Inactivated rabies vaccine control and release:use of an ELISA method

Fournier-Caruana, Jacqueline; Poirier, Bertrand; Haond, Geneviève; Jallet, Corinne; Fuchs, Florence; Tordo, Noël; Perrin, Pierre

doi:10.1016/s1045-1056(02)00070-2

Cited by 36 publications

(14 citation statements)

References 12 publications

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“…NDVspecific and APV-specific IgA and IgG in the lachrymal fluid and tracheal washes were assayed using an indirect ELISA (Dhinakar Raj & Jones, 1996) except that the coating antigen used was either APV or NDV. Corrected optical density values were calculated by deducting the optical density values of non-antigen-coated wells from those of the test wells (Fournier-Caruana et al ., 2003).…”

Section: Methodsmentioning

confidence: 99%

Interaction between live avian pneumovirus and Newcastle disease virus vaccines in specific pathogen free chickens

Ganapathy

Cargill²,

Montiel³

et al. 2005

Avian Pathology

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One-day-old specific pathogen free White Leghorn chicks were vaccinated with live avian pneumovirus (APV) vaccine, live Newcastle disease virus (NDV) vaccine or both. At intervals up to 28 days after vaccination, distribution of the virus in the tissues was studied, together with humoral and mucosal antibody responses in lachrymal fluid and tracheal washes. APV vaccine was detected for almost twice as long in the dual vaccinates as in the single vaccinates. Higher numbers of isolations of ND virus vaccine were obtained from the dual rather than the single vaccinates at 7 days post-vaccination but the reverse occurred at 14 days. APV serum antibodies were significantly lower in the dual rather than the single vaccinates. However, there were similar levels of local APV-specific IgA in the lachrymal fluids of both single and dual APV vaccinates. NDV serum antibody titres in the dual vaccinates were significantly higher than in the singly NDVvaccinated chickens. It appears that simultaneous vaccination of chicks with live APV and NDV vaccines causes temporary suppression of APV vaccine proliferation and reduces humoral antibody responses to it, although the antibody response to NDV is enhanced.

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Section: Methodsmentioning

confidence: 99%

Interaction between live avian pneumovirus and Newcastle disease virus vaccines in specific pathogen free chickens

Ganapathy

Cargill²,

Montiel³

et al. 2005

Avian Pathology

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“…ELISA may be used for the determination of the attachment of neutralizing antibodies to viral epitopes after inactivation. This is used as quality control of the antigens of HIV [87,99], rabies virus [100,101] and PV vaccines [102]. However, for the previous methods, knowledge of the neutralizing epitopes is necessary.…”

Section: Expert Commentarymentioning

confidence: 99%

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

Delrue

Verzele

Madder

et al. 2012

Expert Review of Vaccines

156

150

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The aim of this review is to make researchers aware of the benefits of an efficient quality control system for prediction of a developed vaccine's efficacy. Two major goals should be addressed when inactivating a virus for vaccine purposes: first, the infectious virus should be inactivated completely in order to be safe, and second, the viral epitopes important for the induction of protective immunity should be conserved after inactivation in order to have an antigen of high quality. Therefore, some problems associated with the virus inactivation process, such as virus aggregate formation, protein crosslinking, protein denaturation and degradation should be addressed before testing an inactivated vaccine in vivo

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“…For HIV, the attachment of neutralizing antibodies to viral epitopes is determined after inactivation by an enzymelinked immuno sorbent assay (ELISA) [25,50]. ELISA as a tool for quality control of the antigen is also used for rabies virus [21,53] and poliovirus vaccines [43]. Quality control for PRRSV cannot be performed using the same methods as for HIV and influenza, because of the limited knowledge of PRRSV neutralizing epitopes.…”

Section: Introductionmentioning

confidence: 99%

Assessing the functionality of viral entry-associated domains of porcine reproductive and respiratory syndrome virus during inactivation procedures, a potential tool to optimize inactivated vaccines

2009

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-Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe economic losses in the pig industry worldwide. Currently, vaccines based on inactivated PRRSV provide limited protection of pigs against infection, most likely because viral epitopes associated with the induction of neutralizing antibodies are not or poorly conserved during inactivation. To analyze the effect of inactivation procedures on the interaction of PRRSV with receptors involved in virus entry, a new assay was set up in this study. Viral entry-associated domains are most likely important for the induction of neutralizing antibodies, since neutralizing antibodies block interaction of PRRSV with cellular receptors. To investigate the interaction of PRRSV with the cellular receptors upon different inactivation procedures, attachment to and internalization of inactivated PRRSV into macrophages were monitored. AT-2 could not inactivate PRRSV completely and is therefore not useful for vaccine development. PRRSV inactivated with ultraviolet light, binary ethyleneimine and gamma irradiation, which all mainly have an effect at the genomic level, showed no difference compared to control live virus at all levels of virus entry, whereas PRRSV treated with formaldehyde, glutaraldehyde and pH changes, which all have a modifying effect on proteins, was not able to internalize into macrophages anymore. These results suggest that inactivation with methods with a main effect on the viral genome preserve PRRSV entry-associated domains and are useful for future development of an effective inactivated vaccine against PRRSV. Although PRRSV incubation at 37°C can completely inactivate PRRSV with preservation of entry-associated domains, this method is not recommended for vaccine development, since the mechanism is yet unknown.PRRSV / inactivation / vaccine / entry-associated domain / sialoadhesin

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Inactivated rabies vaccine control and release:use of an ELISA method

Cited by 36 publications

References 12 publications

Interaction between live avian pneumovirus and Newcastle disease virus vaccines in specific pathogen free chickens

Interaction between live avian pneumovirus and Newcastle disease virus vaccines in specific pathogen free chickens

Inactivated virus vaccines from chemistry to prophylaxis: merits, risks and challenges

Assessing the functionality of viral entry-associated domains of porcine reproductive and respiratory syndrome virus during inactivation procedures, a potential tool to optimize inactivated vaccines

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