In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance—A model study in chickens

Marosevic, Durdica; Cervinkova, D.; Vlkova, H.; Vídeňská, Petra; Babák, Vladimír; Jaglič, Z.

doi:10.1016/j.vetmic.2013.12.017

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…As carbapenems are not licensed for use in livestock, it is assumed that the occurrence of carbapenemase-producing bacteria is triggered by coselective pressure, since plasmids carrying bla NDM-1 , like the plasmid chosen for use in this study, commonly harbor multiple but variable resistance determinants ( 16 , 22 ). Still, current research shows that the spread of certain plasmid-mediated resistance genes in broiler chickens is also possible without antibiotic selective pressure ( 20 , 23 ). Therefore, for understanding the mechanisms contributing to the spread of carbapenem resistance or carbapenem-nonsusceptible isolates in vivo , the objective of our animal trial was to explore the broad-host-range capacity and stability of a conjugative bla NDM-1 -carrying plasmid, IncA/C 2 plasmid pRH-1238, hosted by an S .…”

Section: Discussionmentioning

confidence: 99%

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In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Hadžiabdić

Fischer

Malorny

et al. 2018

Antimicrob Agents Chemother

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The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals pose an important safety concern for public health. With our in vivo broiler chicken infection study, we investigated the transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella enterica subsp. enterica serovar Corvallis without inducing antibiotic selection pressure. We evaluated the dependency of the time point of inoculation on donor (S. Corvallis [12-SA01738]) and plasmid-free Salmonella recipient [d-tartrate-fermenting (d-Ta+) S. Paratyphi B (13-SA01617), referred to here as S. Paratyphi B (d-Ta+)] excretion by quantifying their excretion dynamics. Using plasmid profiling by S1 nuclease-restricted pulsed-field gel electrophoresis, we gained insight into the variability of the native plasmid content among S. Corvallis reisolates as well as plasmid acquisition in S. Paratyphi B (d-Ta+) and the enterobacterial gut microflora. Whole-genome sequencing enabled us to gain an in-depth insight into the microevolution of plasmid pRH-1238 in S. Corvallis and enterobacterial recipient isolates. Our study revealed that the fecal excretion of avian native carbapenemase-producing S. Corvallis is significantly higher than that of S. Paratyphi (d-Ta+) and is not hampered by S. Paratyphi (d-Ta+). Acquisition of pRH-1238 in other Enterobacteriaceae and several events of plasmid pRH-1238 transfer to different Escherichia coli sequence types and Klebsiella pneumoniae demonstrated an interspecies broad host range. Regardless of the microevolutionary structural deletions in pRH-1238, the single carbapenem resistance marker blaNDM-1 was maintained on pRH-1238 throughout the trial. Furthermore, we showed the importance of the gut E. coli population as a vector of pRH-1238. In a potential scenario of the introduction of NDM-1-producing S. Corvallis into a broiler flock, the pRH-1238 plasmid could persist and spread to a broad host range even in the absence of antibiotic pressure.

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Section: Discussionmentioning

confidence: 99%

“…Commercial poultry production is a continuously evolving livestock branch, characterized by fast turnovers and production pressure, which, combined with poor management, could lead to the misusage of antimicrobials ( 20 ). This might also favor commercial broiler production acting as a niche for selection of multidrug-resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Hadžiabdić

Fischer

Malorny

et al. 2018

Antimicrob Agents Chemother

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“…in the presence of relatively high numbers of potential recipients and in the absence of competitive microflora. A recent in vivo study performed on commercial chickens has shown that the spread of a plasmidcarried erm(B) gene occurs not only within the faecal microbiota under both specific (tylosin and lincomycin) and non-specific (chlortetracycline) antibiotic pressure, but also in the absence of any antimicrobial pressure [63]. Moreover, all isolates of enterococci and streptococci that acquired the erm(B) gene were also resistant to tetracycline, which indicates that tetracycline may co-select for MLS B resistance.…”

Section: Transmission Of Tetracycline and Mls Resistancementioning

confidence: 99%

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Marosevic¹,

Kaevska²,

Jaglič³

2017

Ann Agric Environ Med.

