Inactivation of Viruses in Bubbling Processes Utilized for Personal Bioaerosol Monitoring

Agranovski, Igor E.; Safatov, A. S.; Borodulin, A.I.; Pyankov, Oleg V.; Petrishchenko, V. A.; Сергеев, А. Н.; Agafonov, A. P.; Ignatiev, G. M.; Agranovski, Victoria

doi:10.1128/aem.70.12.6963-6967.2004

Cited by 54 publications

(55 citation statements)

References 18 publications

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“…Of the 132 citations, 92 were excluded at the second relevance level after retrieving the articles, primarily due to a lack of environmental sampling or reporting only discovery and not persistence of the influenza virus. Other citations were excluded, as they reported virus stability in laboratory techniques (1,39,61,63), disinfection (15,23,54,62,86,103), persistence in eggs, meat, or carcasses (1,4,41,47,60,72), transmission rather than persistence (44,56,85), or only one sampling time (24,27,45,69,101).…”

Section: Resultsmentioning

confidence: 99%

Using the Systematic Review Methodology To Evaluate Factors That Influence the Persistence of Influenza Virus in Environmental Matrices

Irwin

Yoon

Wang

et al. 2011

Appl Environ Microbiol

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, i.e., air, water, soil, feces, and fomites. An electronic search of PubMed, CAB, AGRICOLA, Biosis, and Compendex was performed, and citation relevance was determined according to the aim of the review. Quality assessment of relevant studies was performed using criteria from experts in virology, disease ecology, and environmental science. A total of 9,760 abstracts were evaluated, and 40 appeared to report the persistence of influenza virus in environmental samples. Evaluation of full texts revealed that 19 of the 40 studies were suitable for review, as they described virus concentration measured at multiple sampling times, with viruses detectable at least twice. Seven studies reported persistence in air (six published before 1970), seven in water (five published after 1990), two in feces, and three on surfaces. All three fomite and five air studies addressed human influenza virus, and all water and feces studies pertained to avian influenza virus. Outcome measurements were transformed to half-lives, and resultant multivariate mixed linear regression models identified influenza virus surviving longer in water than in air. Temperature was a significant predictor of persistence over all matrices. Salinity and pH were significant predictors of persistence in water conditions. An assessment of the methodological quality review of the included studies revealed significant gaps in reporting critical aspects of study design.

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Section: Resultsmentioning

confidence: 99%

Using the Systematic Review Methodology To Evaluate Factors That Influence the Persistence of Influenza Virus in Environmental Matrices

Irwin

Yoon

Wang

et al. 2011

Appl Environ Microbiol

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“…The above-described collection fluid was chosen to maximize the viral recovery rate during the sampling procedure (Agranovski et al 2004a;Agranovski et al 2004b).…”

Section: Experimental Apparatus and Protocolmentioning

confidence: 99%

Long-Term Sampling of Viable Airborne Viruses

Agranovski

Safatov

Pyankov

et al. 2005

Aerosol Science and Technology

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A novel bioaerosol sampling technique, which utilizes the bubbling process in the collection fluid, has recently been developed and found feasible for a long-term personal sampling of airborne bacteria and fungal spores as it maintained high physical collection efficiency and high microbial recovery rate for robust and stresssensitive microorganisms. Further tests have shown that the new technique also has potential to collect viable airborne viruses, particularly when utilized for a short-term sampling of robust strains. As the short-term sampling has a limited application for assessing personal exposure in bioaerosol-contaminated environments, the present study was undertaken to investigate the feasibility of the "bubbler" for a long-term monitoring of viable airborne viruses. Liquid droplets containing Vaccinia virions (that simulate Variola, a causative agent of smallpox) were aerosolized with a Collison nebulizer into a 400-liter test chamber, from which the droplets were collected by three identical prototype personal samplers in the liquid medium during different time periods ranging from 1 to 6 hours. The viral content was measured in the collection fluid of the sampler and in the initial suspension of the nebulizer using the fluorescence-based method and by enumerating plaque-forming units per milliliter of the fluids. The relative recovery of viruses after the sampling act was determined. The results show that the "bubbling" technique has consistent collection efficiency over time and is capable of maintaining the viability of Vaccinia, for at least 6 hours, with a loss in recovery rate of about 10%. The data demonstrate a good potential of the new technique for measuring personal exposure to robust airborne viruses over a long period.

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“…Obviously, the sampling liquid from the personal bioaerosol sampler could be considered as a complex matrix. As an example of complex matrices, the optimally composed sampling liquid (Hank's solution supplemented with 2% FBS) for high survival of collected airborne microbes was described earlier by Agranovski et al (2004b). Besides, some mineral or biological particulate matter could be collected by the aerosol sampler and this resulting cocktail could also have an affect onto specific target detection by the SPR.…”

Section: Factors Influencing Bioaerosols Detection By Sprmentioning

confidence: 99%

Factors Influencing Rapid Detection of Bioaerosols by Surface Plasmon Resonance-Based Technique

Usachev

Usacheva

Agranovski

2014

Aerosol Science and Technology

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In bioaerosol monitoring applications, technologies allowing rapid and precise detection of airborne pathogens are highly demanded. One of such technologies, based on the immunoreaction-operating principle in nearly real-time mode without any specific labeling, is known as surface plasmon resonance (SPR). In previous studies, we have shown applicability of the SPR technology for rapid and selective detection of viral and bacterial aerosols where successful combination of the SPR machine with our earlier produced personal bioaerosol sampler opened new prospects in development of portable bioaerosol monitors. The current study is a logical continuation of our previous research dedicated to the technology development for rapid bioaerosol detection. Here, we focus on one of the main factors possibly influencing the SPRbased bioaerosol monitoring; the SPR performance on target bioaerosol detection was evaluated at conditions of substantial air contamination with different nontargeted microorganisms, commonly presented in the air. Besides, different compositions of sampling liquids were tested in regards to the SPR results interference. Our findings clearly verified high specificity of the technology even in cases of highly contaminated air environments with aerosols of biological and mineral origins. It was found that both nontargeted bioaerosols and nanosized aerosols of mineral background do not have significant influence on the specific SPR detection of targeted bioaerosols.

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Inactivation of Viruses in Bubbling Processes Utilized for Personal Bioaerosol Monitoring

Cited by 54 publications

References 18 publications

Using the Systematic Review Methodology To Evaluate Factors That Influence the Persistence of Influenza Virus in Environmental Matrices

Using the Systematic Review Methodology To Evaluate Factors That Influence the Persistence of Influenza Virus in Environmental Matrices

Long-Term Sampling of Viable Airborne Viruses

Factors Influencing Rapid Detection of Bioaerosols by Surface Plasmon Resonance-Based Technique

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