Incidence of leptospiral abortion in Brazilian dairy cattle

Langoni, Hélio; Souza, Luiz Carlos de; Silva, Aristeu Vieira da; Luvizotto, Maria Cecília Rui; Paes, Antônio Carlos; Lucheis, Simone Baldini

doi:10.1016/s0167-5877(99)00020-3

Cited by 40 publications

(33 citation statements)

References 5 publications

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“…por PCR em 3,6 % de amostras de 124 fetos analisados e provenientes de estados brasileiros. Já Langoni et al (1999) isolaram Leptospira sp. em 12,5 % dos 120 fetos analisados, em São Paulo.…”

Section: Discussionunclassified

Causas de aborto bovino diagnosticadas no Setor de Patologia Veterinária da UFRGS de 2003 a 2011

et al. 2013

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Descrevem-se as causas de aborto bovino diagnosticadas no Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul no período de janeiro de 2003 a dezembro de 2011. Um total de 490 fetos bovinos foi analisado neste período. Causas específicas de aborto foram encontradas em 46,7% dos casos. Infecções por protozoários, em especial Neospora caninum acometeram 33% dos casos (162/490). Bactérias com 6,3% (31/490), seguidas por fungos com 0,8% (4/490) dos casos, foram causas adicionais de abortos. Em dois fetos (0,4%), coinfecções por dois agentes foram identificadas. Causas não-infecciosas foram observadas em 3% dos abortos e Malformações congênitas em 2,6%.

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Section: Discussionunclassified

Causas de aborto bovino diagnosticadas no Setor de Patologia Veterinária da UFRGS de 2003 a 2011

et al. 2013

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“…This acute febrile disease results in hepatic and renal dysfunction and hemorrhagic disorders that can cause death in 5 to 10% of human cases (32,39) and fatality rates that are even higher in dogs. In livestock, Leptospira infection is associated with abortion, stillbirth, infertility, milk drop syndrome, and occasionally death (24,34,52).…”

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confidence: 99%

Protection againstLeptospira interrogansSensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1

Branger

Chatrenet

Gauvrit³

et al. 2005

Infect Immun

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The use of DNA constructs encoding leptospiral proteins is a promising new approach for vaccination against leptospirosis. In previous work we determined that immunization with hemolysis-associated protein 1 (Hap1) (LipL32) expressed by adenovirus induced significant protection against a virulent Leptospira challenge in gerbils. To avoid the use of the adenovirus vector, we checked for clinical protection against lethal challenge by DNA vaccination. A DNA vaccine expressing Hap1 was designed to enhance the direct gene transfer of this protein into gerbils. A challenge was performed 3 weeks after the last immunization with a virulent strain of serovar canicola. Our results show that the cross-protective effect with pathogenic strains of Leptospira, shared by Hap1, could be mediated by the DNA plasmid vector. This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.

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“…This acute febrile disease can progress to hepatic and renal dysfunction and hemorrhagic disorders that cause death in 5 to 10% of cases in humans (9,10,21) and are more lethal in dogs. In livestock, Leptospira infection is a frequent cause of abortion, stillbirth, infertility, milk drop syndrome, and (occasionally) death (4,13,23,25,31).…”

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Identification of the Hemolysis-Associated Protein 1 as a Cross-Protective Immunogen ofLeptospira interrogansby Adenovirus-Mediated Vaccination

et al. 2001

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New vaccine strategies are needed for the prevention of leptospirosis, a widespread human and animal disease caused by pathogenic leptospires. Our previous work determined that a protein leptospiral extract conferred cross-protection in a gerbil model of leptospirosis. The 31-to 34-kDa protein fraction of Leptospira interrogans serovar autumnalis was shown sufficient for this purpose. In the present study, N-terminal sequencing of a 32-kDa fraction and Southern blotting of genomic DNA with corresponding degenerated oligonucleotide probes identified two of its constituents: hemolysis-associated protein 1 (Hap1) and the outer membrane Leptospira protein 1 (OmpL1). Adenovirus-mediated Hap1 vaccination induces significant protection against a virulent heterologous Leptospira challenge in gerbils, whereas a similar OmpL1 construct failed to protect the animals. These data indicate that Hap1 could be a good candidate for developing a new generation of vaccines able to induce broad protection against leptospirosis disease.

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Incidence of leptospiral abortion in Brazilian dairy cattle

Cited by 40 publications

References 5 publications

Causas de aborto bovino diagnosticadas no Setor de Patologia Veterinária da UFRGS de 2003 a 2011

Causas de aborto bovino diagnosticadas no Setor de Patologia Veterinária da UFRGS de 2003 a 2011

Protection againstLeptospira interrogansSensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1

Identification of the Hemolysis-Associated Protein 1 as a Cross-Protective Immunogen ofLeptospira interrogansby Adenovirus-Mediated Vaccination

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