras. Cienc. Avic.

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“…Salmonella Enteritidis were not found in the analyzed samples, but Temelli et al (2010) have found Salmonella Enteritidis as the most isolated serovar 49/75 (65.3%) in samples of cloacal swabs. The absence of animal meal in feed composition during growing phase can justify absence of Salmonella sp.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens

et al. 2016

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This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases.

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Section: Introductionunclassified

“…Salmonella varlığının standart kültür metotları ile tespiti 5-11 gün sürmekte [2] bu nedenle etkenin özellikle yetiştirme dönemlerinde erken belirlenebilmesi ve gerekli önlemlerin alınabilmesi için daha kısa sürede sonuç veren hızlı ve ekonomik farklı deteksiyon sistemleri de kullanılmaktadır [3,4] . Kanatlı hayvanlara ait farklı örnek tiplerinde Salmonella aranması çalışmalarında, LightCycler SYBR Green I gerçek zamanlı polimeraz zincir reaksiyonu (LCSGIrPCR) sisteminin birçok kez güvenli olarak kullanıl-dığı bildirilmektedir [4][5][6] . Salmonella teşhisinde bir diğer gerçek zamanlı PCR sistemi olarak DNA'ya bağlanan boyalara alternatif bir sistem ile kullanıma hazır hedef-spesifik problu tabletleri içeren DuPont BAX Salmonella sisteminin (BAXrPCR) de çeşitli gıda, su, çevresel örnekler ve fekal örneklerde başarıyla kullanıldığı rapor edilmektedir [7][8][9][10][11][12] .…”

Section: Introductionunclassified

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Detection of Salmonella from Layer Flocks and Typing of the Isolates

Demirbilek¹,

Kesin²,

Temelli³

et al. 2014

Kafkas Univ Vet Fak Derg

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ÖzetÇalışmada, farklı yetiştirme dönemlerindeki yumurtacı tavuklarda Salmonella aranmasında farklı metotların değerlendirilmesi, elde edilen izolatların serotiplendirilmesi, ribotiplendirilmesi ve antibiyotiplendirilmesi ile Salmonella yaygınlığının belirlenmesi amaçlandı. Bu amaçla, 58 adet ticari yumurtacı tavuk kümesinden yetiştirmenin 15., 25. ve 40. haftalık dönemlerinde alınan toplam 174 adet drag svap örneği, Salmonella varlığı açısından standart kültür metodu olan ISO (ISO 6579:2002/Amd 1:2007 -ISO) ve 2 farklı gerçek zamanlı polimeraz zincir reaksiyonu (rPCR) sistemi (Light Cycler SYBR Green I rPCR -LCSGIrPCR ve DuPont BAX Q7 rPCR -BAXrPCR) kullanılarak analiz edildi. İncelenen 58 kümesin 17 adedi (%29.31), 174 adet drag svap örneğinin 15. ve 25. haftalık dönemlerde 6'şar (%3.44 ve %3.44), 40. haftada ise 10 adedi (%5.74) olmak üzere toplam 22'si (%12.62) her 3 metotla Salmonella yönünden pozitif olarak bulundu. Elde edilen 22 adet Salmonella izolatının 20'sinin (%90.90) Salmonella enterica subsp. enterica olup 9'unun Enteritidis (SE) (%40.9), 7'sinin Infantis (SI) (%31.8), 1'inin Hadar (%4.5), 1'inin Montevideo (%4.5), 1'inin Colombo (%4.5) ve 1'inin de Spartel (%4.5) serovarı olduğu, 2'sinin (%9.10) ise Salmonella enterica subsp. arizonae ile Salmonella enterica subsp. houtenae alttürlerine ait olduğu belirlendi. Ayrıca izolatların test edilen 24 antibiyotiğin 23'üne karşı direnç oluşturduğu, en yüksek direnç oranının Ampicillin (%100), Neomycin (%100), Penicillin G (%100) ve Erythromycin'e (%95.45) karşı olduğu saptandı. Çalışmanın sonucunda, yumurtacı tavuklarda Salmonella'nın hızlı deteksiyonunda LCSGIrPCR ve BAXrPCR sistemlerinin ISO metodunu destekleyici olarak kullanılabileceği, incelenen tüm yetiştirme dönemlerinde Salmonella enterica subsp. enterica varlığının yüksek olduğu belirlendi.Anahtar sözcükler : Salmonella, Yumurtacı tavuk, Drag svap, ISO, Real-time PCR, Serotiplendirme, Ribotiplendirme, Antibiyotiplendirme Detection of Salmonella from Layer Flocks and Typing of the Isolates AbstractThis study aimed to evaluate different detection methods in determining Salmonella prevalence in layers of various rearing periods, and to serotype, ribotype and antibiotype the isolates. For this, 174 drag swab samples taken from 58 commercial layer flocks of 15, 25, and 40 week age were analyzed for Salmonella by ISO standard culture method (ISO 6579:2002/Amd 1:2007 and by 2 real time polymerase chain reaction (rPCR) systems (Light Cycler SYBR Green I rPCR -LCSGIrPCR and DuPont BAX Q7 rPCR -BAXrPCR). Seventeen out of 58 (29.31%) flock samples, and 6 of the 15 th and 25 th weeks' (3.44% and 3.44%), and 10 (5.74%) of the 40 th week's drag swab samples were Salmonella positive by all 3 methods. Twenty out of 22 Salmonella isolates (90.90%) were Salmonella enterica subsp. enterica, including serovars of 9 Enteritidis (SE) (40.9%), 7 Infantis (SI) (31.8%), 1 Hadar (4.5%), 1 Montevideo (4.5%), 1 Colombo (4.5%), 1 Spartel (4.5%), and 2 subspecies (9.10%) as Salmonella enterica subsp. arizonae and Salmonell...

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Incidence of Salmonella Enteritidis in chicken layer flocks in Turkey: Results by real-time polymerase chain reaction and International Organization for Standardization culture methods

Cited by 13 publications

References 30 publications

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures

Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens

Detection of Salmonella from Layer Flocks and Typing of the Isolates

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