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Marosevic D, Kaevska M, Jaglic Z. Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage -a review. Ann Agric Environ Med. 2017; 24(2): 338-344. https://doi.org/10.26444/aaem/74718 AbstractAn excessive use of antimicrobial agents poses a risk for the selection of resistant bacteria. Of particular interest are antibiotics that have large consumption rates in both veterinary and human medicine, such as the tetracyclines and macrolidelincosamide-streptogramin (MLS) group of antibiotics. A high load of these agents increases the risk of transmission of resistant bacteria and/or resistance determinants to humans, leading to a subsequent therapeutic failure. An increasing incidence of bacteria resistant to both tetracyclines and MLS antibiotics has been recently observed. This review summarizes the current knowledge on different tetracycline and MLS resistance genes that can be linked together on transposable elements.

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“…Esto hace que sea importante considerar que se pueden seleccionar resistencias a antibióticos para los que no se ha aplicado presión selectiva mediante la co-selección de genes de resistencia. De hecho, Marosevic et al (2014) observaron una expansión de determinantes genéticos para la resistencia a MLS tanto en grupos de pollos tratados con antibióticos pertenecientes a este grupo (lo que conformarían una presión selectiva) como con antibióticos de diferente clase.…”

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Estudio de la transmisión de resistencias a antibióticos mediante métodos moleculares en el sector avícola y su implicación para la salud pública

Penadés¹

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Me gustaría empezar dando las gracias a mis directoras, las doctoras Ana Jiménez y María Antonia Ferrús, tanto por haberme aceptado para la realizar los estudios bajo su dirección como por su ayuda y el tiempo que han dedicado en todas las etapas de la tesis. Aparte de mis directoras, esta tesis ha sido posible gracias al trabajo de la doctora Eva Doménech, que fue con quien se empezó el estudio preliminar que ha llevado a esta tesis y que ha sido de gran ayuda para la interpretación de parte de los resultados y las publicaciones derivadas. Me gustaría también agradecer el trabajo del personal de laboratorio, Nancy, Ana y demás miembros del departamento, gracias a quienes he podido disponer de todo el material necesario para los ensayos y que me han ayudado con cualquier problema que haya podido surgir. Agradezco también la ayuda de mis amigos y compañeros de laboratorio, Aya y Miguel, con quienes los días han sido más entretenidos y siempre han estado dispuestos a ayudar en cualquier cosa. Por último, agradezco y dedico la tesis a mis padres, quienes me han apoyado durante esta larga etapa y que sin ellos nada hubiera sido posible. Els resultats de la present tesi mostren la presència de bacteris resistents als antibiòtics tant en gallines com en productes avícoles (ous en aquest cas) i que, en el cas d'ous, aquestes poden ser més diverses en ous ecològics que en ous de producció convencional. També s'ha vist com el tractament amb un sol antibiòtic afavoreix la selecció de resistències a antibiòtics de diferents famílies i s'ha demostrat també l'elevada presencia resistències a antibiòtics en E. coli aïllats de gallines d'1 dia de vida, incloent resistències a cefalosporines. Per últim, també s'ha comprovat com els gens de les famílies SHV i TEM, així com els qnrB i qnrS s'observen amb freqüència en E. coli no-susceptibles a cefalosporines o ciprofloxací, a més de l'elevada freqüència dels gens ermB, tetK, tetL i tetM en aïllats d'enterococs procedents de gallines.

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In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance—A model study in chickens

Cited by 6 publications

References 30 publications

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Estudio de la transmisión de resistencias a antibióticos mediante métodos moleculares en el sector avícola y su implicación para la salud pública

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In vivo spread of macrolide-lincosamide-streptogramin B (MLSB) resistance—A model study in chickens

Cited by 6 publications

References 30 publications

In Vivo Transfer and Microevolution of Avian Native IncA/C 2 bla NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

In Vivo Transfer and Microevolution of Avian Native IncA/C 2 bla NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Estudio de la transmisión de resistencias a antibióticos mediante métodos moleculares en el sector avícola y su implicación para la salud pública

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In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study