In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

Baptista-Perianes, Amália; Malmonge, Sônia Maria; Simbara, Márcia Mayumi Omi; Santos, Arnaldo Rodrigues

doi:10.1590/1980-5373-mr-2019-0338

Cited by 11 publications

(8 citation statements)

References 19 publications

(23 reference statements)

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“…More recently, extensive cytochemical evaluation has been conducted with fibroblastic cells grown in PHBV/PCL blends. The results presented herein are consistent with those previously described [30].…”

Section: Discussionsupporting

confidence: 93%

Osteogenic differentiation of rat bone mesenchymal stem cells cultured on poly (hydroxybutyrate-co-hydroxyvalerate), poly (ε-caprolactone) scaffolds

Rodrigues

Batista

Malmonge

et al. 2021

J Mater Sci: Mater Med

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Bioresorbable biomaterials can fill bone defects and act as temporary scaffold to recruit MSCs to stimulate their differentiation. Among the different bioresorbable polymers studied, this work focuses on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL). Were prepared blends of PHBV and PCL to obtain PHBV based biomaterials with good tenacity, important for bone tissue repair, associated with biocompatible properties of PCL. This study assesses the viability of Vero cells on scaffolds of PHBV, PCL, and their blends and the osteogenic differentiation of mesenchymal stem cells (MSCs). Materials were characterized in surface morphology, DSC and Impact Strength (IS). Vero cells and MSCs were assessed by MTT assay, cytochemical and SEM analysis. MSC osteogenic differentiation was evaluated through alizarin red staining and ALP activity. We found some roughness onto surface materials. DSC showed that the blends presented two distinct melting peaks, characteristic of immiscible blends. IS test confirmed that PHBV-PCL blends is an alternative for increase the tenacity of PHBV. MTT assay showed cells with high metabolic activities on extract toxicity test, but with low activity in the direct contact test. SEM analysis showed spreading cells with irregular and flattened morphology on different substrates. Cytochemical study revealed that MSCs maintained their morphology, although in smaller number for MSCs. The development of nodules of mineralized organic matrix in MSC cultures was identified by alizarin red staining and osteogenic differentiation was confirmed by the quantification of ALP activity. Thus, our scaffolds did not interfere on viability of Vero cells or the osteogenic differentiation of MSCs.

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Section: Discussionsupporting

confidence: 93%

Osteogenic differentiation of rat bone mesenchymal stem cells cultured on poly (hydroxybutyrate-co-hydroxyvalerate), poly (ε-caprolactone) scaffolds

Rodrigues

Batista

Malmonge

et al. 2021

J Mater Sci: Mater Med

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“…16 In the direct contact test, the morphology of Vero cells observed under the control conditions is compatible with previously published data. 49,50 The cells growing around the hydrogels also resembled those of the controls. The cells were found to grow around the CHI drops in the form of monolayers and, eventually, multiple layers.…”

Section: Discussionmentioning

confidence: 78%

Development of a chitosan and hyaluronic acid hydrogel with potential for bioprinting utilization: A preliminary study

2021

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Bioprinting is a technique that has been applied in the areas of tissue engineering and regenerative medicine (TERM). Natural polymer-based hydrogels are known for their favorable biocompatible properties, as well as attractive biomaterials for cell encapsulation. These hydrogels provide an aqueous three-dimensional environment with biologically relevant chemical and physical signals, mimicking the natural environment of the extracellular matrix (ECM). Chitosan (CHI) and hyaluronic acid (HA) have been widely researched for biomedical applications. Bioinks are “ink” formulations, usually hydrogels, that allow the printing of living cells. This work proposes the development of a low cost and simple chitosan CHI-AH hydrogel with potential to become a bioink. At physiological temperature, the biomaterials form a hydrogel. The material developed was characterized by the analysis of morphology, cytotoxicity, and cell viability. FTIR showed the characteristic vibrational bands of chitosan and HA. No difference in swelling was observed between the different formulations studied, although SEM showed architectural differences between the hydrogels obtained. Extract cytotoxicity testing showed that the hydrogel is not cytotoxic. The direct toxicity test also revealed the absence of toxicity, but the cells had difficulty migrating into the gel, probably because of its density. These data were confirmed by SEM. Further testing are ongoing to better understand the gel’s characteristics to improve the limitations found so far.

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“…PHBV is more fragile and has lower rate of hydrolytic degradation due to its higher degree of crystallinity. The blends of PCL and PHBV could provide the compromise properties that suitable for use as scaffolds [9,18].…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Kosorn

Wutticharoenmongkol

2021

International Journal of Polymer Science

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The scaffolds of poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blends were fabricated from fused deposition modeling. From indirect cytotoxicity testing based on mouse fibroblasts, all scaffolds with various blend ratios were nontoxic to cells. The surface-treated scaffold with a blend ratio of 25/75 PCL/PHBV exhibited the highest proliferation of porcine chondrocytes and total glycosaminoglycans (GAGs) after 21 days of culture. The scaffolds with a blend ratio of 25/75 with local pores (LP) were prepared from FDM along with a salt leaching technique using NaCl as porogens. The effect of NaOH in surface treatment on the biological property of scaffolds was investigated. The scaffolds with LP and with 1 M NaOH surface treatment exhibited the highest proliferation of cells and total GAGs after 28 days of culture. The degradation behaviors of the scaffolds were studied. The nonsurface treated, surface treated without LP, and surface treated with LP scaffolds were degraded in phosphate buffer (pH 7.4) for 30 days at 37°C and 50°C for nonenzymatic condition and at 37°C for enzymatic condition. The surface treated with LP scaffold showed the highest amount of weight loss, followed by the surface treated without LP, and the nonsurface-treated scaffolds without LP, respectively. The results from Fourier-transform infrared spectroscopy indicated degradation of PCL and PHBV through hydrolysis of the ester functional group. The compressive strengths of all scaffolds were sufficiently high. The results suggested that the scaffolds with the existence of LP and with surface treatment showed the highest potential for use as cartilage scaffolds.

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In vitro Evaluation of PHBV/PCL Blends for Bone Tissue Engineering

Cited by 11 publications

References 19 publications

Osteogenic differentiation of rat bone mesenchymal stem cells cultured on poly (hydroxybutyrate-co-hydroxyvalerate), poly (ε-caprolactone) scaffolds

Osteogenic differentiation of rat bone mesenchymal stem cells cultured on poly (hydroxybutyrate-co-hydroxyvalerate), poly (ε-caprolactone) scaffolds

Development of a chitosan and hyaluronic acid hydrogel with potential for bioprinting utilization: A preliminary study

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

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