Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium in manure-amended soils studied in outdoor lysimeters

Nyberg, Karin; Vinnerås, Björn; Ottoson, Jakob; Aronsson, Pär; Albihn, Ann

doi:10.1016/j.apsoil.2010.10.004

Cited by 32 publications

(24 citation statements)

References 36 publications

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“…Minimal declines of low and high attO157 populations in DML-amended SIL soils occurred by 28 days, but declines of ca 2.4 and 2.5 log CFU/gdw in low and high populations, respectively, of attO157 in small containers occurred by 56 days. The different results seen in our study 1 and those seen in previously reported studies (12) for declines in E. coli O157:H7 populations in DML-amended soils may be due to the regular irrigation of soils in our small containers, which kept the soils moist and alleviated desiccation stress on attO157 populations. The results from study 1 showed statistical differences in the survival of E. coli in PLamended soils compared with unamended and DMLamended soils when conducted over 8 weeks.…”

Section: Discussioncontrasting

confidence: 98%

Survival and Persistence of Nonpathogenic Escherichia coli and Attenuated Escherichia coli O157:H7 in Soils Amended with Animal Manure in a Greenhouse Environment

Sharma

Millner

Hashem

et al. 2016

Journal of Food Protection

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Animal manure provides benefits to agriculture but may contain pathogens that contaminate ready-to-eat produce. U.S. Department of Agriculture National Organic Program standards include 90- or 120-day intervals between application of manure and harvest of crop to minimize risks of pathogen contamination of fresh produce. Data on factors affecting survival of Escherichia coli in soils under greenhouse conditions are needed. Three separate studies were conducted to evaluate survival of nonpathogenic E. coli (gEc) and attenuated E. coli O157:H7 (attO157) inoculated at either low (4 log CFU/ml) or high (6 log CFU/ml) populations over 56 days. Studies involved two pot sizes (small, 398 cm(3); large, 89 liters), three soil types (sandy loam, SL; clay loam, CL; silt loam, SIL), and four amendments (poultry litter, PL; dairy manure liquids, DML; horse manure, HM; unamended). Amendments were applied to the surface of the soil in either small or large containers. Study 1, conducted in regularly irrigated small containers, showed that populations of gEc and attO157 (2.84 to 2.88 log CFU/g) in PL-amended soils were significantly (P < 0.05) greater than those in DML-amended (0.29 to 0.32 log CFU/g [dry weight] [gdw]) or unamended (0.25 to 0.28 log CFU/gdw) soils; soil type did not affect E. coli survival. Results from study 2, in large pots with CL and SIL, showed that PL-amended soils supported significantly higher attO157 and gEc populations compared with HM-amended or unamended soils. Study 3 compared results from small and large containers that received high inoculum simultaneously. Overall, in both small and large containers, PLamended soils supported higher gEc and attO157 populations compared with HM-amended and unamended soils. Populations of attO157 were significantly greater in small containers (1.83 log CFU/gdw) than in large containers (0.65 log CFU/gdw) at week 8, perhaps because small containers received more regular irrigation than large pots. Regular irrigation of small pots may have affected E. coli persistence in manure-amended soils. Overall, PL-amended soils in both small and large containers supported E. coli survival at higher populations compared with DML-, HM-, or unamended soils.

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Section: Discussioncontrasting

confidence: 98%

Survival and Persistence of Nonpathogenic Escherichia coli and Attenuated Escherichia coli O157:H7 in Soils Amended with Animal Manure in a Greenhouse Environment

Sharma

Millner

Hashem

et al. 2016

Journal of Food Protection

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“…Most of studies describing the use of lime to inactivate pathogens showed promising results, but all of these studies used a pH above 11.0 (Bean et al 2007;Nyberg et al 2010). However, the present study showed that lower pH values can also be successfully used for the same propose.…”

Section: Pathogen Inactivation At Ph 100contrasting

confidence: 66%

Pathogen Inactivation and the Chemical Removal of Phosphorus from Swine Wastewater

Viancelli

Kunz

Fongaro

et al. 2015

Water Air Soil Pollut

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Inactivation of pathogens present in animal manure prior to land application has justified the use of advanced technologies. However, some alternatives are expensive or not effective due to the organic material and suspended solids present in the effluent (e.g., ozone, UV light). The use of hydrated lime (calcium hydroxide, Ca(OH) 2 ) is an attractive wastewater treatment option due to the ability of lime to kill pathogens and to extract phosphorus from manure at an alkaline pH. The present study aimed to evaluate the soluble phosphorus removal and pathogen inactivation (Escherichia coli, Salmonella enterica serovar typhymurium and Porcine circovirus type 2), in the liquid fraction and in the solid generated after Ca(OH) 2 addition in swine wastewater, exposed for 3 and 24 h at different pH conditions: 9.0, 9.5, and 10.0. The results showed the efficiency of pH elevation with Ca(OH) 2 in the removal of soluble P at pH 9.0 and the total inactivation of E. coli, Salmonella, and P. circovirus type 2 at pH 10.0. The liquid fraction (reuse water) could be safely used for cleaning the swine production facilities, and the solid fraction (precipitated P) could be used as a secondary product and fertilizer.

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“…The higher nutrient level due to the manure addition may increase the activity of the indigenous soil microbial community including bacterial predators (Garcia et al, 2010). In addition, Nyberg, Vinneras, Ottoson, Aronsson, and Albihn (2010) found that the survival (CFU) of S. Typhimurium was significantly influenced by the application of cattle, poultry or urine in a sandy soil. The fastest reduction of 1 log unit (T90) was found in urine-amended soil (2.0 days) whilst the same reduction took 8.7 days in cattle-manure amended soil, and 15.3 days in poultrymanure amended soil.…”

Section: Survival Of Salmonella In Soil According To Introduction Methodsmentioning

confidence: 96%

Soil survival of Salmonella and transfer to freshwater and fresh produce

Jacobsen

Bech

2012

Food Research International

134

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Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium in manure-amended soils studied in outdoor lysimeters

Cited by 32 publications

References 36 publications

Survival and Persistence of Nonpathogenic Escherichia coli and Attenuated Escherichia coli O157:H7 in Soils Amended with Animal Manure in a Greenhouse Environment

Survival and Persistence of Nonpathogenic Escherichia coli and Attenuated Escherichia coli O157:H7 in Soils Amended with Animal Manure in a Greenhouse Environment

Pathogen Inactivation and the Chemical Removal of Phosphorus from Swine Wastewater

Soil survival of Salmonella and transfer to freshwater and fresh produce

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