In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

Cunha, Marcelo Rodrigues da; Alves, Marianna Carla; Calegari, Amanda Regina Alves; Iatecola, Amilton; Galdeano, Ewerton Alexandre; Galdeano, Talita Lopes; Munhoz, Marcelo de Azevedo e Souza; Plepis, Ana Maria de Guzzi; Martins, Virgínia da Conceição Amaro; Horn, Marilia M.

doi:10.1590/1980-5373-mr-2016-1112

Cited by 17 publications

(16 citation statements)

References 33 publications

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“…When stained with XP and TB, porous samples were comparable to the dense materials, suggesting the use of these samples for tissue engineering. Porous samples are best suited for applications in tissue engineering 28 because of their larger surface area, providing ample space for cell and vascular invasion 14,24 . In the porous samples, the cells spread across the surface and into the pores.…”

Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

et al. 2019

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The objective of this study was to evaluate Vero cells in in vitro biocompatibility tests with pure PHBV and PCL and 75/25 and 50/50 blends developed for bone tissue bioengineering in dense or porous forms. The biomaterials were characterized morphologically by scanning electron microscopy, (SEM) stereoscopic imaging (SI), and micrometer measurement. Cellular assay was made by MTT, morphological and cytochemistry analysis. The dense and porous scaffolds presented irregular surfaces. SEM allowed to observe details of the irregular surface throughout the dense samples and confirmed the absence of pores. It was found interconnected pores on porous samples. The porous samples were thicker than the dense samples. None of the polymers was considered toxic. Lower activity measured by MTT was observed for the 50/50 blends, suggesting a slower cell adhesion pattern. The cells were able to spread almost throughout the surface of the polymers, even into the pores. The dense and porous 50/50 samples were so brittle and difficult handling. Except for the 50/50 blends, the polymers were generally good substrates for the cells, were non-toxic, and exhibited the recommended morphological features. In addition, these biomaterials did not inhibit specific biological responses identified at the cytochemical level.

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Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

et al. 2019

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“…Modifications in aECM composed of collagen/elastin have been described as an approach to control the in vivo degradation of these matrices, providing better integration into the recipient tissue. These alterations include the chemical modification of collagen that results in positively or negatively charged matrices [ 23 , 28 ]. In the present study, macroscopic inspection of the wound area in the groups implanted with the matrices showed fragmentation and absorption of part of the biomaterial, especially of the anionic matrices.…”

Section: Discussionmentioning

confidence: 99%

“…The animals were anesthetized by gluteal intramuscular injection of an anesthetic solution (1:1) of Coopazine (xylazine) and Dopalen (ketamine) at a dose of 0.01 mg/10 g body weight [ 27 ]. After skin incision in the skull cap exposing the parietal bone, a 1-mm thick defect measuring 5 mm in diameter was created with a surgical drill coupled to a hand-held motor [ 28 ]. After surgery, the periosteum and skin were repositioned and sutured with 5.0 silk suture.…”

Section: Methodsmentioning

confidence: 99%

Use of an anionic collagen matrix made from bovine intestinal serosa for in vivo repair of cranial defects

et al. 2018

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Polymeric biomaterials composed of extracellular matrix components possess osteoconductive capacity that is essential for bone healing. The presence of collagen and the ability to undergo physicochemical modifications render these materials a suitable alternative in bone regenerative therapies. The objective of this study was to evaluate the osteogenic capacity of collagen-based matrices (native and anionic after alkaline hydrolysis) made from bovine intestinal serosa (MBIS). Twenty-five animals underwent surgery to create a cranial defect to be filled with native and anionic collagen matrixes, mmineralized and non mineralized. The animals were killed painlessly 6 weeks after surgery and samples of the wound area were submitted to routine histology and morphometric analysis. In the surgical area there was new bone formation projecting from the margins to the center of the defect. More marked bone neoformation occurred in the anionic matrices groups in such a way that permitted union of the opposite margins of the bone defect. The newly formed bone matrix exhibited good optical density of type I collagen fibers. Immunoexpression of osteocalcin by osteocytes was observed in the newly formed bone. Morphometric analysis showed a greater bone volume in the groups receiving the anionic matrices compared to the native membranes. Mineralization of the biomaterial did not increase its osteoregenerative capacity. In conclusion, the anionic matrix exhibits osteoregenerative capacity and is suitable for bone reconstruction therapies.

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“…Chitosan is a natural polymer derived from the polysaccharide chitin. Using chitosan alone as a scaffold in bone defects did not yield successful results in animal studies 101 . However, more promising results were obtained after combination with osteoinductive factors like BMP‐2 102 .…”

Section: Classificationmentioning

confidence: 99%

A novel classification of bone graft materials

2022

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Over the past few decades, the field of biomaterials concerning bone tissue engineering has gained a significant amount of interest. This has led to new biomaterials to be used as bone substitute materials. Despite the rapid increase in the types and forms of bone substitutes, a comprehensive classification encompassing all types of bone graft materials that have so far been developed and evaluated is lacking. Therefore, this review aims to integrate and bring together the published data on bone substitutes within the last 5 years and produce a novel classification that would provide bone material researchers with a better understanding of what materials have so far been used and evaluated and the areas, where research is lacking and deserves more attention. The literature available in all major databases was obtained and filtered using an elimination criterion to extract all the articles related to studies that tested bone substitute materials in nonclinical and clinical trials over the last 5 years. The review article would provide bone material researchers with an insight into the materials that have been evaluated for bone tissue engineering applications and identify future perspectives for bone graft material research.

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In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

Cited by 17 publications

References 33 publications

In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

Use of an anionic collagen matrix made from bovine intestinal serosa for in vivo repair of cranial defects

A novel classification of bone graft materials

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